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Diagnostic accuracy of “sweeping” method compared to conventional sampling in rapid urease test for Helicobacter pylori detection in atrophic mucosa

Although the rapid urease test (RUT) is a simple method for detecting Helicobacter pylori (H. pylori) infection, it requires sufficient biopsy samples and its sensitivity varies depending on the site and condition of H. pylori infection. We compared the diagnostic performance of a “sweeping method”...

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Autores principales: Noh, Choong-Kyun, Lee, Gil Ho, Park, Jin Woong, Roh, Jin, Han, Jae Ho, Lee, Eunyoung, Park, Bumhee, Lim, Sun Gyo, Shin, Sung Jae, Cheong, Jae Youn, Kim, Jin Hong, Lee, Kee Myung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595103/
https://www.ncbi.nlm.nih.gov/pubmed/33116260
http://dx.doi.org/10.1038/s41598-020-75528-1
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author Noh, Choong-Kyun
Lee, Gil Ho
Park, Jin Woong
Roh, Jin
Han, Jae Ho
Lee, Eunyoung
Park, Bumhee
Lim, Sun Gyo
Shin, Sung Jae
Cheong, Jae Youn
Kim, Jin Hong
Lee, Kee Myung
author_facet Noh, Choong-Kyun
Lee, Gil Ho
Park, Jin Woong
Roh, Jin
Han, Jae Ho
Lee, Eunyoung
Park, Bumhee
Lim, Sun Gyo
Shin, Sung Jae
Cheong, Jae Youn
Kim, Jin Hong
Lee, Kee Myung
author_sort Noh, Choong-Kyun
collection PubMed
description Although the rapid urease test (RUT) is a simple method for detecting Helicobacter pylori (H. pylori) infection, it requires sufficient biopsy samples and its sensitivity varies depending on the site and condition of H. pylori infection. We compared the diagnostic performance of a “sweeping method” for H. pylori detection with the conventional biopsy sampling method in atrophic gastric conditions which can reduce RUT accuracy. This prospective study included 279 patients who underwent upper endoscopy to determine the presence of H. pylori infection. Gastric mucosa of both the antrum and the corpus were swabbed, and we named this method the “sweeping method”. Biopsy sampling for the conventional method, histologic evaluation, and polymerase chain reaction were performed at the same time. The sensitivity, specificity, and accuracy of the sweeping method were 0.941, 0.826, and 0.903, respectively, compared to 0.685, 0.859, and 0.742, respectively, for the conventional biopsy method. The area under the receiver operating curve for the sweeping method was 0.884 versus 0.772 for the conventional method (P < 0.001). The sweeping method had a faster detection time than the conventional method. Compared to conventional biopsy sampling, the sweeping method with the RUT provided higher sensitivity and accuracy for the detection of H. pylori, with a faster detection time.
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spelling pubmed-75951032020-10-29 Diagnostic accuracy of “sweeping” method compared to conventional sampling in rapid urease test for Helicobacter pylori detection in atrophic mucosa Noh, Choong-Kyun Lee, Gil Ho Park, Jin Woong Roh, Jin Han, Jae Ho Lee, Eunyoung Park, Bumhee Lim, Sun Gyo Shin, Sung Jae Cheong, Jae Youn Kim, Jin Hong Lee, Kee Myung Sci Rep Article Although the rapid urease test (RUT) is a simple method for detecting Helicobacter pylori (H. pylori) infection, it requires sufficient biopsy samples and its sensitivity varies depending on the site and condition of H. pylori infection. We compared the diagnostic performance of a “sweeping method” for H. pylori detection with the conventional biopsy sampling method in atrophic gastric conditions which can reduce RUT accuracy. This prospective study included 279 patients who underwent upper endoscopy to determine the presence of H. pylori infection. Gastric mucosa of both the antrum and the corpus were swabbed, and we named this method the “sweeping method”. Biopsy sampling for the conventional method, histologic evaluation, and polymerase chain reaction were performed at the same time. The sensitivity, specificity, and accuracy of the sweeping method were 0.941, 0.826, and 0.903, respectively, compared to 0.685, 0.859, and 0.742, respectively, for the conventional biopsy method. The area under the receiver operating curve for the sweeping method was 0.884 versus 0.772 for the conventional method (P < 0.001). The sweeping method had a faster detection time than the conventional method. Compared to conventional biopsy sampling, the sweeping method with the RUT provided higher sensitivity and accuracy for the detection of H. pylori, with a faster detection time. Nature Publishing Group UK 2020-10-28 /pmc/articles/PMC7595103/ /pubmed/33116260 http://dx.doi.org/10.1038/s41598-020-75528-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Noh, Choong-Kyun
Lee, Gil Ho
Park, Jin Woong
Roh, Jin
Han, Jae Ho
Lee, Eunyoung
Park, Bumhee
Lim, Sun Gyo
Shin, Sung Jae
Cheong, Jae Youn
Kim, Jin Hong
Lee, Kee Myung
Diagnostic accuracy of “sweeping” method compared to conventional sampling in rapid urease test for Helicobacter pylori detection in atrophic mucosa
title Diagnostic accuracy of “sweeping” method compared to conventional sampling in rapid urease test for Helicobacter pylori detection in atrophic mucosa
title_full Diagnostic accuracy of “sweeping” method compared to conventional sampling in rapid urease test for Helicobacter pylori detection in atrophic mucosa
title_fullStr Diagnostic accuracy of “sweeping” method compared to conventional sampling in rapid urease test for Helicobacter pylori detection in atrophic mucosa
title_full_unstemmed Diagnostic accuracy of “sweeping” method compared to conventional sampling in rapid urease test for Helicobacter pylori detection in atrophic mucosa
title_short Diagnostic accuracy of “sweeping” method compared to conventional sampling in rapid urease test for Helicobacter pylori detection in atrophic mucosa
title_sort diagnostic accuracy of “sweeping” method compared to conventional sampling in rapid urease test for helicobacter pylori detection in atrophic mucosa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595103/
https://www.ncbi.nlm.nih.gov/pubmed/33116260
http://dx.doi.org/10.1038/s41598-020-75528-1
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