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Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates

Cell development and behavior are driven by internal genetic programming, but the external microenvironment is increasingly recognized as a significant factor in cell differentiation, migration, and in the case of cancer, metastatic progression. Yet it remains unclear how the microenvironment influe...

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Autores principales: Chou, Szu-Yuan, Lin, Chang-You, Cassino, Theresa, Wan, Li, LeDuc, Philip R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595178/
https://www.ncbi.nlm.nih.gov/pubmed/33116169
http://dx.doi.org/10.1038/s41598-020-74575-y
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author Chou, Szu-Yuan
Lin, Chang-You
Cassino, Theresa
Wan, Li
LeDuc, Philip R.
author_facet Chou, Szu-Yuan
Lin, Chang-You
Cassino, Theresa
Wan, Li
LeDuc, Philip R.
author_sort Chou, Szu-Yuan
collection PubMed
description Cell development and behavior are driven by internal genetic programming, but the external microenvironment is increasingly recognized as a significant factor in cell differentiation, migration, and in the case of cancer, metastatic progression. Yet it remains unclear how the microenvironment influences cell processes, especially when examining cell motility. One factor that affects cell motility is cell mechanics, which is known to be related to substrate stiffness. Examining how cells interact with each other in response to mechanically differential substrates would allow an increased understanding of their coordinated cell motility. In order to probe the effect of substrate stiffness on tumor related cells in greater detail, we created hard–soft–hard (HSH) polydimethylsiloxane (PDMS) substrates with alternating regions of different stiffness (200 and 800 kPa). We then cultured WI-38 fibroblasts and A549 epithelial cells to probe their motile response to the substrates. We found that when the 2 cell types were exposed simultaneously to the same substrate, fibroblasts moved at an increased speed over epithelial cells. Furthermore, the HSH substrate allowed us to physically guide and separate the different cell types based on their relative motile speed. We believe that this method and results will be important in a diversity of areas including mechanical microenvironment, cell motility, and cancer biology.
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spelling pubmed-75951782020-10-29 Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates Chou, Szu-Yuan Lin, Chang-You Cassino, Theresa Wan, Li LeDuc, Philip R. Sci Rep Article Cell development and behavior are driven by internal genetic programming, but the external microenvironment is increasingly recognized as a significant factor in cell differentiation, migration, and in the case of cancer, metastatic progression. Yet it remains unclear how the microenvironment influences cell processes, especially when examining cell motility. One factor that affects cell motility is cell mechanics, which is known to be related to substrate stiffness. Examining how cells interact with each other in response to mechanically differential substrates would allow an increased understanding of their coordinated cell motility. In order to probe the effect of substrate stiffness on tumor related cells in greater detail, we created hard–soft–hard (HSH) polydimethylsiloxane (PDMS) substrates with alternating regions of different stiffness (200 and 800 kPa). We then cultured WI-38 fibroblasts and A549 epithelial cells to probe their motile response to the substrates. We found that when the 2 cell types were exposed simultaneously to the same substrate, fibroblasts moved at an increased speed over epithelial cells. Furthermore, the HSH substrate allowed us to physically guide and separate the different cell types based on their relative motile speed. We believe that this method and results will be important in a diversity of areas including mechanical microenvironment, cell motility, and cancer biology. Nature Publishing Group UK 2020-10-28 /pmc/articles/PMC7595178/ /pubmed/33116169 http://dx.doi.org/10.1038/s41598-020-74575-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chou, Szu-Yuan
Lin, Chang-You
Cassino, Theresa
Wan, Li
LeDuc, Philip R.
Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates
title Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates
title_full Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates
title_fullStr Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates
title_full_unstemmed Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates
title_short Probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates
title_sort probing coordinated co-culture cancer related motility through differential micro-compartmentalized elastic substrates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595178/
https://www.ncbi.nlm.nih.gov/pubmed/33116169
http://dx.doi.org/10.1038/s41598-020-74575-y
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