Cargando…
Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study
INTRODUCTION: Miswak is a tooth brushing stick that contains many ingredients that are beneficial for maintenance of oral hygiene. The aim of this study is to formulating a film containing miswak extract for treatment of chronic periodontitis. MATERIALS AND METHODS: Here we tested the miswak raw ext...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595484/ https://www.ncbi.nlm.nih.gov/pubmed/33149456 http://dx.doi.org/10.4103/jpbs.JPBS_59_20 |
_version_ | 1783601885533437952 |
---|---|
author | Sekar, Santhosh Jacob, Siji Suthanthiran, Thanga Kumaran Dhasthaheer, Syed Vikraman, Swathigan Kaliappan, Kanimozhi |
author_facet | Sekar, Santhosh Jacob, Siji Suthanthiran, Thanga Kumaran Dhasthaheer, Syed Vikraman, Swathigan Kaliappan, Kanimozhi |
author_sort | Sekar, Santhosh |
collection | PubMed |
description | INTRODUCTION: Miswak is a tooth brushing stick that contains many ingredients that are beneficial for maintenance of oral hygiene. The aim of this study is to formulating a film containing miswak extract for treatment of chronic periodontitis. MATERIALS AND METHODS: Here we tested the miswak raw extract invitro against Porphyromonas gingivalis and Herpes simplex virus-1 (HSV-1). The film was prepared using solvent casting method. Disintegration test was conducted to find the stability of the film. RESULTS: Cytotoxicity of miswak was also tested. Minimum inhibitory concentration against P. gingivalis was 62.5 µg/mL. Therapuetic index against HSV-1 was 11.3 µg/mL. Cytotoxicity against 50% Vero cells was present at 210 µg/mL. Based on this invitro study 100 µg/mL dose was calculated to be incorporated in a film of size 0.5 mm × 0.4 mm. This film is made of polymers HPMC K 100 and Eudragit L 100. Disintegration test of the film showed that they remained stable for around 5 days. CONCLUSION: In the present study we formulated the miswak raw extract containing film that can act against P. gingivalis and HSV-1. So it can be used to treat chronic periodontitis by placing it in periodontal pockets. |
format | Online Article Text |
id | pubmed-7595484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-75954842020-11-03 Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study Sekar, Santhosh Jacob, Siji Suthanthiran, Thanga Kumaran Dhasthaheer, Syed Vikraman, Swathigan Kaliappan, Kanimozhi J Pharm Bioallied Sci Original Article INTRODUCTION: Miswak is a tooth brushing stick that contains many ingredients that are beneficial for maintenance of oral hygiene. The aim of this study is to formulating a film containing miswak extract for treatment of chronic periodontitis. MATERIALS AND METHODS: Here we tested the miswak raw extract invitro against Porphyromonas gingivalis and Herpes simplex virus-1 (HSV-1). The film was prepared using solvent casting method. Disintegration test was conducted to find the stability of the film. RESULTS: Cytotoxicity of miswak was also tested. Minimum inhibitory concentration against P. gingivalis was 62.5 µg/mL. Therapuetic index against HSV-1 was 11.3 µg/mL. Cytotoxicity against 50% Vero cells was present at 210 µg/mL. Based on this invitro study 100 µg/mL dose was calculated to be incorporated in a film of size 0.5 mm × 0.4 mm. This film is made of polymers HPMC K 100 and Eudragit L 100. Disintegration test of the film showed that they remained stable for around 5 days. CONCLUSION: In the present study we formulated the miswak raw extract containing film that can act against P. gingivalis and HSV-1. So it can be used to treat chronic periodontitis by placing it in periodontal pockets. Wolters Kluwer - Medknow 2020-08 2020-08-28 /pmc/articles/PMC7595484/ /pubmed/33149456 http://dx.doi.org/10.4103/jpbs.JPBS_59_20 Text en Copyright: © 2020 Journal of Pharmacy and Bioallied Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Sekar, Santhosh Jacob, Siji Suthanthiran, Thanga Kumaran Dhasthaheer, Syed Vikraman, Swathigan Kaliappan, Kanimozhi Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study |
title | Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study |
title_full | Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study |
title_fullStr | Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study |
title_full_unstemmed | Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study |
title_short | Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study |
title_sort | characterization and formulation of miswak film for the treatment of chronic periodontitis: an in vitro study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595484/ https://www.ncbi.nlm.nih.gov/pubmed/33149456 http://dx.doi.org/10.4103/jpbs.JPBS_59_20 |
work_keys_str_mv | AT sekarsanthosh characterizationandformulationofmiswakfilmforthetreatmentofchronicperiodontitisaninvitrostudy AT jacobsiji characterizationandformulationofmiswakfilmforthetreatmentofchronicperiodontitisaninvitrostudy AT suthanthiranthangakumaran characterizationandformulationofmiswakfilmforthetreatmentofchronicperiodontitisaninvitrostudy AT dhasthaheersyed characterizationandformulationofmiswakfilmforthetreatmentofchronicperiodontitisaninvitrostudy AT vikramanswathigan characterizationandformulationofmiswakfilmforthetreatmentofchronicperiodontitisaninvitrostudy AT kaliappankanimozhi characterizationandformulationofmiswakfilmforthetreatmentofchronicperiodontitisaninvitrostudy |