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Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study

INTRODUCTION: Miswak is a tooth brushing stick that contains many ingredients that are beneficial for maintenance of oral hygiene. The aim of this study is to formulating a film containing miswak extract for treatment of chronic periodontitis. MATERIALS AND METHODS: Here we tested the miswak raw ext...

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Autores principales: Sekar, Santhosh, Jacob, Siji, Suthanthiran, Thanga Kumaran, Dhasthaheer, Syed, Vikraman, Swathigan, Kaliappan, Kanimozhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595484/
https://www.ncbi.nlm.nih.gov/pubmed/33149456
http://dx.doi.org/10.4103/jpbs.JPBS_59_20
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author Sekar, Santhosh
Jacob, Siji
Suthanthiran, Thanga Kumaran
Dhasthaheer, Syed
Vikraman, Swathigan
Kaliappan, Kanimozhi
author_facet Sekar, Santhosh
Jacob, Siji
Suthanthiran, Thanga Kumaran
Dhasthaheer, Syed
Vikraman, Swathigan
Kaliappan, Kanimozhi
author_sort Sekar, Santhosh
collection PubMed
description INTRODUCTION: Miswak is a tooth brushing stick that contains many ingredients that are beneficial for maintenance of oral hygiene. The aim of this study is to formulating a film containing miswak extract for treatment of chronic periodontitis. MATERIALS AND METHODS: Here we tested the miswak raw extract invitro against Porphyromonas gingivalis and Herpes simplex virus-1 (HSV-1). The film was prepared using solvent casting method. Disintegration test was conducted to find the stability of the film. RESULTS: Cytotoxicity of miswak was also tested. Minimum inhibitory concentration against P. gingivalis was 62.5 µg/mL. Therapuetic index against HSV-1 was 11.3 µg/mL. Cytotoxicity against 50% Vero cells was present at 210 µg/mL. Based on this invitro study 100 µg/mL dose was calculated to be incorporated in a film of size 0.5 mm × 0.4 mm. This film is made of polymers HPMC K 100 and Eudragit L 100. Disintegration test of the film showed that they remained stable for around 5 days. CONCLUSION: In the present study we formulated the miswak raw extract containing film that can act against P. gingivalis and HSV-1. So it can be used to treat chronic periodontitis by placing it in periodontal pockets.
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spelling pubmed-75954842020-11-03 Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study Sekar, Santhosh Jacob, Siji Suthanthiran, Thanga Kumaran Dhasthaheer, Syed Vikraman, Swathigan Kaliappan, Kanimozhi J Pharm Bioallied Sci Original Article INTRODUCTION: Miswak is a tooth brushing stick that contains many ingredients that are beneficial for maintenance of oral hygiene. The aim of this study is to formulating a film containing miswak extract for treatment of chronic periodontitis. MATERIALS AND METHODS: Here we tested the miswak raw extract invitro against Porphyromonas gingivalis and Herpes simplex virus-1 (HSV-1). The film was prepared using solvent casting method. Disintegration test was conducted to find the stability of the film. RESULTS: Cytotoxicity of miswak was also tested. Minimum inhibitory concentration against P. gingivalis was 62.5 µg/mL. Therapuetic index against HSV-1 was 11.3 µg/mL. Cytotoxicity against 50% Vero cells was present at 210 µg/mL. Based on this invitro study 100 µg/mL dose was calculated to be incorporated in a film of size 0.5 mm × 0.4 mm. This film is made of polymers HPMC K 100 and Eudragit L 100. Disintegration test of the film showed that they remained stable for around 5 days. CONCLUSION: In the present study we formulated the miswak raw extract containing film that can act against P. gingivalis and HSV-1. So it can be used to treat chronic periodontitis by placing it in periodontal pockets. Wolters Kluwer - Medknow 2020-08 2020-08-28 /pmc/articles/PMC7595484/ /pubmed/33149456 http://dx.doi.org/10.4103/jpbs.JPBS_59_20 Text en Copyright: © 2020 Journal of Pharmacy and Bioallied Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Sekar, Santhosh
Jacob, Siji
Suthanthiran, Thanga Kumaran
Dhasthaheer, Syed
Vikraman, Swathigan
Kaliappan, Kanimozhi
Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study
title Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study
title_full Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study
title_fullStr Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study
title_full_unstemmed Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study
title_short Characterization and Formulation of Miswak Film for the Treatment of Chronic Periodontitis: An In Vitro Study
title_sort characterization and formulation of miswak film for the treatment of chronic periodontitis: an in vitro study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595484/
https://www.ncbi.nlm.nih.gov/pubmed/33149456
http://dx.doi.org/10.4103/jpbs.JPBS_59_20
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