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Assessment of Quality of Platelet-Rich Plasma Produced With Desktop Centrifuge and Comparison With Standardized Commercially Available Platelet-rich plasma
AIMS: The aim of this study was to assess the quality of platelet-rich plasma (PRP) produced by in-house desktop centrifuge method and compare it with that of standardized commercial PRP. MATERIALS AND METHODS: REMI desktop centrifuge was used to prepare PRP and to compare with standardized commerci...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595503/ https://www.ncbi.nlm.nih.gov/pubmed/33149529 http://dx.doi.org/10.4103/jpbs.JPBS_163_20 |
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author | Srinivasan, V Ramesh Rekha, M Edsor, Effie Raja, S. Pradheep Kumar, T. Dinesh Kalaiselvan, S. |
author_facet | Srinivasan, V Ramesh Rekha, M Edsor, Effie Raja, S. Pradheep Kumar, T. Dinesh Kalaiselvan, S. |
author_sort | Srinivasan, V Ramesh |
collection | PubMed |
description | AIMS: The aim of this study was to assess the quality of platelet-rich plasma (PRP) produced by in-house desktop centrifuge method and compare it with that of standardized commercial PRP. MATERIALS AND METHODS: REMI desktop centrifuge was used to prepare PRP and to compare with standardized commercial PRP by calculating the quantity of platelets using Beckman Coulter cell counter in 10 PRP samples and assessing the morphological quality of platelets using JEOL JEM transmission electron microscope (TEM). STATISTICAL ANALYSIS USED: The t test for platelet count in desktop PRP with the test value of therapeutic PRP was 12.618. The P value was <0.001, which was significant statistically. The data followed a normal distribution in normal Q-Q plot for platelet count in desktop centrifuge. So the test samples were not much deviated. RESULTS: The platelet count was lesser than that of standardized commercial PRP. When viewed under JEOL JEM transmission electron microscope, the α granules in platelets were intact and the morphological quality of the PRP was good. CONCLUSIONS: With this study, we have determined that the morphological quality of PRP produced by the in-house desktop centrifuge method is comparable to that of standardized commercial PRP. Though the quantity of platelets was less than 1 million cells/μL, the clinical results were good with desired bone formation, thereby providing good avenue for further research. |
format | Online Article Text |
id | pubmed-7595503 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-75955032020-11-03 Assessment of Quality of Platelet-Rich Plasma Produced With Desktop Centrifuge and Comparison With Standardized Commercially Available Platelet-rich plasma Srinivasan, V Ramesh Rekha, M Edsor, Effie Raja, S. Pradheep Kumar, T. Dinesh Kalaiselvan, S. J Pharm Bioallied Sci Original Article AIMS: The aim of this study was to assess the quality of platelet-rich plasma (PRP) produced by in-house desktop centrifuge method and compare it with that of standardized commercial PRP. MATERIALS AND METHODS: REMI desktop centrifuge was used to prepare PRP and to compare with standardized commercial PRP by calculating the quantity of platelets using Beckman Coulter cell counter in 10 PRP samples and assessing the morphological quality of platelets using JEOL JEM transmission electron microscope (TEM). STATISTICAL ANALYSIS USED: The t test for platelet count in desktop PRP with the test value of therapeutic PRP was 12.618. The P value was <0.001, which was significant statistically. The data followed a normal distribution in normal Q-Q plot for platelet count in desktop centrifuge. So the test samples were not much deviated. RESULTS: The platelet count was lesser than that of standardized commercial PRP. When viewed under JEOL JEM transmission electron microscope, the α granules in platelets were intact and the morphological quality of the PRP was good. CONCLUSIONS: With this study, we have determined that the morphological quality of PRP produced by the in-house desktop centrifuge method is comparable to that of standardized commercial PRP. Though the quantity of platelets was less than 1 million cells/μL, the clinical results were good with desired bone formation, thereby providing good avenue for further research. Wolters Kluwer - Medknow 2020-08 2020-08-28 /pmc/articles/PMC7595503/ /pubmed/33149529 http://dx.doi.org/10.4103/jpbs.JPBS_163_20 Text en Copyright: © 2020 Journal of Pharmacy and Bioallied Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Srinivasan, V Ramesh Rekha, M Edsor, Effie Raja, S. Pradheep Kumar, T. Dinesh Kalaiselvan, S. Assessment of Quality of Platelet-Rich Plasma Produced With Desktop Centrifuge and Comparison With Standardized Commercially Available Platelet-rich plasma |
title | Assessment of Quality of Platelet-Rich Plasma Produced With Desktop Centrifuge and Comparison With Standardized Commercially Available Platelet-rich plasma |
title_full | Assessment of Quality of Platelet-Rich Plasma Produced With Desktop Centrifuge and Comparison With Standardized Commercially Available Platelet-rich plasma |
title_fullStr | Assessment of Quality of Platelet-Rich Plasma Produced With Desktop Centrifuge and Comparison With Standardized Commercially Available Platelet-rich plasma |
title_full_unstemmed | Assessment of Quality of Platelet-Rich Plasma Produced With Desktop Centrifuge and Comparison With Standardized Commercially Available Platelet-rich plasma |
title_short | Assessment of Quality of Platelet-Rich Plasma Produced With Desktop Centrifuge and Comparison With Standardized Commercially Available Platelet-rich plasma |
title_sort | assessment of quality of platelet-rich plasma produced with desktop centrifuge and comparison with standardized commercially available platelet-rich plasma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595503/ https://www.ncbi.nlm.nih.gov/pubmed/33149529 http://dx.doi.org/10.4103/jpbs.JPBS_163_20 |
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