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Evaluation of Effectiveness of Intracanal Medicaments on Viability of Stem Cells of Apical Papilla

INTRODUCTION: Regeneration, in the field of endodontics, is the process of restoring and maintaining both architectural form and biological functions of damaged tooth. Presently, regenerative endodontics is not hypothetical and is an alternative to conventional apexification procedures. There is a d...

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Autores principales: Jain, Gaurav, Goel, Akriti, Rajkumar, Balakrishnan, Bedi, Ravinder Singh, Bharti, Divya, Sawardeker, Anuradha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595550/
https://www.ncbi.nlm.nih.gov/pubmed/33149462
http://dx.doi.org/10.4103/jpbs.JPBS_67_20
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author Jain, Gaurav
Goel, Akriti
Rajkumar, Balakrishnan
Bedi, Ravinder Singh
Bharti, Divya
Sawardeker, Anuradha
author_facet Jain, Gaurav
Goel, Akriti
Rajkumar, Balakrishnan
Bedi, Ravinder Singh
Bharti, Divya
Sawardeker, Anuradha
author_sort Jain, Gaurav
collection PubMed
description INTRODUCTION: Regeneration, in the field of endodontics, is the process of restoring and maintaining both architectural form and biological functions of damaged tooth. Presently, regenerative endodontics is not hypothetical and is an alternative to conventional apexification procedures. There is a deficient knowledge concerning the role of intracanal medicaments and their effect on dental stem cells. AIM: The aim of this study was to evaluate the effectiveness of commonly used intracanal medicaments on the viability of dental stem cells of the apical papilla (SCAPs). MATERIALS AND METHODS: SCAPs were cultured and subjected to various concentrations including triple antibiotic paste, double antibiotic paste, Augmentin, and calcium hydroxide (Ca(OH)(2)). Viable percentage of stem cell counts was obtained 3 days after treatment. RESULTS: All three antibiotics used hereby significantly decreased SCAP cell survival at particular concentrations, whereas Ca(OH)(2) showed stimulating effect on SCAP survival. CONCLUSION: As per results obtained within limitations of this study, use of Ca(OH)(2) in regenerative endodontics in comparison to different commonly used antimicrobial combinations is recommended. Hereby, for clinical use, we suggest adequate concentrations of antimicrobials with adequate antibacterial efficacy should be used.
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spelling pubmed-75955502020-11-03 Evaluation of Effectiveness of Intracanal Medicaments on Viability of Stem Cells of Apical Papilla Jain, Gaurav Goel, Akriti Rajkumar, Balakrishnan Bedi, Ravinder Singh Bharti, Divya Sawardeker, Anuradha J Pharm Bioallied Sci Original Article INTRODUCTION: Regeneration, in the field of endodontics, is the process of restoring and maintaining both architectural form and biological functions of damaged tooth. Presently, regenerative endodontics is not hypothetical and is an alternative to conventional apexification procedures. There is a deficient knowledge concerning the role of intracanal medicaments and their effect on dental stem cells. AIM: The aim of this study was to evaluate the effectiveness of commonly used intracanal medicaments on the viability of dental stem cells of the apical papilla (SCAPs). MATERIALS AND METHODS: SCAPs were cultured and subjected to various concentrations including triple antibiotic paste, double antibiotic paste, Augmentin, and calcium hydroxide (Ca(OH)(2)). Viable percentage of stem cell counts was obtained 3 days after treatment. RESULTS: All three antibiotics used hereby significantly decreased SCAP cell survival at particular concentrations, whereas Ca(OH)(2) showed stimulating effect on SCAP survival. CONCLUSION: As per results obtained within limitations of this study, use of Ca(OH)(2) in regenerative endodontics in comparison to different commonly used antimicrobial combinations is recommended. Hereby, for clinical use, we suggest adequate concentrations of antimicrobials with adequate antibacterial efficacy should be used. Wolters Kluwer - Medknow 2020-08 2020-08-28 /pmc/articles/PMC7595550/ /pubmed/33149462 http://dx.doi.org/10.4103/jpbs.JPBS_67_20 Text en Copyright: © 2020 Journal of Pharmacy and Bioallied Sciences http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Jain, Gaurav
Goel, Akriti
Rajkumar, Balakrishnan
Bedi, Ravinder Singh
Bharti, Divya
Sawardeker, Anuradha
Evaluation of Effectiveness of Intracanal Medicaments on Viability of Stem Cells of Apical Papilla
title Evaluation of Effectiveness of Intracanal Medicaments on Viability of Stem Cells of Apical Papilla
title_full Evaluation of Effectiveness of Intracanal Medicaments on Viability of Stem Cells of Apical Papilla
title_fullStr Evaluation of Effectiveness of Intracanal Medicaments on Viability of Stem Cells of Apical Papilla
title_full_unstemmed Evaluation of Effectiveness of Intracanal Medicaments on Viability of Stem Cells of Apical Papilla
title_short Evaluation of Effectiveness of Intracanal Medicaments on Viability of Stem Cells of Apical Papilla
title_sort evaluation of effectiveness of intracanal medicaments on viability of stem cells of apical papilla
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595550/
https://www.ncbi.nlm.nih.gov/pubmed/33149462
http://dx.doi.org/10.4103/jpbs.JPBS_67_20
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