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Rotational manipulation of a microscopic object inside a microfluidic channel

Observations and analyses of a microscopic object are essential processes in various fields such as chemical engineering and life science. Microfluidic techniques with various functions and extensions have often been used for such purposes to investigate the mechanical properties of microscopic obje...

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Detalles Bibliográficos
Autores principales: Harada, Hiroyuki, Kaneko, Makoto, Ito, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595745/
https://www.ncbi.nlm.nih.gov/pubmed/33163134
http://dx.doi.org/10.1063/5.0013309
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author Harada, Hiroyuki
Kaneko, Makoto
Ito, Hiroaki
author_facet Harada, Hiroyuki
Kaneko, Makoto
Ito, Hiroaki
author_sort Harada, Hiroyuki
collection PubMed
description Observations and analyses of a microscopic object are essential processes in various fields such as chemical engineering and life science. Microfluidic techniques with various functions and extensions have often been used for such purposes to investigate the mechanical properties of microscopic objects such as biological cells. One of such extensions proposed in this context is a real-time visual feedback manipulation system, which is composed of a high-speed camera and a piezoelectric actuator with a single-line microfluidic channel. Although the on-chip manipulation system enables us to control the 1 degree-of-freedom position of a target object by the real-time pressure control, it has suffered from unintended changes in the object orientation, which is out of control in the previous system. In this study, we propose and demonstrate a novel shear-flow-based mechanism for the control of the orientation of a target object in addition to the position control in a microchannel to overcome the problem of the unintended rotation. We designed a tributary channel using a three-dimensional hydrodynamic simulation with boundary conditions appropriate for the particle manipulation to apply shear stress to the target particle placed at the junction and succeeded in rotating the particle at an angular velocity of 0.2 rad/s even under the position control in the experiment. The proposed mechanism would be applied to feedback controls of a target object in a microchannel to be in a desired orientation and at a desired position, which could be a universally useful function for various microfluidic platforms.
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spelling pubmed-75957452020-11-05 Rotational manipulation of a microscopic object inside a microfluidic channel Harada, Hiroyuki Kaneko, Makoto Ito, Hiroaki Biomicrofluidics Regular Articles Observations and analyses of a microscopic object are essential processes in various fields such as chemical engineering and life science. Microfluidic techniques with various functions and extensions have often been used for such purposes to investigate the mechanical properties of microscopic objects such as biological cells. One of such extensions proposed in this context is a real-time visual feedback manipulation system, which is composed of a high-speed camera and a piezoelectric actuator with a single-line microfluidic channel. Although the on-chip manipulation system enables us to control the 1 degree-of-freedom position of a target object by the real-time pressure control, it has suffered from unintended changes in the object orientation, which is out of control in the previous system. In this study, we propose and demonstrate a novel shear-flow-based mechanism for the control of the orientation of a target object in addition to the position control in a microchannel to overcome the problem of the unintended rotation. We designed a tributary channel using a three-dimensional hydrodynamic simulation with boundary conditions appropriate for the particle manipulation to apply shear stress to the target particle placed at the junction and succeeded in rotating the particle at an angular velocity of 0.2 rad/s even under the position control in the experiment. The proposed mechanism would be applied to feedback controls of a target object in a microchannel to be in a desired orientation and at a desired position, which could be a universally useful function for various microfluidic platforms. AIP Publishing LLC 2020-10-27 /pmc/articles/PMC7595745/ /pubmed/33163134 http://dx.doi.org/10.1063/5.0013309 Text en © 2020 Author(s). 1932-1058/2020/14(5)/054106/9 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Regular Articles
Harada, Hiroyuki
Kaneko, Makoto
Ito, Hiroaki
Rotational manipulation of a microscopic object inside a microfluidic channel
title Rotational manipulation of a microscopic object inside a microfluidic channel
title_full Rotational manipulation of a microscopic object inside a microfluidic channel
title_fullStr Rotational manipulation of a microscopic object inside a microfluidic channel
title_full_unstemmed Rotational manipulation of a microscopic object inside a microfluidic channel
title_short Rotational manipulation of a microscopic object inside a microfluidic channel
title_sort rotational manipulation of a microscopic object inside a microfluidic channel
topic Regular Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595745/
https://www.ncbi.nlm.nih.gov/pubmed/33163134
http://dx.doi.org/10.1063/5.0013309
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