WWP1在慢性淋巴细胞白血病中的异常表达及临床意义

OBJECTIVE: This study aims to investigate the expression of E3 ubiquitin-ligase（WWP1）in chronic lymphocytic leukemia（CLL）patients and analyze its correlation with clinical prognostic indicators（TP53, CD38, IGHV mutation）and its prognostic value. METHODS: A total of 48 CLL patients and 9 age-matched normal subjects were enrolled in the study. The WWP1 expression was detected by SYBR Green-based real-time PCR, and the clinical relationship was analyzed by GraphPad Prism software. RESULTS: The WWP1 median expression was 0.007（95％ CI 0.005–0.010）in the normal control group and 0.031（95％ CI 0.019–0.044）in the CLL group（P<0.001）. A sub-groups analysis implicated a statistically significant result（P＝0.022）, showing that the median time from a relatively high and low transcription level of WWP1 to the first treatment was 24 months and 35 months, respectively. Positive CD38 and ZAP-70 expressions were associated with a higher WWP1 expression（P＝0.012 and 0.029, respectively）. CONCLUSION: An abnormal WWP1 mRNA expression was found in CLL patients with significant correlation with ZAP-70 and CD38 expressions, and WWP1 may become a new supplement of CLL prognostic markers．