A novel fungal gene regulation system based on inducible VPR-dCas9 and nucleosome map-guided sgRNA positioning

ABSTRACT: Programmable transcriptional regulation is a powerful tool to study gene functions. Current methods to selectively regulate target genes are mainly based on promoter exchange or on overexpressing transcriptional activators. To expand the discovery toolbox, we designed a dCas9-based RNA-gui...

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Detalles Bibliográficos
Autores principales: Schüller, Andreas, Wolansky, Lisa, Berger, Harald, Studt, Lena, Gacek-Matthews, Agnieszka, Sulyok, Michael, Strauss, Joseph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Methods and Protocols
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595996/
https://www.ncbi.nlm.nih.gov/pubmed/33006690
http://dx.doi.org/10.1007/s00253-020-10900-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7595996/
https://www.ncbi.nlm.nih.gov/pubmed/33006690
http://dx.doi.org/10.1007/s00253-020-10900-9

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