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Contribution of Podoviridae and Myoviridae bacteriophages to the effectiveness of anti-staphylococcal therapeutic cocktails

Bacteriophage therapy is considered one of the most promising therapeutic approaches against multi-drug resistant bacterial infections. Infections caused by Staphylococcus aureus are very efficiently controlled with therapeutic bacteriophage cocktails, containing a number of individual phages infect...

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Autores principales: Kornienko, Maria, Kuptsov, Nikita, Gorodnichev, Roman, Bespiatykh, Dmitry, Guliaev, Andrei, Letarova, Maria, Kulikov, Eugene, Veselovsky, Vladimir, Malakhova, Maya, Letarov, Andrey, Ilina, Elena, Shitikov, Egor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596081/
https://www.ncbi.nlm.nih.gov/pubmed/33122703
http://dx.doi.org/10.1038/s41598-020-75637-x
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author Kornienko, Maria
Kuptsov, Nikita
Gorodnichev, Roman
Bespiatykh, Dmitry
Guliaev, Andrei
Letarova, Maria
Kulikov, Eugene
Veselovsky, Vladimir
Malakhova, Maya
Letarov, Andrey
Ilina, Elena
Shitikov, Egor
author_facet Kornienko, Maria
Kuptsov, Nikita
Gorodnichev, Roman
Bespiatykh, Dmitry
Guliaev, Andrei
Letarova, Maria
Kulikov, Eugene
Veselovsky, Vladimir
Malakhova, Maya
Letarov, Andrey
Ilina, Elena
Shitikov, Egor
author_sort Kornienko, Maria
collection PubMed
description Bacteriophage therapy is considered one of the most promising therapeutic approaches against multi-drug resistant bacterial infections. Infections caused by Staphylococcus aureus are very efficiently controlled with therapeutic bacteriophage cocktails, containing a number of individual phages infecting a majority of known pathogenic S. aureus strains. We assessed the contribution of individual bacteriophages comprising a therapeutic bacteriophage cocktail against S. aureus in order to optimize its composition. Two lytic bacteriophages vB_SauM-515A1 (Myoviridae) and vB_SauP-436A (Podoviridae) were isolated from the commercial therapeutic cocktail produced by Microgen (Russia). Host ranges of the phages were established on the panel of 75 S. aureus strains. Phage vB_SauM-515A1 lysed 85.3% and vB_SauP-436A lysed 68.0% of the strains, however, vB_SauP-436A was active against four strains resistant to vB_SauM-515A1, as well as to the therapeutic cocktail per se. Suboptimal results of the therapeutic cocktail application were due to extremely low vB_SauP-436A1 content in this composition. Optimization of the phage titers led to an increase in overall cocktail efficiency. Thus, one of the effective ways to optimize the phage cocktails design was demonstrated and realized by using bacteriophages of different families and lytic spectra.
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spelling pubmed-75960812020-10-30 Contribution of Podoviridae and Myoviridae bacteriophages to the effectiveness of anti-staphylococcal therapeutic cocktails Kornienko, Maria Kuptsov, Nikita Gorodnichev, Roman Bespiatykh, Dmitry Guliaev, Andrei Letarova, Maria Kulikov, Eugene Veselovsky, Vladimir Malakhova, Maya Letarov, Andrey Ilina, Elena Shitikov, Egor Sci Rep Article Bacteriophage therapy is considered one of the most promising therapeutic approaches against multi-drug resistant bacterial infections. Infections caused by Staphylococcus aureus are very efficiently controlled with therapeutic bacteriophage cocktails, containing a number of individual phages infecting a majority of known pathogenic S. aureus strains. We assessed the contribution of individual bacteriophages comprising a therapeutic bacteriophage cocktail against S. aureus in order to optimize its composition. Two lytic bacteriophages vB_SauM-515A1 (Myoviridae) and vB_SauP-436A (Podoviridae) were isolated from the commercial therapeutic cocktail produced by Microgen (Russia). Host ranges of the phages were established on the panel of 75 S. aureus strains. Phage vB_SauM-515A1 lysed 85.3% and vB_SauP-436A lysed 68.0% of the strains, however, vB_SauP-436A was active against four strains resistant to vB_SauM-515A1, as well as to the therapeutic cocktail per se. Suboptimal results of the therapeutic cocktail application were due to extremely low vB_SauP-436A1 content in this composition. Optimization of the phage titers led to an increase in overall cocktail efficiency. Thus, one of the effective ways to optimize the phage cocktails design was demonstrated and realized by using bacteriophages of different families and lytic spectra. Nature Publishing Group UK 2020-10-29 /pmc/articles/PMC7596081/ /pubmed/33122703 http://dx.doi.org/10.1038/s41598-020-75637-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kornienko, Maria
Kuptsov, Nikita
Gorodnichev, Roman
Bespiatykh, Dmitry
Guliaev, Andrei
Letarova, Maria
Kulikov, Eugene
Veselovsky, Vladimir
Malakhova, Maya
Letarov, Andrey
Ilina, Elena
Shitikov, Egor
Contribution of Podoviridae and Myoviridae bacteriophages to the effectiveness of anti-staphylococcal therapeutic cocktails
title Contribution of Podoviridae and Myoviridae bacteriophages to the effectiveness of anti-staphylococcal therapeutic cocktails
title_full Contribution of Podoviridae and Myoviridae bacteriophages to the effectiveness of anti-staphylococcal therapeutic cocktails
title_fullStr Contribution of Podoviridae and Myoviridae bacteriophages to the effectiveness of anti-staphylococcal therapeutic cocktails
title_full_unstemmed Contribution of Podoviridae and Myoviridae bacteriophages to the effectiveness of anti-staphylococcal therapeutic cocktails
title_short Contribution of Podoviridae and Myoviridae bacteriophages to the effectiveness of anti-staphylococcal therapeutic cocktails
title_sort contribution of podoviridae and myoviridae bacteriophages to the effectiveness of anti-staphylococcal therapeutic cocktails
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596081/
https://www.ncbi.nlm.nih.gov/pubmed/33122703
http://dx.doi.org/10.1038/s41598-020-75637-x
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