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TaUGT6, a Novel UDP-Glycosyltransferase Gene Enhances the Resistance to FHB and DON Accumulation in Wheat
Fusarium head blight (FHB), a devastating wheat disease, results in loss of yield and production of mycotoxins including deoxynivalenol (DON) in infected grains. DON is harmful to human and animal health and facilitates the spread of FHB symptoms. Its conversion into DON-3-glucoside (D3G) by UDP-gly...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596251/ https://www.ncbi.nlm.nih.gov/pubmed/33178244 http://dx.doi.org/10.3389/fpls.2020.574775 |
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author | He, Yi Wu, Lei Liu, Xiang Jiang, Peng Yu, Lixuan Qiu, Jianbo Wang, Gang Zhang, Xu Ma, Hongxiang |
author_facet | He, Yi Wu, Lei Liu, Xiang Jiang, Peng Yu, Lixuan Qiu, Jianbo Wang, Gang Zhang, Xu Ma, Hongxiang |
author_sort | He, Yi |
collection | PubMed |
description | Fusarium head blight (FHB), a devastating wheat disease, results in loss of yield and production of mycotoxins including deoxynivalenol (DON) in infected grains. DON is harmful to human and animal health and facilitates the spread of FHB symptoms. Its conversion into DON-3-glucoside (D3G) by UDP-glycosyltransferases (UGTs) is correlated with FHB resistance, and only few gene members in wheat have been investigated. Here, Fusarium graminearum and DON-induced TaUGT6 expression in the resistant cultivar Sumai 3 was cloned and characterized. TaUGT6::GFP was subcellularly located throughout cells. Purified TaUGT6 protein could convert DON into D3G to some extent in vitro. Transformation of TaUGT6 into Arabidopsis increased root tolerance when grown on agar plates containing DON. Furthermore, TaUGT6 overexpression in wheat showed improved resistance to Fusarium spread after F. graminearum inoculation. Overall, this study provides useful insight into a novel UGT gene for FHB resistance in wheat. |
format | Online Article Text |
id | pubmed-7596251 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-75962512020-11-10 TaUGT6, a Novel UDP-Glycosyltransferase Gene Enhances the Resistance to FHB and DON Accumulation in Wheat He, Yi Wu, Lei Liu, Xiang Jiang, Peng Yu, Lixuan Qiu, Jianbo Wang, Gang Zhang, Xu Ma, Hongxiang Front Plant Sci Plant Science Fusarium head blight (FHB), a devastating wheat disease, results in loss of yield and production of mycotoxins including deoxynivalenol (DON) in infected grains. DON is harmful to human and animal health and facilitates the spread of FHB symptoms. Its conversion into DON-3-glucoside (D3G) by UDP-glycosyltransferases (UGTs) is correlated with FHB resistance, and only few gene members in wheat have been investigated. Here, Fusarium graminearum and DON-induced TaUGT6 expression in the resistant cultivar Sumai 3 was cloned and characterized. TaUGT6::GFP was subcellularly located throughout cells. Purified TaUGT6 protein could convert DON into D3G to some extent in vitro. Transformation of TaUGT6 into Arabidopsis increased root tolerance when grown on agar plates containing DON. Furthermore, TaUGT6 overexpression in wheat showed improved resistance to Fusarium spread after F. graminearum inoculation. Overall, this study provides useful insight into a novel UGT gene for FHB resistance in wheat. Frontiers Media S.A. 2020-10-16 /pmc/articles/PMC7596251/ /pubmed/33178244 http://dx.doi.org/10.3389/fpls.2020.574775 Text en Copyright © 2020 He, Wu, Liu, Jiang, Yu, Qiu, Wang, Zhang and Ma. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science He, Yi Wu, Lei Liu, Xiang Jiang, Peng Yu, Lixuan Qiu, Jianbo Wang, Gang Zhang, Xu Ma, Hongxiang TaUGT6, a Novel UDP-Glycosyltransferase Gene Enhances the Resistance to FHB and DON Accumulation in Wheat |
title | TaUGT6, a Novel UDP-Glycosyltransferase Gene Enhances the Resistance to FHB and DON Accumulation in Wheat |
title_full | TaUGT6, a Novel UDP-Glycosyltransferase Gene Enhances the Resistance to FHB and DON Accumulation in Wheat |
title_fullStr | TaUGT6, a Novel UDP-Glycosyltransferase Gene Enhances the Resistance to FHB and DON Accumulation in Wheat |
title_full_unstemmed | TaUGT6, a Novel UDP-Glycosyltransferase Gene Enhances the Resistance to FHB and DON Accumulation in Wheat |
title_short | TaUGT6, a Novel UDP-Glycosyltransferase Gene Enhances the Resistance to FHB and DON Accumulation in Wheat |
title_sort | taugt6, a novel udp-glycosyltransferase gene enhances the resistance to fhb and don accumulation in wheat |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596251/ https://www.ncbi.nlm.nih.gov/pubmed/33178244 http://dx.doi.org/10.3389/fpls.2020.574775 |
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