Cargando…

Differences in Tetracycline Antibiotic Resistance Genes and Microbial Community Structure During Aerobic Composting and Anaerobic Digestion

Antibiotics are widely added to swine forage and are the main reason for the environmental accumulation of antibiotic resistance genes (ARGs) in swine manure-dwelling microorganisms. Aerobic composting (AC) and anaerobic digestion (AD) are efficient methods for converting swine manure to bio-fertili...

Descripción completa

Detalles Bibliográficos
Autores principales: Luo, Luyun, Zhang, Chengjia, Zhang, Zhuo, Peng, Jing, Han, Yongqin, Wang, Pei, Kong, Xiaoting, Rizwan, Hamid Muhammad, Zhang, Deyong, Su, Pin, Liu, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596291/
https://www.ncbi.nlm.nih.gov/pubmed/33178166
http://dx.doi.org/10.3389/fmicb.2020.583995
_version_ 1783602078889803776
author Luo, Luyun
Zhang, Chengjia
Zhang, Zhuo
Peng, Jing
Han, Yongqin
Wang, Pei
Kong, Xiaoting
Rizwan, Hamid Muhammad
Zhang, Deyong
Su, Pin
Liu, Yong
author_facet Luo, Luyun
Zhang, Chengjia
Zhang, Zhuo
Peng, Jing
Han, Yongqin
Wang, Pei
Kong, Xiaoting
Rizwan, Hamid Muhammad
Zhang, Deyong
Su, Pin
Liu, Yong
author_sort Luo, Luyun
collection PubMed
description Antibiotics are widely added to swine forage and are the main reason for the environmental accumulation of antibiotic resistance genes (ARGs) in swine manure-dwelling microorganisms. Aerobic composting (AC) and anaerobic digestion (AD) are efficient methods for converting swine manure to bio-fertilizer while degrading residual antibiotics. However, the influence of these methods on ARG accumulation and the difference in their efficiency have rarely been investigated. In this study, we explored the variations in four tetracycline antibiotics (TCs) and their associated ARGs and in microbial communities after AC and AD treatment. After full-scale manure AC and AD, the four TCs were removed effectively. AD had a higher TC removal efficiency than AC and a slower rate of TC-associated ARG accumulation. In addition, the community structure was more stable in the AC and AD manures than in untreated manure, and the relationship among microbial species also evolved into competition from mutualism after both AC and AD treatment. It was also speculated that the genera Acholeplasma and Arthrobacter were the possible hosts of tetO, tetW, and tetQ; the shift in the prokaryotic community composition and the alleviation of selective pressure by TC degradation led to decreased relative abundance of ARGs in AD- and AC-treated manure.
format Online
Article
Text
id pubmed-7596291
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-75962912020-11-10 Differences in Tetracycline Antibiotic Resistance Genes and Microbial Community Structure During Aerobic Composting and Anaerobic Digestion Luo, Luyun Zhang, Chengjia Zhang, Zhuo Peng, Jing Han, Yongqin Wang, Pei Kong, Xiaoting Rizwan, Hamid Muhammad Zhang, Deyong Su, Pin Liu, Yong Front Microbiol Microbiology Antibiotics are widely added to swine forage and are the main reason for the environmental accumulation of antibiotic resistance genes (ARGs) in swine manure-dwelling microorganisms. Aerobic composting (AC) and anaerobic digestion (AD) are efficient methods for converting swine manure to bio-fertilizer while degrading residual antibiotics. However, the influence of these methods on ARG accumulation and the difference in their efficiency have rarely been investigated. In this study, we explored the variations in four tetracycline antibiotics (TCs) and their associated ARGs and in microbial communities after AC and AD treatment. After full-scale manure AC and AD, the four TCs were removed effectively. AD had a higher TC removal efficiency than AC and a slower rate of TC-associated ARG accumulation. In addition, the community structure was more stable in the AC and AD manures than in untreated manure, and the relationship among microbial species also evolved into competition from mutualism after both AC and AD treatment. It was also speculated that the genera Acholeplasma and Arthrobacter were the possible hosts of tetO, tetW, and tetQ; the shift in the prokaryotic community composition and the alleviation of selective pressure by TC degradation led to decreased relative abundance of ARGs in AD- and AC-treated manure. Frontiers Media S.A. 2020-10-16 /pmc/articles/PMC7596291/ /pubmed/33178166 http://dx.doi.org/10.3389/fmicb.2020.583995 Text en Copyright © 2020 Luo, Zhang, Zhang, Peng, Han, Wang, Kong, Rizwan, Zhang, Su and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Luo, Luyun
Zhang, Chengjia
Zhang, Zhuo
Peng, Jing
Han, Yongqin
Wang, Pei
Kong, Xiaoting
Rizwan, Hamid Muhammad
Zhang, Deyong
Su, Pin
Liu, Yong
Differences in Tetracycline Antibiotic Resistance Genes and Microbial Community Structure During Aerobic Composting and Anaerobic Digestion
title Differences in Tetracycline Antibiotic Resistance Genes and Microbial Community Structure During Aerobic Composting and Anaerobic Digestion
title_full Differences in Tetracycline Antibiotic Resistance Genes and Microbial Community Structure During Aerobic Composting and Anaerobic Digestion
title_fullStr Differences in Tetracycline Antibiotic Resistance Genes and Microbial Community Structure During Aerobic Composting and Anaerobic Digestion
title_full_unstemmed Differences in Tetracycline Antibiotic Resistance Genes and Microbial Community Structure During Aerobic Composting and Anaerobic Digestion
title_short Differences in Tetracycline Antibiotic Resistance Genes and Microbial Community Structure During Aerobic Composting and Anaerobic Digestion
title_sort differences in tetracycline antibiotic resistance genes and microbial community structure during aerobic composting and anaerobic digestion
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596291/
https://www.ncbi.nlm.nih.gov/pubmed/33178166
http://dx.doi.org/10.3389/fmicb.2020.583995
work_keys_str_mv AT luoluyun differencesintetracyclineantibioticresistancegenesandmicrobialcommunitystructureduringaerobiccompostingandanaerobicdigestion
AT zhangchengjia differencesintetracyclineantibioticresistancegenesandmicrobialcommunitystructureduringaerobiccompostingandanaerobicdigestion
AT zhangzhuo differencesintetracyclineantibioticresistancegenesandmicrobialcommunitystructureduringaerobiccompostingandanaerobicdigestion
AT pengjing differencesintetracyclineantibioticresistancegenesandmicrobialcommunitystructureduringaerobiccompostingandanaerobicdigestion
AT hanyongqin differencesintetracyclineantibioticresistancegenesandmicrobialcommunitystructureduringaerobiccompostingandanaerobicdigestion
AT wangpei differencesintetracyclineantibioticresistancegenesandmicrobialcommunitystructureduringaerobiccompostingandanaerobicdigestion
AT kongxiaoting differencesintetracyclineantibioticresistancegenesandmicrobialcommunitystructureduringaerobiccompostingandanaerobicdigestion
AT rizwanhamidmuhammad differencesintetracyclineantibioticresistancegenesandmicrobialcommunitystructureduringaerobiccompostingandanaerobicdigestion
AT zhangdeyong differencesintetracyclineantibioticresistancegenesandmicrobialcommunitystructureduringaerobiccompostingandanaerobicdigestion
AT supin differencesintetracyclineantibioticresistancegenesandmicrobialcommunitystructureduringaerobiccompostingandanaerobicdigestion
AT liuyong differencesintetracyclineantibioticresistancegenesandmicrobialcommunitystructureduringaerobiccompostingandanaerobicdigestion