Cargando…

Amelioration by Idesia polycarpa Maxim. var. vestita Diels. of Oleic Acid-Induced Nonalcoholic Fatty Liver in HepG2 Cells through Antioxidant and Modulation of Lipid Metabolism

Idesia polycarpa Maxim. var. vestita Diels (I. polycarpa) is well known as an edible oil plant which contains abundant linoleic acid and polyphenols. The objective of this study was to maximize the by-product of defatted fruit of I. polycarpa. We found that the fraction D of ethyl acetate extract (E...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Na, Sun, Yi-ran, He, Li-bo, Huang, Lei, Li, Ting-ting, Wang, Tao-yu, Tang, Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596479/
https://www.ncbi.nlm.nih.gov/pubmed/33144913
http://dx.doi.org/10.1155/2020/1208726
_version_ 1783602120581185536
author Li, Na
Sun, Yi-ran
He, Li-bo
Huang, Lei
Li, Ting-ting
Wang, Tao-yu
Tang, Lin
author_facet Li, Na
Sun, Yi-ran
He, Li-bo
Huang, Lei
Li, Ting-ting
Wang, Tao-yu
Tang, Lin
author_sort Li, Na
collection PubMed
description Idesia polycarpa Maxim. var. vestita Diels (I. polycarpa) is well known as an edible oil plant which contains abundant linoleic acid and polyphenols. The objective of this study was to maximize the by-product of defatted fruit of I. polycarpa. We found that the fraction D of ethyl acetate extract (EF-D) contained more polyphenols, which contribute to its strong antioxidant activity by antioxidant assays (DPPH, ABTS, and FRAP). Meanwhile, EF-D showed a significant lipid-lowering effect on oleic acid- (OA-) induced hepatic steatosis in HepG2 cells through enhancing antioxidant activity, reducing liver damage, and regulating lipid metabolism, antioxidant, and inflammation-related gene expression. The SOD and T-AOC levels significantly increased, but the levels of MDA, AST, and ALT decreased obviously when treated with EF-D. In general, EF-D improved the antioxidant enzyme activities and decreased the hepatic injury activities. Besides, treatment with EF-D for NAFLD influenced lipid metabolism and inflammation by activating PPARα which was associated with the increased expression of CPT1 and decreased expression of SCD, NF-κB, and IL-1. Moreover, EF-D improved the oxidative stress system through activation of the Nrf2 antioxidant signal pathways and upregulated its target genes of HO-1, NQO1, and GSTA2. The results highlighted the EF-D from the defatted fruit of I. polycarpa regarding lipid-lowering, proving it to be a potential drug resource of natural products for treating the nonalcoholic fatty liver disease (NAFLD).
format Online
Article
Text
id pubmed-7596479
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-75964792020-11-02 Amelioration by Idesia polycarpa Maxim. var. vestita Diels. of Oleic Acid-Induced Nonalcoholic Fatty Liver in HepG2 Cells through Antioxidant and Modulation of Lipid Metabolism Li, Na Sun, Yi-ran He, Li-bo Huang, Lei Li, Ting-ting Wang, Tao-yu Tang, Lin Oxid Med Cell Longev Research Article Idesia polycarpa Maxim. var. vestita Diels (I. polycarpa) is well known as an edible oil plant which contains abundant linoleic acid and polyphenols. The objective of this study was to maximize the by-product of defatted fruit of I. polycarpa. We found that the fraction D of ethyl acetate extract (EF-D) contained more polyphenols, which contribute to its strong antioxidant activity by antioxidant assays (DPPH, ABTS, and FRAP). Meanwhile, EF-D showed a significant lipid-lowering effect on oleic acid- (OA-) induced hepatic steatosis in HepG2 cells through enhancing antioxidant activity, reducing liver damage, and regulating lipid metabolism, antioxidant, and inflammation-related gene expression. The SOD and T-AOC levels significantly increased, but the levels of MDA, AST, and ALT decreased obviously when treated with EF-D. In general, EF-D improved the antioxidant enzyme activities and decreased the hepatic injury activities. Besides, treatment with EF-D for NAFLD influenced lipid metabolism and inflammation by activating PPARα which was associated with the increased expression of CPT1 and decreased expression of SCD, NF-κB, and IL-1. Moreover, EF-D improved the oxidative stress system through activation of the Nrf2 antioxidant signal pathways and upregulated its target genes of HO-1, NQO1, and GSTA2. The results highlighted the EF-D from the defatted fruit of I. polycarpa regarding lipid-lowering, proving it to be a potential drug resource of natural products for treating the nonalcoholic fatty liver disease (NAFLD). Hindawi 2020-10-20 /pmc/articles/PMC7596479/ /pubmed/33144913 http://dx.doi.org/10.1155/2020/1208726 Text en Copyright © 2020 Na Li et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Na
Sun, Yi-ran
He, Li-bo
Huang, Lei
Li, Ting-ting
Wang, Tao-yu
Tang, Lin
Amelioration by Idesia polycarpa Maxim. var. vestita Diels. of Oleic Acid-Induced Nonalcoholic Fatty Liver in HepG2 Cells through Antioxidant and Modulation of Lipid Metabolism
title Amelioration by Idesia polycarpa Maxim. var. vestita Diels. of Oleic Acid-Induced Nonalcoholic Fatty Liver in HepG2 Cells through Antioxidant and Modulation of Lipid Metabolism
title_full Amelioration by Idesia polycarpa Maxim. var. vestita Diels. of Oleic Acid-Induced Nonalcoholic Fatty Liver in HepG2 Cells through Antioxidant and Modulation of Lipid Metabolism
title_fullStr Amelioration by Idesia polycarpa Maxim. var. vestita Diels. of Oleic Acid-Induced Nonalcoholic Fatty Liver in HepG2 Cells through Antioxidant and Modulation of Lipid Metabolism
title_full_unstemmed Amelioration by Idesia polycarpa Maxim. var. vestita Diels. of Oleic Acid-Induced Nonalcoholic Fatty Liver in HepG2 Cells through Antioxidant and Modulation of Lipid Metabolism
title_short Amelioration by Idesia polycarpa Maxim. var. vestita Diels. of Oleic Acid-Induced Nonalcoholic Fatty Liver in HepG2 Cells through Antioxidant and Modulation of Lipid Metabolism
title_sort amelioration by idesia polycarpa maxim. var. vestita diels. of oleic acid-induced nonalcoholic fatty liver in hepg2 cells through antioxidant and modulation of lipid metabolism
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596479/
https://www.ncbi.nlm.nih.gov/pubmed/33144913
http://dx.doi.org/10.1155/2020/1208726
work_keys_str_mv AT lina ameliorationbyidesiapolycarpamaximvarvestitadielsofoleicacidinducednonalcoholicfattyliverinhepg2cellsthroughantioxidantandmodulationoflipidmetabolism
AT sunyiran ameliorationbyidesiapolycarpamaximvarvestitadielsofoleicacidinducednonalcoholicfattyliverinhepg2cellsthroughantioxidantandmodulationoflipidmetabolism
AT helibo ameliorationbyidesiapolycarpamaximvarvestitadielsofoleicacidinducednonalcoholicfattyliverinhepg2cellsthroughantioxidantandmodulationoflipidmetabolism
AT huanglei ameliorationbyidesiapolycarpamaximvarvestitadielsofoleicacidinducednonalcoholicfattyliverinhepg2cellsthroughantioxidantandmodulationoflipidmetabolism
AT litingting ameliorationbyidesiapolycarpamaximvarvestitadielsofoleicacidinducednonalcoholicfattyliverinhepg2cellsthroughantioxidantandmodulationoflipidmetabolism
AT wangtaoyu ameliorationbyidesiapolycarpamaximvarvestitadielsofoleicacidinducednonalcoholicfattyliverinhepg2cellsthroughantioxidantandmodulationoflipidmetabolism
AT tanglin ameliorationbyidesiapolycarpamaximvarvestitadielsofoleicacidinducednonalcoholicfattyliverinhepg2cellsthroughantioxidantandmodulationoflipidmetabolism