A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression

[Image: see text] A novel photodeactivation strategy for controlling gene expression has been developed based on light-induced activation of cAMP response element binding protein (CREB). Light-induced cleavage of the photoresponsive protecting group of an antagonist of CREB binding protein (CBP) res...

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Autores principales: Imoto, Takuma, Minoshima, Masafumi, Yokoyama, Tatsushi, Emery, Ben P., Bull, Steven D., Bito, Haruhiko, Kikuchi, Kazuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596873/
https://www.ncbi.nlm.nih.gov/pubmed/33145417
http://dx.doi.org/10.1021/acscentsci.0c00736
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author Imoto, Takuma
Minoshima, Masafumi
Yokoyama, Tatsushi
Emery, Ben P.
Bull, Steven D.
Bito, Haruhiko
Kikuchi, Kazuya
author_facet Imoto, Takuma
Minoshima, Masafumi
Yokoyama, Tatsushi
Emery, Ben P.
Bull, Steven D.
Bito, Haruhiko
Kikuchi, Kazuya
author_sort Imoto, Takuma
collection PubMed
description [Image: see text] A novel photodeactivation strategy for controlling gene expression has been developed based on light-induced activation of cAMP response element binding protein (CREB). Light-induced cleavage of the photoresponsive protecting group of an antagonist of CREB binding protein (CBP) results in photocleaved products with weak binding affinity for CBP. This photodissociation reaction enables protein–protein interactions between CBP and CREB that trigger the formation of a multiprotein transcription complex to turn gene expression “on”. This enables irradiation of antagonist-treated HEK293T cells to be used to trigger temporal recovery of CREB-dependent transcriptional activity and endogenous gene expression under photolytic control.
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spelling pubmed-75968732020-11-02 A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression Imoto, Takuma Minoshima, Masafumi Yokoyama, Tatsushi Emery, Ben P. Bull, Steven D. Bito, Haruhiko Kikuchi, Kazuya ACS Cent Sci [Image: see text] A novel photodeactivation strategy for controlling gene expression has been developed based on light-induced activation of cAMP response element binding protein (CREB). Light-induced cleavage of the photoresponsive protecting group of an antagonist of CREB binding protein (CBP) results in photocleaved products with weak binding affinity for CBP. This photodissociation reaction enables protein–protein interactions between CBP and CREB that trigger the formation of a multiprotein transcription complex to turn gene expression “on”. This enables irradiation of antagonist-treated HEK293T cells to be used to trigger temporal recovery of CREB-dependent transcriptional activity and endogenous gene expression under photolytic control. American Chemical Society 2020-08-31 2020-10-28 /pmc/articles/PMC7596873/ /pubmed/33145417 http://dx.doi.org/10.1021/acscentsci.0c00736 Text en This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Imoto, Takuma
Minoshima, Masafumi
Yokoyama, Tatsushi
Emery, Ben P.
Bull, Steven D.
Bito, Haruhiko
Kikuchi, Kazuya
A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression
title A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression
title_full A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression
title_fullStr A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression
title_full_unstemmed A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression
title_short A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression
title_sort photodeactivatable antagonist for controlling creb-dependent gene expression
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596873/
https://www.ncbi.nlm.nih.gov/pubmed/33145417
http://dx.doi.org/10.1021/acscentsci.0c00736
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