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Development and application of a colloidal gold test strip for the rapid detection of the infectious laryngotracheitis virus

Infectious laryngotracheitis disease is an acute, highly contagious viral disease seriously affecting poultry industry worldwide. In this study, a rapid and simple immune colloidal gold test strip for detecting infectious laryngotracheitis virus (ILTV) was developed based on membrane chromatography...

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Autores principales: Yu, Jifeng, Lin, Yi, Cao, Ye, Li, Xingyu, Liao, Dangjin, Ye, Yonggang, Pan, Meng, Ye, Jianqiang, Wei, Yong, Xiao, Lu, Tang, Junni, Kang, Runmin, Xie, Jin, Zhou, Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597402/
https://www.ncbi.nlm.nih.gov/pubmed/32359575
http://dx.doi.org/10.1016/j.psj.2019.11.066
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author Yu, Jifeng
Lin, Yi
Cao, Ye
Li, Xingyu
Liao, Dangjin
Ye, Yonggang
Pan, Meng
Ye, Jianqiang
Wei, Yong
Xiao, Lu
Tang, Junni
Kang, Runmin
Xie, Jin
Zhou, Long
author_facet Yu, Jifeng
Lin, Yi
Cao, Ye
Li, Xingyu
Liao, Dangjin
Ye, Yonggang
Pan, Meng
Ye, Jianqiang
Wei, Yong
Xiao, Lu
Tang, Junni
Kang, Runmin
Xie, Jin
Zhou, Long
author_sort Yu, Jifeng
collection PubMed
description Infectious laryngotracheitis disease is an acute, highly contagious viral disease seriously affecting poultry industry worldwide. In this study, a rapid and simple immune colloidal gold test strip for detecting infectious laryngotracheitis virus (ILTV) was developed based on membrane chromatography with monoclonal antibodies (mAbs) against gJ protein of ILTV and systematically evaluated for the detection of ILTV from clinical samples. mAb 2D4 1D7 was conjugated with colloidal gold as the detector antibody on the test strip. Another mAb, 1D8 1G3, was used as the capture complex at the test line (T-line), and goat antimouse IgG antibody was used as the capture antibody at the control line (C-line). The colloidal gold test strip showed high specificity in the detection of ILTV, with no cross-reaction with other avian pathogens, including infectious bronchitis virus, infectious bursal disease virus, avian influenza virus, Newcastle disease virus, fowl adenoviruses, and Marek's disease virus. Besides, the detection limit of this method was as low as 60 ELD(50)/mL for the ILTV Wanggang strain. Furthermore, we evaluated its application in 260 clinical samples suspected of infection with ILTV. Results from the strip test were nearly identical with those from real-time PCR (coincidence rate 99.6%) and showed higher sensitivity than conventional PCR. All the results obtained in this study indicated that the colloidal gold test strip can be applied as a simple, rapid, sensitive, and specific diagnostic tool for the detection of ILTV, especially in resource-limited areas.
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spelling pubmed-75974022020-11-02 Development and application of a colloidal gold test strip for the rapid detection of the infectious laryngotracheitis virus Yu, Jifeng Lin, Yi Cao, Ye Li, Xingyu Liao, Dangjin Ye, Yonggang Pan, Meng Ye, Jianqiang Wei, Yong Xiao, Lu Tang, Junni Kang, Runmin Xie, Jin Zhou, Long Poult Sci Immunology, Health and Disease Infectious laryngotracheitis disease is an acute, highly contagious viral disease seriously affecting poultry industry worldwide. In this study, a rapid and simple immune colloidal gold test strip for detecting infectious laryngotracheitis virus (ILTV) was developed based on membrane chromatography with monoclonal antibodies (mAbs) against gJ protein of ILTV and systematically evaluated for the detection of ILTV from clinical samples. mAb 2D4 1D7 was conjugated with colloidal gold as the detector antibody on the test strip. Another mAb, 1D8 1G3, was used as the capture complex at the test line (T-line), and goat antimouse IgG antibody was used as the capture antibody at the control line (C-line). The colloidal gold test strip showed high specificity in the detection of ILTV, with no cross-reaction with other avian pathogens, including infectious bronchitis virus, infectious bursal disease virus, avian influenza virus, Newcastle disease virus, fowl adenoviruses, and Marek's disease virus. Besides, the detection limit of this method was as low as 60 ELD(50)/mL for the ILTV Wanggang strain. Furthermore, we evaluated its application in 260 clinical samples suspected of infection with ILTV. Results from the strip test were nearly identical with those from real-time PCR (coincidence rate 99.6%) and showed higher sensitivity than conventional PCR. All the results obtained in this study indicated that the colloidal gold test strip can be applied as a simple, rapid, sensitive, and specific diagnostic tool for the detection of ILTV, especially in resource-limited areas. Elsevier 2020-03-17 /pmc/articles/PMC7597402/ /pubmed/32359575 http://dx.doi.org/10.1016/j.psj.2019.11.066 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Immunology, Health and Disease
Yu, Jifeng
Lin, Yi
Cao, Ye
Li, Xingyu
Liao, Dangjin
Ye, Yonggang
Pan, Meng
Ye, Jianqiang
Wei, Yong
Xiao, Lu
Tang, Junni
Kang, Runmin
Xie, Jin
Zhou, Long
Development and application of a colloidal gold test strip for the rapid detection of the infectious laryngotracheitis virus
title Development and application of a colloidal gold test strip for the rapid detection of the infectious laryngotracheitis virus
title_full Development and application of a colloidal gold test strip for the rapid detection of the infectious laryngotracheitis virus
title_fullStr Development and application of a colloidal gold test strip for the rapid detection of the infectious laryngotracheitis virus
title_full_unstemmed Development and application of a colloidal gold test strip for the rapid detection of the infectious laryngotracheitis virus
title_short Development and application of a colloidal gold test strip for the rapid detection of the infectious laryngotracheitis virus
title_sort development and application of a colloidal gold test strip for the rapid detection of the infectious laryngotracheitis virus
topic Immunology, Health and Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597402/
https://www.ncbi.nlm.nih.gov/pubmed/32359575
http://dx.doi.org/10.1016/j.psj.2019.11.066
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