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Assessing MHC-B diversity in Silkie chickens
The major histocompatibility complex (MHC) is a highly polymorphic region on chromosome 16, which contains numerous immune response genes, and is known to influence disease susceptibility and resistance in chickens. Variability of MHC-B haplotypes in various well-known and commercially utilized bree...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597446/ https://www.ncbi.nlm.nih.gov/pubmed/32359568 http://dx.doi.org/10.1016/j.psj.2020.01.005 |
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author | Tarrant, Katy J. Lopez, Rodrigo Loper, Meghan Fulton, Janet E. |
author_facet | Tarrant, Katy J. Lopez, Rodrigo Loper, Meghan Fulton, Janet E. |
author_sort | Tarrant, Katy J. |
collection | PubMed |
description | The major histocompatibility complex (MHC) is a highly polymorphic region on chromosome 16, which contains numerous immune response genes, and is known to influence disease susceptibility and resistance in chickens. Variability of MHC-B haplotypes in various well-known and commercially utilized breeds has previously been identified. This study aims to understand MHC-B diversity in the Silkie breed using a high-density SNP panel that encompasses the chicken MHC-B region. DNA was obtained from 74 females and 27 males from a commercial Silkie breeder colony that is maintained through minimal genetic selection practices. A previously described panel of 90 SNPs, all located within the MHC-B region, was used to evaluate MHC-B variability in the commercial Silkie breeder colony. MHC-B haplotypes identified from the individual SNP information in the Silkie colony were compared to published haplotypes from the same region. Of the 27 haplotypes identified in the Silkie population, 8 have been previously described. Nineteen haplotypes are unique to the Silkie population and include one novel recombinant and 2 additional possible novel recombinants. Six haplotypes were found at a frequency greater than 5% of the population, of which 4 are novel. Finally, Hardy Weinberg Equilibrium (HWE) was calculated for the observed haplotypes, which were found to be in HWE. This study shows considerable MHC-B diversity in the Silkie breed and adds further information on variability of the MHC-B region in the chicken. |
format | Online Article Text |
id | pubmed-7597446 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-75974462020-11-02 Assessing MHC-B diversity in Silkie chickens Tarrant, Katy J. Lopez, Rodrigo Loper, Meghan Fulton, Janet E. Poult Sci Genetics and Molecular Biology The major histocompatibility complex (MHC) is a highly polymorphic region on chromosome 16, which contains numerous immune response genes, and is known to influence disease susceptibility and resistance in chickens. Variability of MHC-B haplotypes in various well-known and commercially utilized breeds has previously been identified. This study aims to understand MHC-B diversity in the Silkie breed using a high-density SNP panel that encompasses the chicken MHC-B region. DNA was obtained from 74 females and 27 males from a commercial Silkie breeder colony that is maintained through minimal genetic selection practices. A previously described panel of 90 SNPs, all located within the MHC-B region, was used to evaluate MHC-B variability in the commercial Silkie breeder colony. MHC-B haplotypes identified from the individual SNP information in the Silkie colony were compared to published haplotypes from the same region. Of the 27 haplotypes identified in the Silkie population, 8 have been previously described. Nineteen haplotypes are unique to the Silkie population and include one novel recombinant and 2 additional possible novel recombinants. Six haplotypes were found at a frequency greater than 5% of the population, of which 4 are novel. Finally, Hardy Weinberg Equilibrium (HWE) was calculated for the observed haplotypes, which were found to be in HWE. This study shows considerable MHC-B diversity in the Silkie breed and adds further information on variability of the MHC-B region in the chicken. Elsevier 2020-03-19 /pmc/articles/PMC7597446/ /pubmed/32359568 http://dx.doi.org/10.1016/j.psj.2020.01.005 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Genetics and Molecular Biology Tarrant, Katy J. Lopez, Rodrigo Loper, Meghan Fulton, Janet E. Assessing MHC-B diversity in Silkie chickens |
title | Assessing MHC-B diversity in Silkie chickens |
title_full | Assessing MHC-B diversity in Silkie chickens |
title_fullStr | Assessing MHC-B diversity in Silkie chickens |
title_full_unstemmed | Assessing MHC-B diversity in Silkie chickens |
title_short | Assessing MHC-B diversity in Silkie chickens |
title_sort | assessing mhc-b diversity in silkie chickens |
topic | Genetics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597446/ https://www.ncbi.nlm.nih.gov/pubmed/32359568 http://dx.doi.org/10.1016/j.psj.2020.01.005 |
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