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Central H(2) histaminergic and alpha-2 adrenergic receptors involvement in crocetin-induced antinociception in orofacial formalin pain in rats

Previous findings have shown that saffron (Crocus sativus L.) extract and its active constituents produce antinociceptive effects in the rat models of orofacial pain. In the present study, the central H(2) histaminergic and alpha-2 adrenergic receptors involvement in crocetin-induced antinociception...

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Autores principales: Erfanparast, Amir, Tamaddonfard, Esmaeal, Henareh-Chareh, Farzin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Urmia University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597797/
https://www.ncbi.nlm.nih.gov/pubmed/33133459
http://dx.doi.org/10.30466/vrf.2018.83779.2101
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author Erfanparast, Amir
Tamaddonfard, Esmaeal
Henareh-Chareh, Farzin
author_facet Erfanparast, Amir
Tamaddonfard, Esmaeal
Henareh-Chareh, Farzin
author_sort Erfanparast, Amir
collection PubMed
description Previous findings have shown that saffron (Crocus sativus L.) extract and its active constituents produce antinociceptive effects in the rat models of orofacial pain. In the present study, the central H(2) histaminergic and alpha-2 adrenergic receptors involvement in crocetin-induced antinociception in orofacial formalin pain in rats was evaluated. The guide cannula was implanted into the fourth ventricle in ketamine-xylazine anesthetized rats. Subcutaneous injection of a diluted formalin solution (1.50%; 50.00 µL) into a vibrissae pad was used as a model of orofacial pain. Face rubbing behavior durations were recorded at 3 min blocks for 45 min. Formalin produced a biphasic pain response (first phase: 0-3 min and second phase: 15-33 min). Intra-fourth ventricle injections of crocetin (5.00 and 10.00 μg μL(-1)) suppressed, whereas yohimbine (10.00 μg μL(-1)) and naloxone (10.00 μg μL(-1)) increased the intensity of both phases of pain. Crocetin-induced antinociception was not prevented by central pretreatment with naloxone. However, the antinociceptive effect of crocetin (5.00 μg μL(-1)) was inhibited by prior administration of famotidine (10.00 μg μL(-1)) and yohimbine (10.00 μg μL(-1)). Our study showed that injection of crocetin into the cerebral fourth ventricle attenuated formalin-induced orofacial pain in rats. Central H(2) histaminergic and alpha-2 adrenergic receptors, but not opioid receptors, might be involved in crocetin-induced antinociception.
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spelling pubmed-75977972020-10-31 Central H(2) histaminergic and alpha-2 adrenergic receptors involvement in crocetin-induced antinociception in orofacial formalin pain in rats Erfanparast, Amir Tamaddonfard, Esmaeal Henareh-Chareh, Farzin Vet Res Forum Original Article Previous findings have shown that saffron (Crocus sativus L.) extract and its active constituents produce antinociceptive effects in the rat models of orofacial pain. In the present study, the central H(2) histaminergic and alpha-2 adrenergic receptors involvement in crocetin-induced antinociception in orofacial formalin pain in rats was evaluated. The guide cannula was implanted into the fourth ventricle in ketamine-xylazine anesthetized rats. Subcutaneous injection of a diluted formalin solution (1.50%; 50.00 µL) into a vibrissae pad was used as a model of orofacial pain. Face rubbing behavior durations were recorded at 3 min blocks for 45 min. Formalin produced a biphasic pain response (first phase: 0-3 min and second phase: 15-33 min). Intra-fourth ventricle injections of crocetin (5.00 and 10.00 μg μL(-1)) suppressed, whereas yohimbine (10.00 μg μL(-1)) and naloxone (10.00 μg μL(-1)) increased the intensity of both phases of pain. Crocetin-induced antinociception was not prevented by central pretreatment with naloxone. However, the antinociceptive effect of crocetin (5.00 μg μL(-1)) was inhibited by prior administration of famotidine (10.00 μg μL(-1)) and yohimbine (10.00 μg μL(-1)). Our study showed that injection of crocetin into the cerebral fourth ventricle attenuated formalin-induced orofacial pain in rats. Central H(2) histaminergic and alpha-2 adrenergic receptors, but not opioid receptors, might be involved in crocetin-induced antinociception. Urmia University Press 2020 2020-09-15 /pmc/articles/PMC7597797/ /pubmed/33133459 http://dx.doi.org/10.30466/vrf.2018.83779.2101 Text en © 2020 Urmia University. All rights reserved This is an open-access article distributed under the terms of the Creative Commons Attribution-noncommercial 4.0 International License, (https://creativecommons.org/licenses/by-nc/4.0/) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
Erfanparast, Amir
Tamaddonfard, Esmaeal
Henareh-Chareh, Farzin
Central H(2) histaminergic and alpha-2 adrenergic receptors involvement in crocetin-induced antinociception in orofacial formalin pain in rats
title Central H(2) histaminergic and alpha-2 adrenergic receptors involvement in crocetin-induced antinociception in orofacial formalin pain in rats
title_full Central H(2) histaminergic and alpha-2 adrenergic receptors involvement in crocetin-induced antinociception in orofacial formalin pain in rats
title_fullStr Central H(2) histaminergic and alpha-2 adrenergic receptors involvement in crocetin-induced antinociception in orofacial formalin pain in rats
title_full_unstemmed Central H(2) histaminergic and alpha-2 adrenergic receptors involvement in crocetin-induced antinociception in orofacial formalin pain in rats
title_short Central H(2) histaminergic and alpha-2 adrenergic receptors involvement in crocetin-induced antinociception in orofacial formalin pain in rats
title_sort central h(2) histaminergic and alpha-2 adrenergic receptors involvement in crocetin-induced antinociception in orofacial formalin pain in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597797/
https://www.ncbi.nlm.nih.gov/pubmed/33133459
http://dx.doi.org/10.30466/vrf.2018.83779.2101
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