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Effects of the DMRT1 genotype on the body weight and gut microbiota in the broiler chicken
Intestinal microbiota is a critical determinant of growth and risk of metabolic diseases. Our previous studies showed that the locus rs16775833 within the DMRT1 gene is significantly associated with variation in the population structure of the gut microbiota, which is involved in determining the BW...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597928/ https://www.ncbi.nlm.nih.gov/pubmed/32731992 http://dx.doi.org/10.1016/j.psj.2020.03.055 |
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author | Ji, Jian Xu, Yibin Luo, Chenglong He, Yanhua Xu, Xinchun Yan, Xia Li, Ying Shu, Dingming Qu, Hao |
author_facet | Ji, Jian Xu, Yibin Luo, Chenglong He, Yanhua Xu, Xinchun Yan, Xia Li, Ying Shu, Dingming Qu, Hao |
author_sort | Ji, Jian |
collection | PubMed |
description | Intestinal microbiota is a critical determinant of growth and risk of metabolic diseases. Our previous studies showed that the locus rs16775833 within the DMRT1 gene is significantly associated with variation in the population structure of the gut microbiota, which is involved in determining the BW of the chicken. To assess the accuracy of correlation of rs16775833 located in the DMRT1 gene on microbial population and BW in birds, 2 genotypes GG and TT in the rs16775833 were identified in Chinese Yellow broiler breeders. We found that BW in the TT genotype group was significantly higher than in the GG genotype group at 7 and 13 wk of age in 777 female chickens. A full-length 16S rRNA sequencing approach was used to further evaluate the fecal bacterial composition of female broilers in 11 TT genotype chickens with high weight (HW-TT) and 11 GG genotype chickens with low weight (LW-GG) at 91 D of age. Partial least squares discriminant analysis revealed that the microbiota of the HW-TT and LW-GG females were clearly separated into 2 clusters. Furthermore, we identified 13 significantly different (P < 0.05) microbes at the genus level and 17 significantly different (P < 0.05) species between the HW-TT and LW-GG groups. Our data show that rs16775833 can modulate the microbial community structure and is associated with the BW of birds. To our knowledge, this is the first time that DMRT1 has been identified as a specific host factor, which is not only involved in sex determination but also has an effect on microbial function that might regulate animal growth. |
format | Online Article Text |
id | pubmed-7597928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-75979282020-11-03 Effects of the DMRT1 genotype on the body weight and gut microbiota in the broiler chicken Ji, Jian Xu, Yibin Luo, Chenglong He, Yanhua Xu, Xinchun Yan, Xia Li, Ying Shu, Dingming Qu, Hao Poult Sci Microbiology and Food Safety Intestinal microbiota is a critical determinant of growth and risk of metabolic diseases. Our previous studies showed that the locus rs16775833 within the DMRT1 gene is significantly associated with variation in the population structure of the gut microbiota, which is involved in determining the BW of the chicken. To assess the accuracy of correlation of rs16775833 located in the DMRT1 gene on microbial population and BW in birds, 2 genotypes GG and TT in the rs16775833 were identified in Chinese Yellow broiler breeders. We found that BW in the TT genotype group was significantly higher than in the GG genotype group at 7 and 13 wk of age in 777 female chickens. A full-length 16S rRNA sequencing approach was used to further evaluate the fecal bacterial composition of female broilers in 11 TT genotype chickens with high weight (HW-TT) and 11 GG genotype chickens with low weight (LW-GG) at 91 D of age. Partial least squares discriminant analysis revealed that the microbiota of the HW-TT and LW-GG females were clearly separated into 2 clusters. Furthermore, we identified 13 significantly different (P < 0.05) microbes at the genus level and 17 significantly different (P < 0.05) species between the HW-TT and LW-GG groups. Our data show that rs16775833 can modulate the microbial community structure and is associated with the BW of birds. To our knowledge, this is the first time that DMRT1 has been identified as a specific host factor, which is not only involved in sex determination but also has an effect on microbial function that might regulate animal growth. Elsevier 2020-05-04 /pmc/articles/PMC7597928/ /pubmed/32731992 http://dx.doi.org/10.1016/j.psj.2020.03.055 Text en © 2020 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Microbiology and Food Safety Ji, Jian Xu, Yibin Luo, Chenglong He, Yanhua Xu, Xinchun Yan, Xia Li, Ying Shu, Dingming Qu, Hao Effects of the DMRT1 genotype on the body weight and gut microbiota in the broiler chicken |
title | Effects of the DMRT1 genotype on the body weight and gut microbiota in the broiler chicken |
title_full | Effects of the DMRT1 genotype on the body weight and gut microbiota in the broiler chicken |
title_fullStr | Effects of the DMRT1 genotype on the body weight and gut microbiota in the broiler chicken |
title_full_unstemmed | Effects of the DMRT1 genotype on the body weight and gut microbiota in the broiler chicken |
title_short | Effects of the DMRT1 genotype on the body weight and gut microbiota in the broiler chicken |
title_sort | effects of the dmrt1 genotype on the body weight and gut microbiota in the broiler chicken |
topic | Microbiology and Food Safety |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597928/ https://www.ncbi.nlm.nih.gov/pubmed/32731992 http://dx.doi.org/10.1016/j.psj.2020.03.055 |
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