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Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus

Newcastle disease is a kind of avian infectious disease caused by Newcastle disease virus (NDV). The virulence of NDV is dependent mainly on the fusion (F) protein and hemagglutinin-neuraminidase (HN) protein. The genomes of 2 viruses, NDV-Blackbird and NDV-Dove, are 99.9% similar, while NDV-Blackbi...

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Autores principales: Yan, Chuanqi, Liu, Haijin, Jia, Yanqing, Prince-Theodore, Daguia-Wenam, Yang, Mengqing, Addoma Adam, Fathalrhman Eisa, Ren, Juan, Cao, Xuhong, Wang, Xinglong, Xiao, Sa, Zhang, Shuxia, Yang, Zengqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597932/
https://www.ncbi.nlm.nih.gov/pubmed/32616231
http://dx.doi.org/10.1016/j.psj.2020.04.014
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author Yan, Chuanqi
Liu, Haijin
Jia, Yanqing
Prince-Theodore, Daguia-Wenam
Yang, Mengqing
Addoma Adam, Fathalrhman Eisa
Ren, Juan
Cao, Xuhong
Wang, Xinglong
Xiao, Sa
Zhang, Shuxia
Yang, Zengqi
author_facet Yan, Chuanqi
Liu, Haijin
Jia, Yanqing
Prince-Theodore, Daguia-Wenam
Yang, Mengqing
Addoma Adam, Fathalrhman Eisa
Ren, Juan
Cao, Xuhong
Wang, Xinglong
Xiao, Sa
Zhang, Shuxia
Yang, Zengqi
author_sort Yan, Chuanqi
collection PubMed
description Newcastle disease is a kind of avian infectious disease caused by Newcastle disease virus (NDV). The virulence of NDV is dependent mainly on the fusion (F) protein and hemagglutinin-neuraminidase (HN) protein. The genomes of 2 viruses, NDV-Blackbird and NDV-Dove, are 99.9% similar, while NDV-Blackbird is a velogenic virus, and NDV-Dove is a lentogenic virus. Further analysis revealed that the F proteins of the 2 strains were identical, and only 5 amino acid sites on the HN proteins were inconsistent. Five different HN mutant plasmids were constructed and analyzed in this study. The results showed that the mutation F110L caused a significant increase in fusion-promotion activity caused by an increase in neuraminidase activity. Because of the increase in receptor-binding activity caused by G116R, there was a significant increase in fusion-promotion activity. The mutation G54S resulted in a slight decrease in the fusion-promotion activity caused by a slight decrease in receptor-binding activity. The slight increase in the fusion-promotion activity caused by A469V was associated with a significant increase in neuraminidase activity. Therefore, the amino acids L110 and R116 played a key role in determining the virulence difference between NDV-Blackbird and NDV-Dove, which could lay a foundation for illuminating the virulence differences of NDV strains, as well as the development of attenuated vaccines.
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spelling pubmed-75979322020-11-03 Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus Yan, Chuanqi Liu, Haijin Jia, Yanqing Prince-Theodore, Daguia-Wenam Yang, Mengqing Addoma Adam, Fathalrhman Eisa Ren, Juan Cao, Xuhong Wang, Xinglong Xiao, Sa Zhang, Shuxia Yang, Zengqi Poult Sci Genetics and Molecular Biology Newcastle disease is a kind of avian infectious disease caused by Newcastle disease virus (NDV). The virulence of NDV is dependent mainly on the fusion (F) protein and hemagglutinin-neuraminidase (HN) protein. The genomes of 2 viruses, NDV-Blackbird and NDV-Dove, are 99.9% similar, while NDV-Blackbird is a velogenic virus, and NDV-Dove is a lentogenic virus. Further analysis revealed that the F proteins of the 2 strains were identical, and only 5 amino acid sites on the HN proteins were inconsistent. Five different HN mutant plasmids were constructed and analyzed in this study. The results showed that the mutation F110L caused a significant increase in fusion-promotion activity caused by an increase in neuraminidase activity. Because of the increase in receptor-binding activity caused by G116R, there was a significant increase in fusion-promotion activity. The mutation G54S resulted in a slight decrease in the fusion-promotion activity caused by a slight decrease in receptor-binding activity. The slight increase in the fusion-promotion activity caused by A469V was associated with a significant increase in neuraminidase activity. Therefore, the amino acids L110 and R116 played a key role in determining the virulence difference between NDV-Blackbird and NDV-Dove, which could lay a foundation for illuminating the virulence differences of NDV strains, as well as the development of attenuated vaccines. Elsevier 2020-05-04 /pmc/articles/PMC7597932/ /pubmed/32616231 http://dx.doi.org/10.1016/j.psj.2020.04.014 Text en © 2020 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Genetics and Molecular Biology
Yan, Chuanqi
Liu, Haijin
Jia, Yanqing
Prince-Theodore, Daguia-Wenam
Yang, Mengqing
Addoma Adam, Fathalrhman Eisa
Ren, Juan
Cao, Xuhong
Wang, Xinglong
Xiao, Sa
Zhang, Shuxia
Yang, Zengqi
Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus
title Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus
title_full Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus
title_fullStr Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus
title_full_unstemmed Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus
title_short Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus
title_sort screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of newcastle disease virus
topic Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597932/
https://www.ncbi.nlm.nih.gov/pubmed/32616231
http://dx.doi.org/10.1016/j.psj.2020.04.014
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