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Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus
Newcastle disease is a kind of avian infectious disease caused by Newcastle disease virus (NDV). The virulence of NDV is dependent mainly on the fusion (F) protein and hemagglutinin-neuraminidase (HN) protein. The genomes of 2 viruses, NDV-Blackbird and NDV-Dove, are 99.9% similar, while NDV-Blackbi...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597932/ https://www.ncbi.nlm.nih.gov/pubmed/32616231 http://dx.doi.org/10.1016/j.psj.2020.04.014 |
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author | Yan, Chuanqi Liu, Haijin Jia, Yanqing Prince-Theodore, Daguia-Wenam Yang, Mengqing Addoma Adam, Fathalrhman Eisa Ren, Juan Cao, Xuhong Wang, Xinglong Xiao, Sa Zhang, Shuxia Yang, Zengqi |
author_facet | Yan, Chuanqi Liu, Haijin Jia, Yanqing Prince-Theodore, Daguia-Wenam Yang, Mengqing Addoma Adam, Fathalrhman Eisa Ren, Juan Cao, Xuhong Wang, Xinglong Xiao, Sa Zhang, Shuxia Yang, Zengqi |
author_sort | Yan, Chuanqi |
collection | PubMed |
description | Newcastle disease is a kind of avian infectious disease caused by Newcastle disease virus (NDV). The virulence of NDV is dependent mainly on the fusion (F) protein and hemagglutinin-neuraminidase (HN) protein. The genomes of 2 viruses, NDV-Blackbird and NDV-Dove, are 99.9% similar, while NDV-Blackbird is a velogenic virus, and NDV-Dove is a lentogenic virus. Further analysis revealed that the F proteins of the 2 strains were identical, and only 5 amino acid sites on the HN proteins were inconsistent. Five different HN mutant plasmids were constructed and analyzed in this study. The results showed that the mutation F110L caused a significant increase in fusion-promotion activity caused by an increase in neuraminidase activity. Because of the increase in receptor-binding activity caused by G116R, there was a significant increase in fusion-promotion activity. The mutation G54S resulted in a slight decrease in the fusion-promotion activity caused by a slight decrease in receptor-binding activity. The slight increase in the fusion-promotion activity caused by A469V was associated with a significant increase in neuraminidase activity. Therefore, the amino acids L110 and R116 played a key role in determining the virulence difference between NDV-Blackbird and NDV-Dove, which could lay a foundation for illuminating the virulence differences of NDV strains, as well as the development of attenuated vaccines. |
format | Online Article Text |
id | pubmed-7597932 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-75979322020-11-03 Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus Yan, Chuanqi Liu, Haijin Jia, Yanqing Prince-Theodore, Daguia-Wenam Yang, Mengqing Addoma Adam, Fathalrhman Eisa Ren, Juan Cao, Xuhong Wang, Xinglong Xiao, Sa Zhang, Shuxia Yang, Zengqi Poult Sci Genetics and Molecular Biology Newcastle disease is a kind of avian infectious disease caused by Newcastle disease virus (NDV). The virulence of NDV is dependent mainly on the fusion (F) protein and hemagglutinin-neuraminidase (HN) protein. The genomes of 2 viruses, NDV-Blackbird and NDV-Dove, are 99.9% similar, while NDV-Blackbird is a velogenic virus, and NDV-Dove is a lentogenic virus. Further analysis revealed that the F proteins of the 2 strains were identical, and only 5 amino acid sites on the HN proteins were inconsistent. Five different HN mutant plasmids were constructed and analyzed in this study. The results showed that the mutation F110L caused a significant increase in fusion-promotion activity caused by an increase in neuraminidase activity. Because of the increase in receptor-binding activity caused by G116R, there was a significant increase in fusion-promotion activity. The mutation G54S resulted in a slight decrease in the fusion-promotion activity caused by a slight decrease in receptor-binding activity. The slight increase in the fusion-promotion activity caused by A469V was associated with a significant increase in neuraminidase activity. Therefore, the amino acids L110 and R116 played a key role in determining the virulence difference between NDV-Blackbird and NDV-Dove, which could lay a foundation for illuminating the virulence differences of NDV strains, as well as the development of attenuated vaccines. Elsevier 2020-05-04 /pmc/articles/PMC7597932/ /pubmed/32616231 http://dx.doi.org/10.1016/j.psj.2020.04.014 Text en © 2020 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Genetics and Molecular Biology Yan, Chuanqi Liu, Haijin Jia, Yanqing Prince-Theodore, Daguia-Wenam Yang, Mengqing Addoma Adam, Fathalrhman Eisa Ren, Juan Cao, Xuhong Wang, Xinglong Xiao, Sa Zhang, Shuxia Yang, Zengqi Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus |
title | Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus |
title_full | Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus |
title_fullStr | Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus |
title_full_unstemmed | Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus |
title_short | Screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of Newcastle disease virus |
title_sort | screening and mechanistic study of key sites of the hemagglutinin-neuraminidase protein related to the virulence of newcastle disease virus |
topic | Genetics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7597932/ https://www.ncbi.nlm.nih.gov/pubmed/32616231 http://dx.doi.org/10.1016/j.psj.2020.04.014 |
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