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The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature
Reproductive failure associated with heat stress is a well-known phenomenon in poultry. High temperatures also induce various metabolic disturbances in many animals. Because the liver plays a central role in metabolism, the present study aimed to clarify the relationship between liver and reproducti...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598027/ https://www.ncbi.nlm.nih.gov/pubmed/32868003 http://dx.doi.org/10.1016/j.psj.2020.05.025 |
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author | Pu, Shaoxia Usuda, Kento Nagaoka, Kentaro Gore, Andrea Crews, David Watanabe, Gen |
author_facet | Pu, Shaoxia Usuda, Kento Nagaoka, Kentaro Gore, Andrea Crews, David Watanabe, Gen |
author_sort | Pu, Shaoxia |
collection | PubMed |
description | Reproductive failure associated with heat stress is a well-known phenomenon in poultry. High temperatures also induce various metabolic disturbances in many animals. Because the liver plays a central role in metabolism, the present study aimed to clarify the relationship between liver and reproduction in Japanese quails exposed to high temperatures. In the consecutive 20-D experimental period, quails were treated with 25°C (control) or 34°C (heat) from 12:00 to 16:00. Eggs were collected for hatching. On completion of the experimental period, quails were humanely euthanized for hormone analyses (e.g., serum and ovarian follicles). Serum metabolites were analyzed using GC/MS. Liver and ovary samples were collected for mRNA levels, histomorphology, and metabolic analysis. Ovary and oviduct weights significantly decreased after daily heat exposure. The number and weight of hierarchical follicles also decreased. Consequently, egg weight decreased. Although there was no difference in fertilization rate, chick birth weight significantly decreased in the heated group. Corticosterone and 17β-estradiol in the serum significantly increased in the heated group. Yolk corticosterone and 17β-estradiol concentration and content were higher in the heated group. Ovary sterologenic enzymes gene P450scc and estrogen receptor expression level increased. The FSH receptor decreased in heat-stressed quails. MetaboAnalyst analysis indicated that high temperature affects propanoate metabolism, beta-alanine metabolism, aspartate metabolism, and histidine metabolism. Triglyceride and cholesterol levels in the liver increased in the heated group. The heated group also had an increased mRNA expression of AGPAT5, apoptosis gene caspase3, and the immunocytokine genes IL-6 and TLR4. However, NF-κB gene expression decreased. These results suggest that high temperatures affect lipid metabolism and apoptosis and cause inflammation in the liver. High temperature induced ovarian dysfunction, which resulted in the decline of hierarchical follicle number and weight, egg weight, and chick birth weight. The increased level of 17β-estradiol suggests liver damage. Protecting liver function from damage may assist quails cope in summer. |
format | Online Article Text |
id | pubmed-7598027 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-75980272020-11-03 The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature Pu, Shaoxia Usuda, Kento Nagaoka, Kentaro Gore, Andrea Crews, David Watanabe, Gen Poult Sci Physiology and Reproduction Reproductive failure associated with heat stress is a well-known phenomenon in poultry. High temperatures also induce various metabolic disturbances in many animals. Because the liver plays a central role in metabolism, the present study aimed to clarify the relationship between liver and reproduction in Japanese quails exposed to high temperatures. In the consecutive 20-D experimental period, quails were treated with 25°C (control) or 34°C (heat) from 12:00 to 16:00. Eggs were collected for hatching. On completion of the experimental period, quails were humanely euthanized for hormone analyses (e.g., serum and ovarian follicles). Serum metabolites were analyzed using GC/MS. Liver and ovary samples were collected for mRNA levels, histomorphology, and metabolic analysis. Ovary and oviduct weights significantly decreased after daily heat exposure. The number and weight of hierarchical follicles also decreased. Consequently, egg weight decreased. Although there was no difference in fertilization rate, chick birth weight significantly decreased in the heated group. Corticosterone and 17β-estradiol in the serum significantly increased in the heated group. Yolk corticosterone and 17β-estradiol concentration and content were higher in the heated group. Ovary sterologenic enzymes gene P450scc and estrogen receptor expression level increased. The FSH receptor decreased in heat-stressed quails. MetaboAnalyst analysis indicated that high temperature affects propanoate metabolism, beta-alanine metabolism, aspartate metabolism, and histidine metabolism. Triglyceride and cholesterol levels in the liver increased in the heated group. The heated group also had an increased mRNA expression of AGPAT5, apoptosis gene caspase3, and the immunocytokine genes IL-6 and TLR4. However, NF-κB gene expression decreased. These results suggest that high temperatures affect lipid metabolism and apoptosis and cause inflammation in the liver. High temperature induced ovarian dysfunction, which resulted in the decline of hierarchical follicle number and weight, egg weight, and chick birth weight. The increased level of 17β-estradiol suggests liver damage. Protecting liver function from damage may assist quails cope in summer. Elsevier 2020-06-20 /pmc/articles/PMC7598027/ /pubmed/32868003 http://dx.doi.org/10.1016/j.psj.2020.05.025 Text en © 2020 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Physiology and Reproduction Pu, Shaoxia Usuda, Kento Nagaoka, Kentaro Gore, Andrea Crews, David Watanabe, Gen The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature |
title | The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature |
title_full | The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature |
title_fullStr | The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature |
title_full_unstemmed | The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature |
title_short | The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature |
title_sort | relation between liver damage and reproduction in female japanese quail (coturnix japonica) exposed to high ambient temperature |
topic | Physiology and Reproduction |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598027/ https://www.ncbi.nlm.nih.gov/pubmed/32868003 http://dx.doi.org/10.1016/j.psj.2020.05.025 |
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