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The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature

Reproductive failure associated with heat stress is a well-known phenomenon in poultry. High temperatures also induce various metabolic disturbances in many animals. Because the liver plays a central role in metabolism, the present study aimed to clarify the relationship between liver and reproducti...

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Autores principales: Pu, Shaoxia, Usuda, Kento, Nagaoka, Kentaro, Gore, Andrea, Crews, David, Watanabe, Gen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598027/
https://www.ncbi.nlm.nih.gov/pubmed/32868003
http://dx.doi.org/10.1016/j.psj.2020.05.025
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author Pu, Shaoxia
Usuda, Kento
Nagaoka, Kentaro
Gore, Andrea
Crews, David
Watanabe, Gen
author_facet Pu, Shaoxia
Usuda, Kento
Nagaoka, Kentaro
Gore, Andrea
Crews, David
Watanabe, Gen
author_sort Pu, Shaoxia
collection PubMed
description Reproductive failure associated with heat stress is a well-known phenomenon in poultry. High temperatures also induce various metabolic disturbances in many animals. Because the liver plays a central role in metabolism, the present study aimed to clarify the relationship between liver and reproduction in Japanese quails exposed to high temperatures. In the consecutive 20-D experimental period, quails were treated with 25°C (control) or 34°C (heat) from 12:00 to 16:00. Eggs were collected for hatching. On completion of the experimental period, quails were humanely euthanized for hormone analyses (e.g., serum and ovarian follicles). Serum metabolites were analyzed using GC/MS. Liver and ovary samples were collected for mRNA levels, histomorphology, and metabolic analysis. Ovary and oviduct weights significantly decreased after daily heat exposure. The number and weight of hierarchical follicles also decreased. Consequently, egg weight decreased. Although there was no difference in fertilization rate, chick birth weight significantly decreased in the heated group. Corticosterone and 17β-estradiol in the serum significantly increased in the heated group. Yolk corticosterone and 17β-estradiol concentration and content were higher in the heated group. Ovary sterologenic enzymes gene P450scc and estrogen receptor expression level increased. The FSH receptor decreased in heat-stressed quails. MetaboAnalyst analysis indicated that high temperature affects propanoate metabolism, beta-alanine metabolism, aspartate metabolism, and histidine metabolism. Triglyceride and cholesterol levels in the liver increased in the heated group. The heated group also had an increased mRNA expression of AGPAT5, apoptosis gene caspase3, and the immunocytokine genes IL-6 and TLR4. However, NF-κB gene expression decreased. These results suggest that high temperatures affect lipid metabolism and apoptosis and cause inflammation in the liver. High temperature induced ovarian dysfunction, which resulted in the decline of hierarchical follicle number and weight, egg weight, and chick birth weight. The increased level of 17β-estradiol suggests liver damage. Protecting liver function from damage may assist quails cope in summer.
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spelling pubmed-75980272020-11-03 The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature Pu, Shaoxia Usuda, Kento Nagaoka, Kentaro Gore, Andrea Crews, David Watanabe, Gen Poult Sci Physiology and Reproduction Reproductive failure associated with heat stress is a well-known phenomenon in poultry. High temperatures also induce various metabolic disturbances in many animals. Because the liver plays a central role in metabolism, the present study aimed to clarify the relationship between liver and reproduction in Japanese quails exposed to high temperatures. In the consecutive 20-D experimental period, quails were treated with 25°C (control) or 34°C (heat) from 12:00 to 16:00. Eggs were collected for hatching. On completion of the experimental period, quails were humanely euthanized for hormone analyses (e.g., serum and ovarian follicles). Serum metabolites were analyzed using GC/MS. Liver and ovary samples were collected for mRNA levels, histomorphology, and metabolic analysis. Ovary and oviduct weights significantly decreased after daily heat exposure. The number and weight of hierarchical follicles also decreased. Consequently, egg weight decreased. Although there was no difference in fertilization rate, chick birth weight significantly decreased in the heated group. Corticosterone and 17β-estradiol in the serum significantly increased in the heated group. Yolk corticosterone and 17β-estradiol concentration and content were higher in the heated group. Ovary sterologenic enzymes gene P450scc and estrogen receptor expression level increased. The FSH receptor decreased in heat-stressed quails. MetaboAnalyst analysis indicated that high temperature affects propanoate metabolism, beta-alanine metabolism, aspartate metabolism, and histidine metabolism. Triglyceride and cholesterol levels in the liver increased in the heated group. The heated group also had an increased mRNA expression of AGPAT5, apoptosis gene caspase3, and the immunocytokine genes IL-6 and TLR4. However, NF-κB gene expression decreased. These results suggest that high temperatures affect lipid metabolism and apoptosis and cause inflammation in the liver. High temperature induced ovarian dysfunction, which resulted in the decline of hierarchical follicle number and weight, egg weight, and chick birth weight. The increased level of 17β-estradiol suggests liver damage. Protecting liver function from damage may assist quails cope in summer. Elsevier 2020-06-20 /pmc/articles/PMC7598027/ /pubmed/32868003 http://dx.doi.org/10.1016/j.psj.2020.05.025 Text en © 2020 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Physiology and Reproduction
Pu, Shaoxia
Usuda, Kento
Nagaoka, Kentaro
Gore, Andrea
Crews, David
Watanabe, Gen
The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature
title The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature
title_full The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature
title_fullStr The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature
title_full_unstemmed The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature
title_short The relation between liver damage and reproduction in female Japanese quail (Coturnix japonica) exposed to high ambient temperature
title_sort relation between liver damage and reproduction in female japanese quail (coturnix japonica) exposed to high ambient temperature
topic Physiology and Reproduction
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598027/
https://www.ncbi.nlm.nih.gov/pubmed/32868003
http://dx.doi.org/10.1016/j.psj.2020.05.025
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