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No evidence for sylvatic cycles of chikungunya, dengue and Zika viruses in African green monkeys (Chlorocebus aethiops sabaeus) on St. Kitts, West Indies
BACKGROUND: Dengue, chikungunya and Zika viruses (DENV, CHIKV and ZIKV) are transmitted in sylvatic transmission cycles between non-human primates and forest (sylvan) mosquitoes in Africa and Asia. It remains unclear if sylvatic cycles exist or could establish themselves elsewhere and contribute to...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598228/ https://www.ncbi.nlm.nih.gov/pubmed/33126907 http://dx.doi.org/10.1186/s13071-020-04419-1 |
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author | Valentine, Matthew John Ciraola, Brenda Aliota, Matthew Thomas Vandenplas, Michel Marchi, Silvia Tenebray, Bernard Leparc-Goffart, Isabelle Gallagher, Christa Ann Beierschmitt, Amy Corey, Tatiana Dore, Kerry McAuliffe de Lamballerie, Xavier Wang, Chengming Murdock, Courtney Cuin Kelly, Patrick John |
author_facet | Valentine, Matthew John Ciraola, Brenda Aliota, Matthew Thomas Vandenplas, Michel Marchi, Silvia Tenebray, Bernard Leparc-Goffart, Isabelle Gallagher, Christa Ann Beierschmitt, Amy Corey, Tatiana Dore, Kerry McAuliffe de Lamballerie, Xavier Wang, Chengming Murdock, Courtney Cuin Kelly, Patrick John |
author_sort | Valentine, Matthew John |
collection | PubMed |
description | BACKGROUND: Dengue, chikungunya and Zika viruses (DENV, CHIKV and ZIKV) are transmitted in sylvatic transmission cycles between non-human primates and forest (sylvan) mosquitoes in Africa and Asia. It remains unclear if sylvatic cycles exist or could establish themselves elsewhere and contribute to the epidemiology of these diseases. The Caribbean island of St. Kitts has a large African green monkey (AGM) (Chlorocebus aethiops sabaeus) population and is therefore ideally suited to investigate sylvatic cycles. METHODS: We tested 858 AGM sera by ELISA and PRNT for virus-specific antibodies and collected and identified 9704 potential arbovirus vector mosquitoes. Mosquitoes were homogenized in 513 pools for testing by viral isolation in cell culture and by multiplex RT-qPCR after RNA extraction to detect the presence of DENV, CHIKV and ZIKVs. DNA was extracted from 122 visibly blood-fed individual mosquitoes and a polymorphic region of the hydroxymethylbilane synthase gene (HMBS) was amplified by PCR to determine if mosquitoes had fed on AGMs or humans. RESULTS: All of the AGMs were negative for DENV, CHIKV or ZIKV antibodies. However, one AGM did have evidence of an undifferentiated Flavivirus infection. Similarly, DENV, CHIKV and ZIKV were not detected in any of the mosquito pools by PCR or culture. AGMs were not the source of any of the mosquito blood meals. CONCLUSION: Sylvatic cycles involving AGMs and DENV, CHIKV and ZIKV do not currently exist on St. Kitts. [Image: see text] |
format | Online Article Text |
id | pubmed-7598228 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-75982282020-11-02 No evidence for sylvatic cycles of chikungunya, dengue and Zika viruses in African green monkeys (Chlorocebus aethiops sabaeus) on St. Kitts, West Indies Valentine, Matthew John Ciraola, Brenda Aliota, Matthew Thomas Vandenplas, Michel Marchi, Silvia Tenebray, Bernard Leparc-Goffart, Isabelle Gallagher, Christa Ann Beierschmitt, Amy Corey, Tatiana Dore, Kerry McAuliffe de Lamballerie, Xavier Wang, Chengming Murdock, Courtney Cuin Kelly, Patrick John Parasit Vectors Research BACKGROUND: Dengue, chikungunya and Zika viruses (DENV, CHIKV and ZIKV) are transmitted in sylvatic transmission cycles between non-human primates and forest (sylvan) mosquitoes in Africa and Asia. It remains unclear if sylvatic cycles exist or could establish themselves elsewhere and contribute to the epidemiology of these diseases. The Caribbean island of St. Kitts has a large African green monkey (AGM) (Chlorocebus aethiops sabaeus) population and is therefore ideally suited to investigate sylvatic cycles. METHODS: We tested 858 AGM sera by ELISA and PRNT for virus-specific antibodies and collected and identified 9704 potential arbovirus vector mosquitoes. Mosquitoes were homogenized in 513 pools for testing by viral isolation in cell culture and by multiplex RT-qPCR after RNA extraction to detect the presence of DENV, CHIKV and ZIKVs. DNA was extracted from 122 visibly blood-fed individual mosquitoes and a polymorphic region of the hydroxymethylbilane synthase gene (HMBS) was amplified by PCR to determine if mosquitoes had fed on AGMs or humans. RESULTS: All of the AGMs were negative for DENV, CHIKV or ZIKV antibodies. However, one AGM did have evidence of an undifferentiated Flavivirus infection. Similarly, DENV, CHIKV and ZIKV were not detected in any of the mosquito pools by PCR or culture. AGMs were not the source of any of the mosquito blood meals. CONCLUSION: Sylvatic cycles involving AGMs and DENV, CHIKV and ZIKV do not currently exist on St. Kitts. [Image: see text] BioMed Central 2020-10-30 /pmc/articles/PMC7598228/ /pubmed/33126907 http://dx.doi.org/10.1186/s13071-020-04419-1 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Valentine, Matthew John Ciraola, Brenda Aliota, Matthew Thomas Vandenplas, Michel Marchi, Silvia Tenebray, Bernard Leparc-Goffart, Isabelle Gallagher, Christa Ann Beierschmitt, Amy Corey, Tatiana Dore, Kerry McAuliffe de Lamballerie, Xavier Wang, Chengming Murdock, Courtney Cuin Kelly, Patrick John No evidence for sylvatic cycles of chikungunya, dengue and Zika viruses in African green monkeys (Chlorocebus aethiops sabaeus) on St. Kitts, West Indies |
title | No evidence for sylvatic cycles of chikungunya, dengue and Zika viruses in African green monkeys (Chlorocebus aethiops sabaeus) on St. Kitts, West Indies |
title_full | No evidence for sylvatic cycles of chikungunya, dengue and Zika viruses in African green monkeys (Chlorocebus aethiops sabaeus) on St. Kitts, West Indies |
title_fullStr | No evidence for sylvatic cycles of chikungunya, dengue and Zika viruses in African green monkeys (Chlorocebus aethiops sabaeus) on St. Kitts, West Indies |
title_full_unstemmed | No evidence for sylvatic cycles of chikungunya, dengue and Zika viruses in African green monkeys (Chlorocebus aethiops sabaeus) on St. Kitts, West Indies |
title_short | No evidence for sylvatic cycles of chikungunya, dengue and Zika viruses in African green monkeys (Chlorocebus aethiops sabaeus) on St. Kitts, West Indies |
title_sort | no evidence for sylvatic cycles of chikungunya, dengue and zika viruses in african green monkeys (chlorocebus aethiops sabaeus) on st. kitts, west indies |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598228/ https://www.ncbi.nlm.nih.gov/pubmed/33126907 http://dx.doi.org/10.1186/s13071-020-04419-1 |
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