Nanomaterials and Annelid Immunity: A Comparative Survey to Reveal the Common Stress and Defense Responses of Two Sentinel Species to Nanomaterials in the Environment

SIMPLE SUMMARY: Nanotechnology is a dynamically developing field producing large amounts of nanocompounds that are applied in industry, daily life, and health care. During production, use, and waste these materials could end up in water or soil. Large scale contaminations of our environment are a threat to public health. Pollution can have harmful effects on the immune system, as revealed by numerous studies in humans and other vertebrates. The relative simplicity of invertebrate immune functions offers potentially sensitive and accessible means of monitoring the effects and complex interactions of nanoparticles which ultimately affect host resistance. Among terrestrial and freshwater invertebrates, earthworms and leeches are the “keystone” species to evaluate the health of our ecosystems. In this review we compare the conserved stress and immune responses of these invertebrate model organisms toward nanoparticles. The obtained knowledge provides exciting insights into the conserved molecular and cellular mechanisms of nanomaterial-related toxicity in invertebrates and vertebrates. Understanding the unique characteristics of engineered nanoproducts and their interactions with biological systems in our environment is essential to the safe realization of these materials in novel biomedical applications. ABSTRACT: Earthworms and leeches are sentinel animals that represent the annelid phylum within terrestrial and freshwater ecosystems, respectively. One early stress signal in these organisms is related to innate immunity, but how nanomaterials affect it is poorly characterized. In this survey, we compare the latest literature on earthworm and leeches with examples of their molecular/cellular responses to inorganic (silver nanoparticles) and organic (carbon nanotubes) nanomaterials. A special focus is placed on the role of annelid immunocytes in the evolutionarily conserved antioxidant and immune mechanisms and protein corona formation and probable endocytosis pathways involved in nanomaterial uptake. Our summary helps to realize why these environmental sentinels are beneficial to study the potential detrimental effects of nanomaterials.