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Anti-Inflammatory Potential of Cow, Donkey and Goat Milk Extracellular Vesicles as Revealed by Metabolomic Profile

In recent years, extracellular vesicles (EVs), cell-derived micro and nano-sized structures enclosed in a double-layer membrane, have been in the spotlight for their high potential in diagnostic and therapeutic applications. Indeed, they act as signal mediators between cells and/or tissues through d...

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Autores principales: Mecocci, Samanta, Gevi, Federica, Pietrucci, Daniele, Cavinato, Luca, Luly, Francesco R., Pascucci, Luisa, Petrini, Stefano, Ascenzioni, Fiorentina, Zolla, Lello, Chillemi, Giovanni, Cappelli, Katia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598260/
https://www.ncbi.nlm.nih.gov/pubmed/32977543
http://dx.doi.org/10.3390/nu12102908
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author Mecocci, Samanta
Gevi, Federica
Pietrucci, Daniele
Cavinato, Luca
Luly, Francesco R.
Pascucci, Luisa
Petrini, Stefano
Ascenzioni, Fiorentina
Zolla, Lello
Chillemi, Giovanni
Cappelli, Katia
author_facet Mecocci, Samanta
Gevi, Federica
Pietrucci, Daniele
Cavinato, Luca
Luly, Francesco R.
Pascucci, Luisa
Petrini, Stefano
Ascenzioni, Fiorentina
Zolla, Lello
Chillemi, Giovanni
Cappelli, Katia
author_sort Mecocci, Samanta
collection PubMed
description In recent years, extracellular vesicles (EVs), cell-derived micro and nano-sized structures enclosed in a double-layer membrane, have been in the spotlight for their high potential in diagnostic and therapeutic applications. Indeed, they act as signal mediators between cells and/or tissues through different mechanisms involving their complex cargo and exert a number of biological effects depending upon EVs subtype and cell source. Being produced by almost all cell types, they are found in every biological fluid including milk. Milk EVs (MEVs) can enter the intestinal cells by endocytosis and protect their labile cargos against harsh conditions in the intestinal tract. In this study, we performed a metabolomic analysis of MEVs, from three different species (i.e., bovine, goat and donkey) by mass spectroscopy (MS) coupled with Ultrahigh-performance liquid chromatography (UHPLC). Metabolites, both common or specific of a species, were identified and enriched metabolic pathways were investigated, with the final aim to evaluate their anti-inflammatory and immunomodulatory properties in view of prospective applications as a nutraceutical in inflammatory conditions. In particular, metabolites transported by MEVs are involved in common pathways among the three species. These metabolites, such as arginine, asparagine, glutathione and lysine, show immunomodulating effects. Moreover, MEVs in goat milk showed a greater number of enriched metabolic pathways as compared to the other kinds of milk.
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spelling pubmed-75982602020-10-31 Anti-Inflammatory Potential of Cow, Donkey and Goat Milk Extracellular Vesicles as Revealed by Metabolomic Profile Mecocci, Samanta Gevi, Federica Pietrucci, Daniele Cavinato, Luca Luly, Francesco R. Pascucci, Luisa Petrini, Stefano Ascenzioni, Fiorentina Zolla, Lello Chillemi, Giovanni Cappelli, Katia Nutrients Article In recent years, extracellular vesicles (EVs), cell-derived micro and nano-sized structures enclosed in a double-layer membrane, have been in the spotlight for their high potential in diagnostic and therapeutic applications. Indeed, they act as signal mediators between cells and/or tissues through different mechanisms involving their complex cargo and exert a number of biological effects depending upon EVs subtype and cell source. Being produced by almost all cell types, they are found in every biological fluid including milk. Milk EVs (MEVs) can enter the intestinal cells by endocytosis and protect their labile cargos against harsh conditions in the intestinal tract. In this study, we performed a metabolomic analysis of MEVs, from three different species (i.e., bovine, goat and donkey) by mass spectroscopy (MS) coupled with Ultrahigh-performance liquid chromatography (UHPLC). Metabolites, both common or specific of a species, were identified and enriched metabolic pathways were investigated, with the final aim to evaluate their anti-inflammatory and immunomodulatory properties in view of prospective applications as a nutraceutical in inflammatory conditions. In particular, metabolites transported by MEVs are involved in common pathways among the three species. These metabolites, such as arginine, asparagine, glutathione and lysine, show immunomodulating effects. Moreover, MEVs in goat milk showed a greater number of enriched metabolic pathways as compared to the other kinds of milk. MDPI 2020-09-23 /pmc/articles/PMC7598260/ /pubmed/32977543 http://dx.doi.org/10.3390/nu12102908 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mecocci, Samanta
Gevi, Federica
Pietrucci, Daniele
Cavinato, Luca
Luly, Francesco R.
Pascucci, Luisa
Petrini, Stefano
Ascenzioni, Fiorentina
Zolla, Lello
Chillemi, Giovanni
Cappelli, Katia
Anti-Inflammatory Potential of Cow, Donkey and Goat Milk Extracellular Vesicles as Revealed by Metabolomic Profile
title Anti-Inflammatory Potential of Cow, Donkey and Goat Milk Extracellular Vesicles as Revealed by Metabolomic Profile
title_full Anti-Inflammatory Potential of Cow, Donkey and Goat Milk Extracellular Vesicles as Revealed by Metabolomic Profile
title_fullStr Anti-Inflammatory Potential of Cow, Donkey and Goat Milk Extracellular Vesicles as Revealed by Metabolomic Profile
title_full_unstemmed Anti-Inflammatory Potential of Cow, Donkey and Goat Milk Extracellular Vesicles as Revealed by Metabolomic Profile
title_short Anti-Inflammatory Potential of Cow, Donkey and Goat Milk Extracellular Vesicles as Revealed by Metabolomic Profile
title_sort anti-inflammatory potential of cow, donkey and goat milk extracellular vesicles as revealed by metabolomic profile
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598260/
https://www.ncbi.nlm.nih.gov/pubmed/32977543
http://dx.doi.org/10.3390/nu12102908
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