Cargando…
Antibacterial and antibiofilm activities of thyme oil against foodborne multiple antibiotics-resistant Enterococcus faecalis
The inhibitory and bactericidal activities of thyme oil against the foodborne multiple antibiotics-resistant Enterococcus faecalis biofilm were evaluated in this study. Gas chromatography-mass spectrometry revealed that more than 70% of the composition of thyme oil is thymol. Crystal violet staining...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598324/ https://www.ncbi.nlm.nih.gov/pubmed/32988551 http://dx.doi.org/10.1016/j.psj.2020.06.067 |
_version_ | 1783602573795655680 |
---|---|
author | Liu, Fang Jin, Panpan Gong, Hansheng Sun, Zhilan Du, Lihui Wang, Daoying |
author_facet | Liu, Fang Jin, Panpan Gong, Hansheng Sun, Zhilan Du, Lihui Wang, Daoying |
author_sort | Liu, Fang |
collection | PubMed |
description | The inhibitory and bactericidal activities of thyme oil against the foodborne multiple antibiotics-resistant Enterococcus faecalis biofilm were evaluated in this study. Gas chromatography-mass spectrometry revealed that more than 70% of the composition of thyme oil is thymol. Crystal violet staining assay showed that 128 and 256 μg/mL thyme oil significantly inhibited the biofilm formation of E. faecalis. The cell adherence of E. faecalis, as shown by its swimming and swarming motilities, was reduced by thyme oil. The exopolysaccharide (EPS) quantification assay showed that thyme oil inhibited the EPS synthesis in E. faecalis biofilms. The 3D-view observations through confocal laser scanning and scanning electron microscopy suggested that cell adherence and biofilm thickness were decreased in thyme oil–treated biofilms. Quantitative real-time analyses showed that the transcription of ebp and epa gene clusters, which were related to cell mobility and EPS production, was inhibited by thyme oil. Thus, thyme oil effectively inhibited the biofilm formation of E. faecalis by affecting cell adherence and EPS synthesis. Furthermore, 2,048 and 4,096 μg/mL thyme oil can effectively inactivate E. faecalis population in the mature E. faecalis biofilms by 5.75 and 7.20 log CFU/mL, respectively, after 30 min of treatment. Thus, thyme oil at different concentrations can be used as an effective antibiofilm or germicidal agent to control E. faecalis biofilms. |
format | Online Article Text |
id | pubmed-7598324 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-75983242020-11-03 Antibacterial and antibiofilm activities of thyme oil against foodborne multiple antibiotics-resistant Enterococcus faecalis Liu, Fang Jin, Panpan Gong, Hansheng Sun, Zhilan Du, Lihui Wang, Daoying Poult Sci Microbiology and Food Safety The inhibitory and bactericidal activities of thyme oil against the foodborne multiple antibiotics-resistant Enterococcus faecalis biofilm were evaluated in this study. Gas chromatography-mass spectrometry revealed that more than 70% of the composition of thyme oil is thymol. Crystal violet staining assay showed that 128 and 256 μg/mL thyme oil significantly inhibited the biofilm formation of E. faecalis. The cell adherence of E. faecalis, as shown by its swimming and swarming motilities, was reduced by thyme oil. The exopolysaccharide (EPS) quantification assay showed that thyme oil inhibited the EPS synthesis in E. faecalis biofilms. The 3D-view observations through confocal laser scanning and scanning electron microscopy suggested that cell adherence and biofilm thickness were decreased in thyme oil–treated biofilms. Quantitative real-time analyses showed that the transcription of ebp and epa gene clusters, which were related to cell mobility and EPS production, was inhibited by thyme oil. Thus, thyme oil effectively inhibited the biofilm formation of E. faecalis by affecting cell adherence and EPS synthesis. Furthermore, 2,048 and 4,096 μg/mL thyme oil can effectively inactivate E. faecalis population in the mature E. faecalis biofilms by 5.75 and 7.20 log CFU/mL, respectively, after 30 min of treatment. Thus, thyme oil at different concentrations can be used as an effective antibiofilm or germicidal agent to control E. faecalis biofilms. Elsevier 2020-07-24 /pmc/articles/PMC7598324/ /pubmed/32988551 http://dx.doi.org/10.1016/j.psj.2020.06.067 Text en © 2020 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Microbiology and Food Safety Liu, Fang Jin, Panpan Gong, Hansheng Sun, Zhilan Du, Lihui Wang, Daoying Antibacterial and antibiofilm activities of thyme oil against foodborne multiple antibiotics-resistant Enterococcus faecalis |
title | Antibacterial and antibiofilm activities of thyme oil against foodborne multiple antibiotics-resistant Enterococcus faecalis |
title_full | Antibacterial and antibiofilm activities of thyme oil against foodborne multiple antibiotics-resistant Enterococcus faecalis |
title_fullStr | Antibacterial and antibiofilm activities of thyme oil against foodborne multiple antibiotics-resistant Enterococcus faecalis |
title_full_unstemmed | Antibacterial and antibiofilm activities of thyme oil against foodborne multiple antibiotics-resistant Enterococcus faecalis |
title_short | Antibacterial and antibiofilm activities of thyme oil against foodborne multiple antibiotics-resistant Enterococcus faecalis |
title_sort | antibacterial and antibiofilm activities of thyme oil against foodborne multiple antibiotics-resistant enterococcus faecalis |
topic | Microbiology and Food Safety |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598324/ https://www.ncbi.nlm.nih.gov/pubmed/32988551 http://dx.doi.org/10.1016/j.psj.2020.06.067 |
work_keys_str_mv | AT liufang antibacterialandantibiofilmactivitiesofthymeoilagainstfoodbornemultipleantibioticsresistantenterococcusfaecalis AT jinpanpan antibacterialandantibiofilmactivitiesofthymeoilagainstfoodbornemultipleantibioticsresistantenterococcusfaecalis AT gonghansheng antibacterialandantibiofilmactivitiesofthymeoilagainstfoodbornemultipleantibioticsresistantenterococcusfaecalis AT sunzhilan antibacterialandantibiofilmactivitiesofthymeoilagainstfoodbornemultipleantibioticsresistantenterococcusfaecalis AT dulihui antibacterialandantibiofilmactivitiesofthymeoilagainstfoodbornemultipleantibioticsresistantenterococcusfaecalis AT wangdaoying antibacterialandantibiofilmactivitiesofthymeoilagainstfoodbornemultipleantibioticsresistantenterococcusfaecalis |