A preparative chiral separation of hydroxychloroquine using supercritical fluid chromatography

A robust supercritical chromatography (SFC) method using an Enantiocel C2–5 column was developed for the multigram separation of the enantiomers of hydroxychloroquine (HCQ), affirming its use as a scalable technology and ability to provide quantities of each enantiomer for clinical evaluation. The e...

Descripción completa

Detalles Bibliográficos
Autores principales: Wilson, Laura J., Mi, Charles, Kraml, Christina M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598365/
https://www.ncbi.nlm.nih.gov/pubmed/33166894
http://dx.doi.org/10.1016/j.chroma.2020.461661
_version_ 1783602586356547584
author Wilson, Laura J.
Mi, Charles
Kraml, Christina M.
author_facet Wilson, Laura J.
Mi, Charles
Kraml, Christina M.
author_sort Wilson, Laura J.
collection PubMed
description A robust supercritical chromatography (SFC) method using an Enantiocel C2–5 column was developed for the multigram separation of the enantiomers of hydroxychloroquine (HCQ), affirming its use as a scalable technology and ability to provide quantities of each enantiomer for clinical evaluation. The enantiomers of HCQ were collected on a gram scale with greater than 99% enantiomeric excess. The S and R enantiomer elution order was confirmed using optical rotation determinations with comparison to previously determined assignments.
format Online
Article
Text
id pubmed-7598365
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier B.V.
record_format MEDLINE/PubMed
spelling pubmed-75983652020-11-02 A preparative chiral separation of hydroxychloroquine using supercritical fluid chromatography Wilson, Laura J. Mi, Charles Kraml, Christina M. J Chromatogr A Short Communication A robust supercritical chromatography (SFC) method using an Enantiocel C2–5 column was developed for the multigram separation of the enantiomers of hydroxychloroquine (HCQ), affirming its use as a scalable technology and ability to provide quantities of each enantiomer for clinical evaluation. The enantiomers of HCQ were collected on a gram scale with greater than 99% enantiomeric excess. The S and R enantiomer elution order was confirmed using optical rotation determinations with comparison to previously determined assignments. Elsevier B.V. 2020-12-20 2020-10-29 /pmc/articles/PMC7598365/ /pubmed/33166894 http://dx.doi.org/10.1016/j.chroma.2020.461661 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Short Communication
Wilson, Laura J.
Mi, Charles
Kraml, Christina M.
A preparative chiral separation of hydroxychloroquine using supercritical fluid chromatography
title A preparative chiral separation of hydroxychloroquine using supercritical fluid chromatography
title_full A preparative chiral separation of hydroxychloroquine using supercritical fluid chromatography
title_fullStr A preparative chiral separation of hydroxychloroquine using supercritical fluid chromatography
title_full_unstemmed A preparative chiral separation of hydroxychloroquine using supercritical fluid chromatography
title_short A preparative chiral separation of hydroxychloroquine using supercritical fluid chromatography
title_sort preparative chiral separation of hydroxychloroquine using supercritical fluid chromatography
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598365/
https://www.ncbi.nlm.nih.gov/pubmed/33166894
http://dx.doi.org/10.1016/j.chroma.2020.461661
work_keys_str_mv AT wilsonlauraj apreparativechiralseparationofhydroxychloroquineusingsupercriticalfluidchromatography
AT micharles apreparativechiralseparationofhydroxychloroquineusingsupercriticalfluidchromatography
AT kramlchristinam apreparativechiralseparationofhydroxychloroquineusingsupercriticalfluidchromatography
AT wilsonlauraj preparativechiralseparationofhydroxychloroquineusingsupercriticalfluidchromatography
AT micharles preparativechiralseparationofhydroxychloroquineusingsupercriticalfluidchromatography
AT kramlchristinam preparativechiralseparationofhydroxychloroquineusingsupercriticalfluidchromatography

Ejemplares similares