Adjusting RT-qPCR conditions to avoid unspecific amplification in SARS-CoV-2 diagnosis

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in December 2019 and quickly spread around the world, forcing global health authorities to develop protocols for its diagnosis. Here we report dimer formation in the N2 primers–probe set (CDC 2019-nCoV Real-Time RT-PCR) used in the...

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Detalles Bibliográficos
Autores principales: Jaeger, Lauren Hubert, Nascimento, Thiago César, Rocha, Fabíola Dutra, Vilela, Fernanda Maria Pinto, Duque, Ana Paula do Nascimento, Silva, Lívia Mara, Riani, Lorena Rodrigues, Moreira, João Paulo, Chagas, Jéssica Mara de Assis, Pereira, Thamiris Vilela, Perches, Carmen Gomide Pinto, Watanabe, Aripuana Sakurada Aranha, Viccini, Lyderson Facio, Silvério, Marcelo Silva, Corrêa, José Otávio do Amaral, Pereira-Junior, Olavo dos Santos, Pittella, Frederico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. 2021
Materias:
Case Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598553/
https://www.ncbi.nlm.nih.gov/pubmed/33130201
http://dx.doi.org/10.1016/j.ijid.2020.10.079

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