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Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics

In recent years, the increase of bacteria antibiotic- resistance has been a severe problem for public health. A useful solution could be to join some phytochemicals naturally present in essential oils (EOs) to the existing antibiotics, with the aim to increase their efficacy in therapies. According...

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Autores principales: Rosato, Antonio, Sblano, Sabina, Salvagno, Lara, Carocci, Alessia, Clodoveo, Maria Lisa, Corbo, Filomena, Fracchiolla, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598638/
https://www.ncbi.nlm.nih.gov/pubmed/32987638
http://dx.doi.org/10.3390/antibiotics9100637
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author Rosato, Antonio
Sblano, Sabina
Salvagno, Lara
Carocci, Alessia
Clodoveo, Maria Lisa
Corbo, Filomena
Fracchiolla, Giuseppe
author_facet Rosato, Antonio
Sblano, Sabina
Salvagno, Lara
Carocci, Alessia
Clodoveo, Maria Lisa
Corbo, Filomena
Fracchiolla, Giuseppe
author_sort Rosato, Antonio
collection PubMed
description In recent years, the increase of bacteria antibiotic- resistance has been a severe problem for public health. A useful solution could be to join some phytochemicals naturally present in essential oils (EOs) to the existing antibiotics, with the aim to increase their efficacy in therapies. According to in vitro studies, EOs and their components could show such effects. Among them, we studied the activity of Cinnammonum zeylanicum, Mentha piperita, Origanum vulgare, and Thymus vulgaris EOs on bacterial biofilm and their synergism when used in association with some common antibiotics such as norfloxacin, oxacillin, and gentamicin. The chemical composition of EOs was determined using gas chromatography (GC) coupled with mass spectrometry (MS) techniques. The EOs drug efficacy was evaluated on four different strains of Gram-positive bacteria forming biofilms. The synergistic effects were tested through the chequerboard microdilution method. The association EOs-antibiotics showed a strong destruction of the biofilm growth of the four bacterial species considered. The interaction of norfloxacin with EOs was the most effective in all the tested combinations against the strains object of this study. These preliminary results suggest the formulation of a new generation of antimicrobial agents based on a combination of antimicrobial compounds with different origin.
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spelling pubmed-75986382020-10-31 Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics Rosato, Antonio Sblano, Sabina Salvagno, Lara Carocci, Alessia Clodoveo, Maria Lisa Corbo, Filomena Fracchiolla, Giuseppe Antibiotics (Basel) Article In recent years, the increase of bacteria antibiotic- resistance has been a severe problem for public health. A useful solution could be to join some phytochemicals naturally present in essential oils (EOs) to the existing antibiotics, with the aim to increase their efficacy in therapies. According to in vitro studies, EOs and their components could show such effects. Among them, we studied the activity of Cinnammonum zeylanicum, Mentha piperita, Origanum vulgare, and Thymus vulgaris EOs on bacterial biofilm and their synergism when used in association with some common antibiotics such as norfloxacin, oxacillin, and gentamicin. The chemical composition of EOs was determined using gas chromatography (GC) coupled with mass spectrometry (MS) techniques. The EOs drug efficacy was evaluated on four different strains of Gram-positive bacteria forming biofilms. The synergistic effects were tested through the chequerboard microdilution method. The association EOs-antibiotics showed a strong destruction of the biofilm growth of the four bacterial species considered. The interaction of norfloxacin with EOs was the most effective in all the tested combinations against the strains object of this study. These preliminary results suggest the formulation of a new generation of antimicrobial agents based on a combination of antimicrobial compounds with different origin. MDPI 2020-09-24 /pmc/articles/PMC7598638/ /pubmed/32987638 http://dx.doi.org/10.3390/antibiotics9100637 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rosato, Antonio
Sblano, Sabina
Salvagno, Lara
Carocci, Alessia
Clodoveo, Maria Lisa
Corbo, Filomena
Fracchiolla, Giuseppe
Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics
title Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics
title_full Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics
title_fullStr Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics
title_full_unstemmed Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics
title_short Anti-Biofilm Inhibitory Synergistic Effects of Combinations of Essential Oils and Antibiotics
title_sort anti-biofilm inhibitory synergistic effects of combinations of essential oils and antibiotics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7598638/
https://www.ncbi.nlm.nih.gov/pubmed/32987638
http://dx.doi.org/10.3390/antibiotics9100637
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