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Identification and Analysis of microRNAs in Chlorella sorokiniana Using High-Throughput Sequencing

Chlorella is a popular microalga with robust physiological and biochemical characteristics, which can be cultured under various conditions. The exploration of the small RNA content of Chlorella could improve strategies for the enhancement of metabolite production from this microalga. In this study,...

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Autores principales: Azaman, Siti Nor Ani, Satharasinghe, Dilan Amila, Tan, Sheau Wei, Nagao, Norio, Yusoff, Fatimah M., Yeap, Swee Keong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599482/
https://www.ncbi.nlm.nih.gov/pubmed/32992970
http://dx.doi.org/10.3390/genes11101131
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author Azaman, Siti Nor Ani
Satharasinghe, Dilan Amila
Tan, Sheau Wei
Nagao, Norio
Yusoff, Fatimah M.
Yeap, Swee Keong
author_facet Azaman, Siti Nor Ani
Satharasinghe, Dilan Amila
Tan, Sheau Wei
Nagao, Norio
Yusoff, Fatimah M.
Yeap, Swee Keong
author_sort Azaman, Siti Nor Ani
collection PubMed
description Chlorella is a popular microalga with robust physiological and biochemical characteristics, which can be cultured under various conditions. The exploration of the small RNA content of Chlorella could improve strategies for the enhancement of metabolite production from this microalga. In this study, stress was introduced to the Chlorella sorokiniana culture to produce high-value metabolites such as carotenoids and phenolic content. The small RNA transcriptome of C. sorokiniana was sequenced, focusing on microRNA (miRNA) content. From the analysis, 98 miRNAs were identified in cultures subjected to normal and stress conditions. The functional analysis result showed that the miRNA targets found were most often involved in the biosynthesis of secondary metabolites, followed by protein metabolism, cell cycle, and porphyrin and chlorophyll metabolism. Furthermore, the biosynthesis of secondary metabolites such as carotenoids, terpenoids, and lipids was found mostly in stress conditions. These results may help to improve our understanding of regulatory mechanisms of miRNA in the biological and metabolic process of Chlorella species. It is important and timely to determine the true potential of this microalga species and to support the potential for genetic engineering of microalgae as they receive increasing focus for their development as an alternative source of biofuel, food, and health supplements.
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spelling pubmed-75994822020-11-01 Identification and Analysis of microRNAs in Chlorella sorokiniana Using High-Throughput Sequencing Azaman, Siti Nor Ani Satharasinghe, Dilan Amila Tan, Sheau Wei Nagao, Norio Yusoff, Fatimah M. Yeap, Swee Keong Genes (Basel) Communication Chlorella is a popular microalga with robust physiological and biochemical characteristics, which can be cultured under various conditions. The exploration of the small RNA content of Chlorella could improve strategies for the enhancement of metabolite production from this microalga. In this study, stress was introduced to the Chlorella sorokiniana culture to produce high-value metabolites such as carotenoids and phenolic content. The small RNA transcriptome of C. sorokiniana was sequenced, focusing on microRNA (miRNA) content. From the analysis, 98 miRNAs were identified in cultures subjected to normal and stress conditions. The functional analysis result showed that the miRNA targets found were most often involved in the biosynthesis of secondary metabolites, followed by protein metabolism, cell cycle, and porphyrin and chlorophyll metabolism. Furthermore, the biosynthesis of secondary metabolites such as carotenoids, terpenoids, and lipids was found mostly in stress conditions. These results may help to improve our understanding of regulatory mechanisms of miRNA in the biological and metabolic process of Chlorella species. It is important and timely to determine the true potential of this microalga species and to support the potential for genetic engineering of microalgae as they receive increasing focus for their development as an alternative source of biofuel, food, and health supplements. MDPI 2020-09-25 /pmc/articles/PMC7599482/ /pubmed/32992970 http://dx.doi.org/10.3390/genes11101131 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Azaman, Siti Nor Ani
Satharasinghe, Dilan Amila
Tan, Sheau Wei
Nagao, Norio
Yusoff, Fatimah M.
Yeap, Swee Keong
Identification and Analysis of microRNAs in Chlorella sorokiniana Using High-Throughput Sequencing
title Identification and Analysis of microRNAs in Chlorella sorokiniana Using High-Throughput Sequencing
title_full Identification and Analysis of microRNAs in Chlorella sorokiniana Using High-Throughput Sequencing
title_fullStr Identification and Analysis of microRNAs in Chlorella sorokiniana Using High-Throughput Sequencing
title_full_unstemmed Identification and Analysis of microRNAs in Chlorella sorokiniana Using High-Throughput Sequencing
title_short Identification and Analysis of microRNAs in Chlorella sorokiniana Using High-Throughput Sequencing
title_sort identification and analysis of micrornas in chlorella sorokiniana using high-throughput sequencing
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599482/
https://www.ncbi.nlm.nih.gov/pubmed/32992970
http://dx.doi.org/10.3390/genes11101131
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