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Characterization of the Age-Dependent Changes in Antioxidant Defenses and Protein’s Sulfhydryl/Carbonyl Stress in Human Follicular Fluid

The oxidative stress, characterized by the imbalance between pro-oxidants and antioxidants molecules, seems to be involved in the pathogenesis of female subfertility. In particular, the presence of different markers of oxidative stress has been reported in human follicular fluid (FF) surrounding ooc...

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Autores principales: Luddi, Alice, Governini, Laura, Capaldo, Angela, Campanella, Giovanna, De Leo, Vincenzo, Piomboni, Paola, Morgante, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599528/
https://www.ncbi.nlm.nih.gov/pubmed/32998216
http://dx.doi.org/10.3390/antiox9100927
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author Luddi, Alice
Governini, Laura
Capaldo, Angela
Campanella, Giovanna
De Leo, Vincenzo
Piomboni, Paola
Morgante, Giuseppe
author_facet Luddi, Alice
Governini, Laura
Capaldo, Angela
Campanella, Giovanna
De Leo, Vincenzo
Piomboni, Paola
Morgante, Giuseppe
author_sort Luddi, Alice
collection PubMed
description The oxidative stress, characterized by the imbalance between pro-oxidants and antioxidants molecules, seems to be involved in the pathogenesis of female subfertility. In particular, the presence of different markers of oxidative stress has been reported in human follicular fluid (FF) surrounding oocytes. Based on its distinctive composition and on the close proximity to the oocyte, FF creates a unique microenvironment having a direct impact on oocyte quality, implantation, and early embryo development. An imbalance in reactive oxygen species (ROS) production in ovarian follicular fluid may have a negative effect on these processes and, as a consequence, on female fertility. Therefore, the aim of this study was to evaluate the redox state of the FF through various methodological approaches. By means of 2D-electrophoresis we demonstrated that the main structural changes occurring in the proteins of the follicular fluid of normovulatory women were correlated to the age of the patients and to the antioxidant defenses present in the FF. Measurement of these parameters could have clinical relevance, since the assessment of the oxidative stress rate may be helpful in evaluating in vitro fertilization potential.
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spelling pubmed-75995282020-11-01 Characterization of the Age-Dependent Changes in Antioxidant Defenses and Protein’s Sulfhydryl/Carbonyl Stress in Human Follicular Fluid Luddi, Alice Governini, Laura Capaldo, Angela Campanella, Giovanna De Leo, Vincenzo Piomboni, Paola Morgante, Giuseppe Antioxidants (Basel) Article The oxidative stress, characterized by the imbalance between pro-oxidants and antioxidants molecules, seems to be involved in the pathogenesis of female subfertility. In particular, the presence of different markers of oxidative stress has been reported in human follicular fluid (FF) surrounding oocytes. Based on its distinctive composition and on the close proximity to the oocyte, FF creates a unique microenvironment having a direct impact on oocyte quality, implantation, and early embryo development. An imbalance in reactive oxygen species (ROS) production in ovarian follicular fluid may have a negative effect on these processes and, as a consequence, on female fertility. Therefore, the aim of this study was to evaluate the redox state of the FF through various methodological approaches. By means of 2D-electrophoresis we demonstrated that the main structural changes occurring in the proteins of the follicular fluid of normovulatory women were correlated to the age of the patients and to the antioxidant defenses present in the FF. Measurement of these parameters could have clinical relevance, since the assessment of the oxidative stress rate may be helpful in evaluating in vitro fertilization potential. MDPI 2020-09-28 /pmc/articles/PMC7599528/ /pubmed/32998216 http://dx.doi.org/10.3390/antiox9100927 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luddi, Alice
Governini, Laura
Capaldo, Angela
Campanella, Giovanna
De Leo, Vincenzo
Piomboni, Paola
Morgante, Giuseppe
Characterization of the Age-Dependent Changes in Antioxidant Defenses and Protein’s Sulfhydryl/Carbonyl Stress in Human Follicular Fluid
title Characterization of the Age-Dependent Changes in Antioxidant Defenses and Protein’s Sulfhydryl/Carbonyl Stress in Human Follicular Fluid
title_full Characterization of the Age-Dependent Changes in Antioxidant Defenses and Protein’s Sulfhydryl/Carbonyl Stress in Human Follicular Fluid
title_fullStr Characterization of the Age-Dependent Changes in Antioxidant Defenses and Protein’s Sulfhydryl/Carbonyl Stress in Human Follicular Fluid
title_full_unstemmed Characterization of the Age-Dependent Changes in Antioxidant Defenses and Protein’s Sulfhydryl/Carbonyl Stress in Human Follicular Fluid
title_short Characterization of the Age-Dependent Changes in Antioxidant Defenses and Protein’s Sulfhydryl/Carbonyl Stress in Human Follicular Fluid
title_sort characterization of the age-dependent changes in antioxidant defenses and protein’s sulfhydryl/carbonyl stress in human follicular fluid
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599528/
https://www.ncbi.nlm.nih.gov/pubmed/32998216
http://dx.doi.org/10.3390/antiox9100927
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