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Effective Dispensing Methods for Loading Drugs Only to the Tip of DNA Microneedles

Here, we propose a novel and simple method to efficiently capture the diffusion of fluorescein isothiocyanate (FITC)-dextran from a biocompatible substance and load the drug only to the tip of DNA microneedles. A dispensing and suction method was chosen to fabricate the designed microneedles with ef...

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Autores principales: Bok, Moonjeong, Zhao, Zhi-Jun, Hwang, Soon Hyoung, Kang, Hyeok-Joong, Jeon, Sohee, Ko, Jiwoo, Jeong, Jiwon, Song, Young Seok, Lim, Eunju, Jeong, Jun-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599544/
https://www.ncbi.nlm.nih.gov/pubmed/33050428
http://dx.doi.org/10.3390/pharmaceutics12100954
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author Bok, Moonjeong
Zhao, Zhi-Jun
Hwang, Soon Hyoung
Kang, Hyeok-Joong
Jeon, Sohee
Ko, Jiwoo
Jeong, Jiwon
Song, Young Seok
Lim, Eunju
Jeong, Jun-Ho
author_facet Bok, Moonjeong
Zhao, Zhi-Jun
Hwang, Soon Hyoung
Kang, Hyeok-Joong
Jeon, Sohee
Ko, Jiwoo
Jeong, Jiwon
Song, Young Seok
Lim, Eunju
Jeong, Jun-Ho
author_sort Bok, Moonjeong
collection PubMed
description Here, we propose a novel and simple method to efficiently capture the diffusion of fluorescein isothiocyanate (FITC)-dextran from a biocompatible substance and load the drug only to the tip of DNA microneedles. A dispensing and suction method was chosen to fabricate the designed microneedles with efficient amounts of FITC as the drug model. Importantly, the vacuum process, which could influence the capturing of FITC diffusion from the tip, was evaluated during the manufacturing process. In addition, the simulations were consistent with the experimental results and showed apparent diffusion. Moreover, dextrans of different molecular weights labeled with FITC were chosen to fabricate the tip of microneedles for demonstrating their applicability. Finally, a micro-jetting system with a micro-nozzle (diameter: 80 μm) was developed to achieve the accurate and rapid loading of small amounts of FITC using the anti-diffusion and micro-jetting methods. Our method not only uses a simple and fast manufacturing process, but also fabricates the tips of microneedles more efficiently with FITC compared with the existing methods. We believe that the proposed method is essential for the clinical applications of the microneedle drug delivery platform.
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spelling pubmed-75995442020-11-01 Effective Dispensing Methods for Loading Drugs Only to the Tip of DNA Microneedles Bok, Moonjeong Zhao, Zhi-Jun Hwang, Soon Hyoung Kang, Hyeok-Joong Jeon, Sohee Ko, Jiwoo Jeong, Jiwon Song, Young Seok Lim, Eunju Jeong, Jun-Ho Pharmaceutics Article Here, we propose a novel and simple method to efficiently capture the diffusion of fluorescein isothiocyanate (FITC)-dextran from a biocompatible substance and load the drug only to the tip of DNA microneedles. A dispensing and suction method was chosen to fabricate the designed microneedles with efficient amounts of FITC as the drug model. Importantly, the vacuum process, which could influence the capturing of FITC diffusion from the tip, was evaluated during the manufacturing process. In addition, the simulations were consistent with the experimental results and showed apparent diffusion. Moreover, dextrans of different molecular weights labeled with FITC were chosen to fabricate the tip of microneedles for demonstrating their applicability. Finally, a micro-jetting system with a micro-nozzle (diameter: 80 μm) was developed to achieve the accurate and rapid loading of small amounts of FITC using the anti-diffusion and micro-jetting methods. Our method not only uses a simple and fast manufacturing process, but also fabricates the tips of microneedles more efficiently with FITC compared with the existing methods. We believe that the proposed method is essential for the clinical applications of the microneedle drug delivery platform. MDPI 2020-10-10 /pmc/articles/PMC7599544/ /pubmed/33050428 http://dx.doi.org/10.3390/pharmaceutics12100954 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bok, Moonjeong
Zhao, Zhi-Jun
Hwang, Soon Hyoung
Kang, Hyeok-Joong
Jeon, Sohee
Ko, Jiwoo
Jeong, Jiwon
Song, Young Seok
Lim, Eunju
Jeong, Jun-Ho
Effective Dispensing Methods for Loading Drugs Only to the Tip of DNA Microneedles
title Effective Dispensing Methods for Loading Drugs Only to the Tip of DNA Microneedles
title_full Effective Dispensing Methods for Loading Drugs Only to the Tip of DNA Microneedles
title_fullStr Effective Dispensing Methods for Loading Drugs Only to the Tip of DNA Microneedles
title_full_unstemmed Effective Dispensing Methods for Loading Drugs Only to the Tip of DNA Microneedles
title_short Effective Dispensing Methods for Loading Drugs Only to the Tip of DNA Microneedles
title_sort effective dispensing methods for loading drugs only to the tip of dna microneedles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599544/
https://www.ncbi.nlm.nih.gov/pubmed/33050428
http://dx.doi.org/10.3390/pharmaceutics12100954
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