Cargando…

Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool

The microbial diversity encompassed by the environmental biosphere is largely unexplored, although it represents an extensive source of new knowledge and potentially of novel enzymatic catalysts for biotechnological applications. To determine the taxonomy of microorganisms, proteotyping by tandem ma...

Descripción completa

Detalles Bibliográficos
Autores principales: Hayoun, Karim, Pible, Olivier, Petit, Pauline, Allain, François, Jouffret, Virginie, Culotta, Karen, Rivasseau, Corinne, Armengaud, Jean, Alpha-Bazin, Béatrice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599590/
https://www.ncbi.nlm.nih.gov/pubmed/33020444
http://dx.doi.org/10.3390/microorganisms8101525
_version_ 1783602913241726976
author Hayoun, Karim
Pible, Olivier
Petit, Pauline
Allain, François
Jouffret, Virginie
Culotta, Karen
Rivasseau, Corinne
Armengaud, Jean
Alpha-Bazin, Béatrice
author_facet Hayoun, Karim
Pible, Olivier
Petit, Pauline
Allain, François
Jouffret, Virginie
Culotta, Karen
Rivasseau, Corinne
Armengaud, Jean
Alpha-Bazin, Béatrice
author_sort Hayoun, Karim
collection PubMed
description The microbial diversity encompassed by the environmental biosphere is largely unexplored, although it represents an extensive source of new knowledge and potentially of novel enzymatic catalysts for biotechnological applications. To determine the taxonomy of microorganisms, proteotyping by tandem mass spectrometry has proved its efficiency. Its latest extension, phylopeptidomics, adds a biomass quantitation perspective for mixtures of microorganisms. Here, we present an application of phylopeptidomics to rapidly and sensitively screen microorganisms sampled from an industrial environment, i.e., a pool where radioactive material is stored. The power of this methodology is demonstrated through the identification of both prokaryotes and eukaryotes, whether as pure isolates or present as mixtures or consortia. In this study, we established accurate taxonomical identification of environmental prokaryotes belonging to the Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria phyla, as well as eukaryotes from the Ascomycota phylum. The results presented illustrate the potential of tandem mass spectrometry proteotyping, in particular phylopeptidomics, to screen for and rapidly identify microorganisms.
format Online
Article
Text
id pubmed-7599590
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-75995902020-11-01 Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool Hayoun, Karim Pible, Olivier Petit, Pauline Allain, François Jouffret, Virginie Culotta, Karen Rivasseau, Corinne Armengaud, Jean Alpha-Bazin, Béatrice Microorganisms Article The microbial diversity encompassed by the environmental biosphere is largely unexplored, although it represents an extensive source of new knowledge and potentially of novel enzymatic catalysts for biotechnological applications. To determine the taxonomy of microorganisms, proteotyping by tandem mass spectrometry has proved its efficiency. Its latest extension, phylopeptidomics, adds a biomass quantitation perspective for mixtures of microorganisms. Here, we present an application of phylopeptidomics to rapidly and sensitively screen microorganisms sampled from an industrial environment, i.e., a pool where radioactive material is stored. The power of this methodology is demonstrated through the identification of both prokaryotes and eukaryotes, whether as pure isolates or present as mixtures or consortia. In this study, we established accurate taxonomical identification of environmental prokaryotes belonging to the Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria phyla, as well as eukaryotes from the Ascomycota phylum. The results presented illustrate the potential of tandem mass spectrometry proteotyping, in particular phylopeptidomics, to screen for and rapidly identify microorganisms. MDPI 2020-10-04 /pmc/articles/PMC7599590/ /pubmed/33020444 http://dx.doi.org/10.3390/microorganisms8101525 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hayoun, Karim
Pible, Olivier
Petit, Pauline
Allain, François
Jouffret, Virginie
Culotta, Karen
Rivasseau, Corinne
Armengaud, Jean
Alpha-Bazin, Béatrice
Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool
title Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool
title_full Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool
title_fullStr Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool
title_full_unstemmed Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool
title_short Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool
title_sort proteotyping environmental microorganisms by phylopeptidomics: case study screening water from a radioactive material storage pool
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599590/
https://www.ncbi.nlm.nih.gov/pubmed/33020444
http://dx.doi.org/10.3390/microorganisms8101525
work_keys_str_mv AT hayounkarim proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool
AT pibleolivier proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool
AT petitpauline proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool
AT allainfrancois proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool
AT jouffretvirginie proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool
AT culottakaren proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool
AT rivasseaucorinne proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool
AT armengaudjean proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool
AT alphabazinbeatrice proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool