Cargando…
Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool
The microbial diversity encompassed by the environmental biosphere is largely unexplored, although it represents an extensive source of new knowledge and potentially of novel enzymatic catalysts for biotechnological applications. To determine the taxonomy of microorganisms, proteotyping by tandem ma...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599590/ https://www.ncbi.nlm.nih.gov/pubmed/33020444 http://dx.doi.org/10.3390/microorganisms8101525 |
_version_ | 1783602913241726976 |
---|---|
author | Hayoun, Karim Pible, Olivier Petit, Pauline Allain, François Jouffret, Virginie Culotta, Karen Rivasseau, Corinne Armengaud, Jean Alpha-Bazin, Béatrice |
author_facet | Hayoun, Karim Pible, Olivier Petit, Pauline Allain, François Jouffret, Virginie Culotta, Karen Rivasseau, Corinne Armengaud, Jean Alpha-Bazin, Béatrice |
author_sort | Hayoun, Karim |
collection | PubMed |
description | The microbial diversity encompassed by the environmental biosphere is largely unexplored, although it represents an extensive source of new knowledge and potentially of novel enzymatic catalysts for biotechnological applications. To determine the taxonomy of microorganisms, proteotyping by tandem mass spectrometry has proved its efficiency. Its latest extension, phylopeptidomics, adds a biomass quantitation perspective for mixtures of microorganisms. Here, we present an application of phylopeptidomics to rapidly and sensitively screen microorganisms sampled from an industrial environment, i.e., a pool where radioactive material is stored. The power of this methodology is demonstrated through the identification of both prokaryotes and eukaryotes, whether as pure isolates or present as mixtures or consortia. In this study, we established accurate taxonomical identification of environmental prokaryotes belonging to the Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria phyla, as well as eukaryotes from the Ascomycota phylum. The results presented illustrate the potential of tandem mass spectrometry proteotyping, in particular phylopeptidomics, to screen for and rapidly identify microorganisms. |
format | Online Article Text |
id | pubmed-7599590 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75995902020-11-01 Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool Hayoun, Karim Pible, Olivier Petit, Pauline Allain, François Jouffret, Virginie Culotta, Karen Rivasseau, Corinne Armengaud, Jean Alpha-Bazin, Béatrice Microorganisms Article The microbial diversity encompassed by the environmental biosphere is largely unexplored, although it represents an extensive source of new knowledge and potentially of novel enzymatic catalysts for biotechnological applications. To determine the taxonomy of microorganisms, proteotyping by tandem mass spectrometry has proved its efficiency. Its latest extension, phylopeptidomics, adds a biomass quantitation perspective for mixtures of microorganisms. Here, we present an application of phylopeptidomics to rapidly and sensitively screen microorganisms sampled from an industrial environment, i.e., a pool where radioactive material is stored. The power of this methodology is demonstrated through the identification of both prokaryotes and eukaryotes, whether as pure isolates or present as mixtures or consortia. In this study, we established accurate taxonomical identification of environmental prokaryotes belonging to the Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria phyla, as well as eukaryotes from the Ascomycota phylum. The results presented illustrate the potential of tandem mass spectrometry proteotyping, in particular phylopeptidomics, to screen for and rapidly identify microorganisms. MDPI 2020-10-04 /pmc/articles/PMC7599590/ /pubmed/33020444 http://dx.doi.org/10.3390/microorganisms8101525 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hayoun, Karim Pible, Olivier Petit, Pauline Allain, François Jouffret, Virginie Culotta, Karen Rivasseau, Corinne Armengaud, Jean Alpha-Bazin, Béatrice Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool |
title | Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool |
title_full | Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool |
title_fullStr | Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool |
title_full_unstemmed | Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool |
title_short | Proteotyping Environmental Microorganisms by Phylopeptidomics: Case Study Screening Water from a Radioactive Material Storage Pool |
title_sort | proteotyping environmental microorganisms by phylopeptidomics: case study screening water from a radioactive material storage pool |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599590/ https://www.ncbi.nlm.nih.gov/pubmed/33020444 http://dx.doi.org/10.3390/microorganisms8101525 |
work_keys_str_mv | AT hayounkarim proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool AT pibleolivier proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool AT petitpauline proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool AT allainfrancois proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool AT jouffretvirginie proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool AT culottakaren proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool AT rivasseaucorinne proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool AT armengaudjean proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool AT alphabazinbeatrice proteotypingenvironmentalmicroorganismsbyphylopeptidomicscasestudyscreeningwaterfromaradioactivematerialstoragepool |