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Silver Nanoplates for Colorimetric Determination of Xanthine in Human Plasma and in Fish Meat via Etching/Aggregation/Fusion Steps

Silver nanoplates (AgP) were prepared and used in a colorimetric method for the evaluation of Xanthine (Xan) in blood plasma and fish meat. The detection mechanism for Xan was observed to occur via etching of AgP particles/aggregation/fusion steps, resulting in a color change from blue to grey. Firs...

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Autores principales: Hsu, Hung-Cheng, Liao, Pei-Wen, Lee, Hsiang-Tzu, Liu, Wei-Chen, Ho, Mei-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599804/
https://www.ncbi.nlm.nih.gov/pubmed/33050252
http://dx.doi.org/10.3390/s20205739
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author Hsu, Hung-Cheng
Liao, Pei-Wen
Lee, Hsiang-Tzu
Liu, Wei-Chen
Ho, Mei-Lin
author_facet Hsu, Hung-Cheng
Liao, Pei-Wen
Lee, Hsiang-Tzu
Liu, Wei-Chen
Ho, Mei-Lin
author_sort Hsu, Hung-Cheng
collection PubMed
description Silver nanoplates (AgP) were prepared and used in a colorimetric method for the evaluation of Xanthine (Xan) in blood plasma and fish meat. The detection mechanism for Xan was observed to occur via etching of AgP particles/aggregation/fusion steps, resulting in a color change from blue to grey. First, the basic Xan solution is adsorbed through partial substitution of capping molecules around the AgP with Xan, and then intermolecular hydrogen bonds form between AgP and AgP. Subsequently, the titrant Xan solution further etches the AgP and finally fuses particles together. Owing to the step by step mechanism, the response range towards Xan has two linear regression ranges: 0.15–0.60 μM and 0.61–3.00 μM, respectively. The detection limit in the range of 0.15–0.60 μM is 0.011 μM (S/N = 3). AgP exhibits good selectivity for Xan over other potential interferents such as amino acids and blood proteins. AgP achieves rapid detection of Xan and can be applied to the satisfactory determination of Xan in blood plasma and fish meat. This colorimetric sensor is easy to use, cost effective, fast, selective and user friendly.
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spelling pubmed-75998042020-11-01 Silver Nanoplates for Colorimetric Determination of Xanthine in Human Plasma and in Fish Meat via Etching/Aggregation/Fusion Steps Hsu, Hung-Cheng Liao, Pei-Wen Lee, Hsiang-Tzu Liu, Wei-Chen Ho, Mei-Lin Sensors (Basel) Article Silver nanoplates (AgP) were prepared and used in a colorimetric method for the evaluation of Xanthine (Xan) in blood plasma and fish meat. The detection mechanism for Xan was observed to occur via etching of AgP particles/aggregation/fusion steps, resulting in a color change from blue to grey. First, the basic Xan solution is adsorbed through partial substitution of capping molecules around the AgP with Xan, and then intermolecular hydrogen bonds form between AgP and AgP. Subsequently, the titrant Xan solution further etches the AgP and finally fuses particles together. Owing to the step by step mechanism, the response range towards Xan has two linear regression ranges: 0.15–0.60 μM and 0.61–3.00 μM, respectively. The detection limit in the range of 0.15–0.60 μM is 0.011 μM (S/N = 3). AgP exhibits good selectivity for Xan over other potential interferents such as amino acids and blood proteins. AgP achieves rapid detection of Xan and can be applied to the satisfactory determination of Xan in blood plasma and fish meat. This colorimetric sensor is easy to use, cost effective, fast, selective and user friendly. MDPI 2020-10-09 /pmc/articles/PMC7599804/ /pubmed/33050252 http://dx.doi.org/10.3390/s20205739 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hsu, Hung-Cheng
Liao, Pei-Wen
Lee, Hsiang-Tzu
Liu, Wei-Chen
Ho, Mei-Lin
Silver Nanoplates for Colorimetric Determination of Xanthine in Human Plasma and in Fish Meat via Etching/Aggregation/Fusion Steps
title Silver Nanoplates for Colorimetric Determination of Xanthine in Human Plasma and in Fish Meat via Etching/Aggregation/Fusion Steps
title_full Silver Nanoplates for Colorimetric Determination of Xanthine in Human Plasma and in Fish Meat via Etching/Aggregation/Fusion Steps
title_fullStr Silver Nanoplates for Colorimetric Determination of Xanthine in Human Plasma and in Fish Meat via Etching/Aggregation/Fusion Steps
title_full_unstemmed Silver Nanoplates for Colorimetric Determination of Xanthine in Human Plasma and in Fish Meat via Etching/Aggregation/Fusion Steps
title_short Silver Nanoplates for Colorimetric Determination of Xanthine in Human Plasma and in Fish Meat via Etching/Aggregation/Fusion Steps
title_sort silver nanoplates for colorimetric determination of xanthine in human plasma and in fish meat via etching/aggregation/fusion steps
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599804/
https://www.ncbi.nlm.nih.gov/pubmed/33050252
http://dx.doi.org/10.3390/s20205739
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