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Effect of the Albumin Corona on the Toxicity of Combined Graphene Oxide and Cadmium to Daphnia magna and Integration of the Datasets into the NanoCommons Knowledge Base
In this work, we evaluated the effect of protein corona formation on graphene oxide (GO) mixture toxicity testing (i.e., co-exposure) using the Daphnia magna model and assessing acute toxicity determined as immobilisation. Cadmium (Cd(2+)) and bovine serum albumin (BSA) were selected as co-pollutant...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599915/ https://www.ncbi.nlm.nih.gov/pubmed/33003330 http://dx.doi.org/10.3390/nano10101936 |
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author | Martinez, Diego Stéfani T. Da Silva, Gabriela H. de Medeiros, Aline Maria Z. Khan, Latif U. Papadiamantis, Anastasios G. Lynch, Iseult |
author_facet | Martinez, Diego Stéfani T. Da Silva, Gabriela H. de Medeiros, Aline Maria Z. Khan, Latif U. Papadiamantis, Anastasios G. Lynch, Iseult |
author_sort | Martinez, Diego Stéfani T. |
collection | PubMed |
description | In this work, we evaluated the effect of protein corona formation on graphene oxide (GO) mixture toxicity testing (i.e., co-exposure) using the Daphnia magna model and assessing acute toxicity determined as immobilisation. Cadmium (Cd(2+)) and bovine serum albumin (BSA) were selected as co-pollutant and protein model system, respectively. Albumin corona formation on GO dramatically increased its colloidal stability (ca. 60%) and Cd(2+) adsorption capacity (ca. 4.5 times) in reconstituted water (Daphnia medium). The acute toxicity values (48 h-EC(50)) observed were 0.18 mg L(−1) for Cd(2+)-only and 0.29 and 0.61 mg L(−1) following co-exposure of Cd(2+) with GO and BSA@GO materials, respectively, at a fixed non-toxic concentration of 1.0 mg L(−1). After coronation of GO with BSA, a reduction in cadmium toxicity of 110 % and 238% was achieved when compared to bare GO and Cd(2+)-only, respectively. Integration of datasets associated with graphene-based materials, heavy metals and mixture toxicity is essential to enable re-use of the data and facilitate nanoinformatics approaches for design of safer nanomaterials for water quality monitoring and remediation technologies. Hence, all data from this work were annotated and integrated into the NanoCommons Knowledge Base, connecting the experimental data to nanoinformatics platforms under the FAIR data principles and making them interoperable with similar datasets. |
format | Online Article Text |
id | pubmed-7599915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-75999152020-11-01 Effect of the Albumin Corona on the Toxicity of Combined Graphene Oxide and Cadmium to Daphnia magna and Integration of the Datasets into the NanoCommons Knowledge Base Martinez, Diego Stéfani T. Da Silva, Gabriela H. de Medeiros, Aline Maria Z. Khan, Latif U. Papadiamantis, Anastasios G. Lynch, Iseult Nanomaterials (Basel) Article In this work, we evaluated the effect of protein corona formation on graphene oxide (GO) mixture toxicity testing (i.e., co-exposure) using the Daphnia magna model and assessing acute toxicity determined as immobilisation. Cadmium (Cd(2+)) and bovine serum albumin (BSA) were selected as co-pollutant and protein model system, respectively. Albumin corona formation on GO dramatically increased its colloidal stability (ca. 60%) and Cd(2+) adsorption capacity (ca. 4.5 times) in reconstituted water (Daphnia medium). The acute toxicity values (48 h-EC(50)) observed were 0.18 mg L(−1) for Cd(2+)-only and 0.29 and 0.61 mg L(−1) following co-exposure of Cd(2+) with GO and BSA@GO materials, respectively, at a fixed non-toxic concentration of 1.0 mg L(−1). After coronation of GO with BSA, a reduction in cadmium toxicity of 110 % and 238% was achieved when compared to bare GO and Cd(2+)-only, respectively. Integration of datasets associated with graphene-based materials, heavy metals and mixture toxicity is essential to enable re-use of the data and facilitate nanoinformatics approaches for design of safer nanomaterials for water quality monitoring and remediation technologies. Hence, all data from this work were annotated and integrated into the NanoCommons Knowledge Base, connecting the experimental data to nanoinformatics platforms under the FAIR data principles and making them interoperable with similar datasets. MDPI 2020-09-29 /pmc/articles/PMC7599915/ /pubmed/33003330 http://dx.doi.org/10.3390/nano10101936 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Martinez, Diego Stéfani T. Da Silva, Gabriela H. de Medeiros, Aline Maria Z. Khan, Latif U. Papadiamantis, Anastasios G. Lynch, Iseult Effect of the Albumin Corona on the Toxicity of Combined Graphene Oxide and Cadmium to Daphnia magna and Integration of the Datasets into the NanoCommons Knowledge Base |
title | Effect of the Albumin Corona on the Toxicity of Combined Graphene Oxide and Cadmium to Daphnia magna and Integration of the Datasets into the NanoCommons Knowledge Base |
title_full | Effect of the Albumin Corona on the Toxicity of Combined Graphene Oxide and Cadmium to Daphnia magna and Integration of the Datasets into the NanoCommons Knowledge Base |
title_fullStr | Effect of the Albumin Corona on the Toxicity of Combined Graphene Oxide and Cadmium to Daphnia magna and Integration of the Datasets into the NanoCommons Knowledge Base |
title_full_unstemmed | Effect of the Albumin Corona on the Toxicity of Combined Graphene Oxide and Cadmium to Daphnia magna and Integration of the Datasets into the NanoCommons Knowledge Base |
title_short | Effect of the Albumin Corona on the Toxicity of Combined Graphene Oxide and Cadmium to Daphnia magna and Integration of the Datasets into the NanoCommons Knowledge Base |
title_sort | effect of the albumin corona on the toxicity of combined graphene oxide and cadmium to daphnia magna and integration of the datasets into the nanocommons knowledge base |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599915/ https://www.ncbi.nlm.nih.gov/pubmed/33003330 http://dx.doi.org/10.3390/nano10101936 |
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