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Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds

In this study, magnetic maghemite nanoparticles, which belong to the group of metal oxides, were functionalized with chitosan, a non-toxic, hydrophilic, biocompatible, biodegradable biopolymer with anti-bacterial effects. This was done using different synthesis methods, and a comparison of the prope...

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Autores principales: Hojnik Podrepšek, Gordana, Knez, Željko, Leitgeb, Maja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599998/
https://www.ncbi.nlm.nih.gov/pubmed/32992815
http://dx.doi.org/10.3390/nano10101913
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author Hojnik Podrepšek, Gordana
Knez, Željko
Leitgeb, Maja
author_facet Hojnik Podrepšek, Gordana
Knez, Željko
Leitgeb, Maja
author_sort Hojnik Podrepšek, Gordana
collection PubMed
description In this study, magnetic maghemite nanoparticles, which belong to the group of metal oxides, were functionalized with chitosan, a non-toxic, hydrophilic, biocompatible, biodegradable biopolymer with anti-bacterial effects. This was done using different synthesis methods, and a comparison of the properties of the synthesized chitosan functionalized maghemite nanoparticles was conducted. Characterization was performed using scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM). Characterizations of size distribution were performed using dynamic light scattering (DLS) measurements and laser granulometry. A chitosan functionalization layer was confirmed using potentiometric titration on variously synthesized chitosan functionalized maghemite nanoparticles, which is important for further immobilization of bioactive compounds. Furthermore, after activation of chitosan functionalized maghemite nanoparticles with glutaraldehyde (GA) or pentaethylenehexamine (PEHA), immobilization studies of enzyme cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) were conducted. Factors influencing the immobilization of enzymes, such as type and concentration of activating reagent, mass ratio between carrier and enzyme, immobilization time and enzyme concentration, were investigated. Briefly, microparticles made using the chitosan suspension cross-linking process (MC2) proved to be the most suitable for obtaining the highest activity of immobilized enzyme, and nanoparticles functionalized with chitosan using the covalent binding method (MC3) could compete with MC2 for their applications.
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spelling pubmed-75999982020-11-01 Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds Hojnik Podrepšek, Gordana Knez, Željko Leitgeb, Maja Nanomaterials (Basel) Article In this study, magnetic maghemite nanoparticles, which belong to the group of metal oxides, were functionalized with chitosan, a non-toxic, hydrophilic, biocompatible, biodegradable biopolymer with anti-bacterial effects. This was done using different synthesis methods, and a comparison of the properties of the synthesized chitosan functionalized maghemite nanoparticles was conducted. Characterization was performed using scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM). Characterizations of size distribution were performed using dynamic light scattering (DLS) measurements and laser granulometry. A chitosan functionalization layer was confirmed using potentiometric titration on variously synthesized chitosan functionalized maghemite nanoparticles, which is important for further immobilization of bioactive compounds. Furthermore, after activation of chitosan functionalized maghemite nanoparticles with glutaraldehyde (GA) or pentaethylenehexamine (PEHA), immobilization studies of enzyme cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) were conducted. Factors influencing the immobilization of enzymes, such as type and concentration of activating reagent, mass ratio between carrier and enzyme, immobilization time and enzyme concentration, were investigated. Briefly, microparticles made using the chitosan suspension cross-linking process (MC2) proved to be the most suitable for obtaining the highest activity of immobilized enzyme, and nanoparticles functionalized with chitosan using the covalent binding method (MC3) could compete with MC2 for their applications. MDPI 2020-09-25 /pmc/articles/PMC7599998/ /pubmed/32992815 http://dx.doi.org/10.3390/nano10101913 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hojnik Podrepšek, Gordana
Knez, Željko
Leitgeb, Maja
Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds
title Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds
title_full Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds
title_fullStr Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds
title_full_unstemmed Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds
title_short Development of Chitosan Functionalized Magnetic Nanoparticles with Bioactive Compounds
title_sort development of chitosan functionalized magnetic nanoparticles with bioactive compounds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7599998/
https://www.ncbi.nlm.nih.gov/pubmed/32992815
http://dx.doi.org/10.3390/nano10101913
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