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Biopsy-Controlled Non-Invasive Quantification of Collagen Type VI in Kidney Transplant Recipients: A Post-Hoc Analysis of the MECANO Trial
The PRO-C6 assay, a reflection of collagen type VI synthesis, has been proposed as a non-invasive early biomarker of kidney fibrosis. We aimed to investigate cross-sectional and longitudinal associations between plasma and urine PRO-C6 and proven histological changes after kidney transplantation. Th...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600059/ https://www.ncbi.nlm.nih.gov/pubmed/33036366 http://dx.doi.org/10.3390/jcm9103216 |
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author | Yepes-Calderón, Manuela Sotomayor, Camilo G. Rasmussen, Daniel Guldager Kring Hijmans, Ryanne S. te Velde-Keyzer, Charlotte A. van Londen, Marco van Dijk, Marja Diepstra, Arjan Berger, Stefan P. Karsdal, Morten Asser Bemelman, Frederike J. de Fijter, Johan W. Kers, Jesper Florquin, Sandrine Genovese, Federica Bakker, Stephan J. L. Sanders, Jan-Stephan Van Den Born, Jacob |
author_facet | Yepes-Calderón, Manuela Sotomayor, Camilo G. Rasmussen, Daniel Guldager Kring Hijmans, Ryanne S. te Velde-Keyzer, Charlotte A. van Londen, Marco van Dijk, Marja Diepstra, Arjan Berger, Stefan P. Karsdal, Morten Asser Bemelman, Frederike J. de Fijter, Johan W. Kers, Jesper Florquin, Sandrine Genovese, Federica Bakker, Stephan J. L. Sanders, Jan-Stephan Van Den Born, Jacob |
author_sort | Yepes-Calderón, Manuela |
collection | PubMed |
description | The PRO-C6 assay, a reflection of collagen type VI synthesis, has been proposed as a non-invasive early biomarker of kidney fibrosis. We aimed to investigate cross-sectional and longitudinal associations between plasma and urine PRO-C6 and proven histological changes after kidney transplantation. The current study is a post-hoc analysis of 94 participants of the MECANO trial, a 24-month prospective, multicenter, open-label, randomized, controlled trial aimed at comparing everolimus-based vs. cyclosporine-based immunosuppression. PRO-C6 was measured in plasma and urine samples collected 6 and 24 months post-transplantation. Fibrosis was evaluated in biopsies collected at the same time points by Banff interstitial fibrosis/tubular atrophy (IF/TA) scoring and collagen staining (Picro Sirius Red; PSR); inflammation was evaluated by the tubulo-interstitial inflammation score (ti-score). Linear regression analyses were performed. Six-month plasma PRO-C6 was cross-sectionally associated with IF/TA score (Std. β = 0.34), and prospectively with 24-month IF/TA score and ti-score (Std. β = 0.24 and 0.23, respectively) (p < 0.05 for all). No significant associations were found between urine PRO-C6 and any of the biopsy findings. Fibrotic changes and urine PRO-C6 behaved differentially over time according to immunosuppressive therapy. These results are a first step towards non-invasive fibrosis detection after kidney transplantation by means of collagen VI synthesis measurement, and further research is required. |
format | Online Article Text |
id | pubmed-7600059 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76000592020-11-01 Biopsy-Controlled Non-Invasive Quantification of Collagen Type VI in Kidney Transplant Recipients: A Post-Hoc Analysis of the MECANO Trial Yepes-Calderón, Manuela Sotomayor, Camilo G. Rasmussen, Daniel Guldager Kring Hijmans, Ryanne S. te Velde-Keyzer, Charlotte A. van Londen, Marco van Dijk, Marja Diepstra, Arjan Berger, Stefan P. Karsdal, Morten Asser Bemelman, Frederike J. de Fijter, Johan W. Kers, Jesper Florquin, Sandrine Genovese, Federica Bakker, Stephan J. L. Sanders, Jan-Stephan Van Den Born, Jacob J Clin Med Article The PRO-C6 assay, a reflection of collagen type VI synthesis, has been proposed as a non-invasive early biomarker of kidney fibrosis. We aimed to investigate cross-sectional and longitudinal associations between plasma and urine PRO-C6 and proven histological changes after kidney transplantation. The current study is a post-hoc analysis of 94 participants of the MECANO trial, a 24-month prospective, multicenter, open-label, randomized, controlled trial aimed at comparing everolimus-based vs. cyclosporine-based immunosuppression. PRO-C6 was measured in plasma and urine samples collected 6 and 24 months post-transplantation. Fibrosis was evaluated in biopsies collected at the same time points by Banff interstitial fibrosis/tubular atrophy (IF/TA) scoring and collagen staining (Picro Sirius Red; PSR); inflammation was evaluated by the tubulo-interstitial inflammation score (ti-score). Linear regression analyses were performed. Six-month plasma PRO-C6 was cross-sectionally associated with IF/TA score (Std. β = 0.34), and prospectively with 24-month IF/TA score and ti-score (Std. β = 0.24 and 0.23, respectively) (p < 0.05 for all). No significant associations were found between urine PRO-C6 and any of the biopsy findings. Fibrotic changes and urine PRO-C6 behaved differentially over time according to immunosuppressive therapy. These results are a first step towards non-invasive fibrosis detection after kidney transplantation by means of collagen VI synthesis measurement, and further research is required. MDPI 2020-10-07 /pmc/articles/PMC7600059/ /pubmed/33036366 http://dx.doi.org/10.3390/jcm9103216 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yepes-Calderón, Manuela Sotomayor, Camilo G. Rasmussen, Daniel Guldager Kring Hijmans, Ryanne S. te Velde-Keyzer, Charlotte A. van Londen, Marco van Dijk, Marja Diepstra, Arjan Berger, Stefan P. Karsdal, Morten Asser Bemelman, Frederike J. de Fijter, Johan W. Kers, Jesper Florquin, Sandrine Genovese, Federica Bakker, Stephan J. L. Sanders, Jan-Stephan Van Den Born, Jacob Biopsy-Controlled Non-Invasive Quantification of Collagen Type VI in Kidney Transplant Recipients: A Post-Hoc Analysis of the MECANO Trial |
title | Biopsy-Controlled Non-Invasive Quantification of Collagen Type VI in Kidney Transplant Recipients: A Post-Hoc Analysis of the MECANO Trial |
title_full | Biopsy-Controlled Non-Invasive Quantification of Collagen Type VI in Kidney Transplant Recipients: A Post-Hoc Analysis of the MECANO Trial |
title_fullStr | Biopsy-Controlled Non-Invasive Quantification of Collagen Type VI in Kidney Transplant Recipients: A Post-Hoc Analysis of the MECANO Trial |
title_full_unstemmed | Biopsy-Controlled Non-Invasive Quantification of Collagen Type VI in Kidney Transplant Recipients: A Post-Hoc Analysis of the MECANO Trial |
title_short | Biopsy-Controlled Non-Invasive Quantification of Collagen Type VI in Kidney Transplant Recipients: A Post-Hoc Analysis of the MECANO Trial |
title_sort | biopsy-controlled non-invasive quantification of collagen type vi in kidney transplant recipients: a post-hoc analysis of the mecano trial |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600059/ https://www.ncbi.nlm.nih.gov/pubmed/33036366 http://dx.doi.org/10.3390/jcm9103216 |
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