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Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants

Capripox viruses, with their members “lumpy skin disease virus (LSDV)”, “goatpox virus (GTPV)” and “sheeppox virus (SPPV)”, are described as the most serious pox diseases of production animals. A GTPV isolate and a SPPV isolate were sequenced in a combined approach using nanopore MinION sequencing t...

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Autores principales: Wolff, Janika, King, Jacqueline, Moritz, Tom, Pohlmann, Anne, Hoffmann, Donata, Beer, Martin, Hoffmann, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600078/
https://www.ncbi.nlm.nih.gov/pubmed/32998423
http://dx.doi.org/10.3390/v12101098
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author Wolff, Janika
King, Jacqueline
Moritz, Tom
Pohlmann, Anne
Hoffmann, Donata
Beer, Martin
Hoffmann, Bernd
author_facet Wolff, Janika
King, Jacqueline
Moritz, Tom
Pohlmann, Anne
Hoffmann, Donata
Beer, Martin
Hoffmann, Bernd
author_sort Wolff, Janika
collection PubMed
description Capripox viruses, with their members “lumpy skin disease virus (LSDV)”, “goatpox virus (GTPV)” and “sheeppox virus (SPPV)”, are described as the most serious pox diseases of production animals. A GTPV isolate and a SPPV isolate were sequenced in a combined approach using nanopore MinION sequencing to obtain long reads and Illumina high throughput sequencing for short precise reads to gain full-length high-quality genome sequences. Concomitantly, sheep and goats were inoculated with SPPV and GTPV strains, respectively. During the animal trial, varying infection routes were compared: a combined intravenous and subcutaneous infection, an only intranasal infection, and the contact infection between naïve and inoculated animals. Sheep inoculated with SPPV showed no clinical signs, only a very small number of genome-positive samples and a low-level antibody reaction. In contrast, all GTPV inoculated or in-contact goats developed severe clinical signs with high viral genome loads observed in all tested matrices. Furthermore, seroconversion was detected in nearly all goats and no differences concerning the severity of the disease depending on the inoculation route were observed. Conclusively, the employed SPPV strain has the properties of an attenuated vaccine strain, consistent with the genetic data, whereas the GTPV strain represents a highly virulent field strain.
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spelling pubmed-76000782020-11-01 Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants Wolff, Janika King, Jacqueline Moritz, Tom Pohlmann, Anne Hoffmann, Donata Beer, Martin Hoffmann, Bernd Viruses Article Capripox viruses, with their members “lumpy skin disease virus (LSDV)”, “goatpox virus (GTPV)” and “sheeppox virus (SPPV)”, are described as the most serious pox diseases of production animals. A GTPV isolate and a SPPV isolate were sequenced in a combined approach using nanopore MinION sequencing to obtain long reads and Illumina high throughput sequencing for short precise reads to gain full-length high-quality genome sequences. Concomitantly, sheep and goats were inoculated with SPPV and GTPV strains, respectively. During the animal trial, varying infection routes were compared: a combined intravenous and subcutaneous infection, an only intranasal infection, and the contact infection between naïve and inoculated animals. Sheep inoculated with SPPV showed no clinical signs, only a very small number of genome-positive samples and a low-level antibody reaction. In contrast, all GTPV inoculated or in-contact goats developed severe clinical signs with high viral genome loads observed in all tested matrices. Furthermore, seroconversion was detected in nearly all goats and no differences concerning the severity of the disease depending on the inoculation route were observed. Conclusively, the employed SPPV strain has the properties of an attenuated vaccine strain, consistent with the genetic data, whereas the GTPV strain represents a highly virulent field strain. MDPI 2020-09-28 /pmc/articles/PMC7600078/ /pubmed/32998423 http://dx.doi.org/10.3390/v12101098 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wolff, Janika
King, Jacqueline
Moritz, Tom
Pohlmann, Anne
Hoffmann, Donata
Beer, Martin
Hoffmann, Bernd
Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants
title Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants
title_full Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants
title_fullStr Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants
title_full_unstemmed Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants
title_short Experimental Infection and Genetic Characterization of Two Different Capripox Virus Isolates in Small Ruminants
title_sort experimental infection and genetic characterization of two different capripox virus isolates in small ruminants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600078/
https://www.ncbi.nlm.nih.gov/pubmed/32998423
http://dx.doi.org/10.3390/v12101098
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