Cargando…

Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents

Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are novel endogenous lipids with important physiological functions in mammals. We previously identified a new type of FAHFAs, named short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs), with acetyl or propyl esters of hydroxy fatt...

Descripción completa

Detalles Bibliográficos
Autores principales: B. Gowda, Siddabasave Gowda, Gowda, Divyavani, Liang, Chongsheng, Li, Yonghan, Kawakami, Kentaro, Fukiya, Satoru, Yokota, Atsushi, Chiba, Hitoshi, Hui, Shu-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600112/
https://www.ncbi.nlm.nih.gov/pubmed/33050007
http://dx.doi.org/10.3390/metabo10100398
_version_ 1783603055897346048
author B. Gowda, Siddabasave Gowda
Gowda, Divyavani
Liang, Chongsheng
Li, Yonghan
Kawakami, Kentaro
Fukiya, Satoru
Yokota, Atsushi
Chiba, Hitoshi
Hui, Shu-Ping
author_facet B. Gowda, Siddabasave Gowda
Gowda, Divyavani
Liang, Chongsheng
Li, Yonghan
Kawakami, Kentaro
Fukiya, Satoru
Yokota, Atsushi
Chiba, Hitoshi
Hui, Shu-Ping
author_sort B. Gowda, Siddabasave Gowda
collection PubMed
description Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are novel endogenous lipids with important physiological functions in mammals. We previously identified a new type of FAHFAs, named short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs), with acetyl or propyl esters of hydroxy fatty acids of carbon chains, C ≥ 20. However, sensitive determination of SFAHFAs is still a challenge, due to their high structural similarity and low abundance in biological samples. This study employs one-step chemical derivatization following total lipid extraction using 2-dimethylaminoethylamine (DMED) for enhanced detection of SFAHFAs. The labeled extracts were subjected to ultrahigh performance liquid chromatography coupled to linear ion trap quadrupole-Orbitrap mass spectrometry (UHPLC/LTQ-Orbitrap MS). Our results demonstrated that the detection sensitivities of SFAHFAs increased after DMED labeling, and is highly helpful in discovering six additional novel SFAHFAs in the cecum and colon contents of WKAH/HKmSlc rats fed with normal and high-fat diet (HFD). The identified DMED labeled SFAHFAs were characterized by their detailed MS/MS analysis, and their plausible fragmentation patterns were proposed. The concentrations of SFAHFAs were significantly reduced in the cecum of HFD group compared to the control. Hence, the proposed method could be a promising tool to apply for the enhanced detection of SFAHFAs in various biological matrices, which in turn facilitate the understanding of their sources, and physiological functions of these novel lipids.
format Online
Article
Text
id pubmed-7600112
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76001122020-11-01 Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents B. Gowda, Siddabasave Gowda Gowda, Divyavani Liang, Chongsheng Li, Yonghan Kawakami, Kentaro Fukiya, Satoru Yokota, Atsushi Chiba, Hitoshi Hui, Shu-Ping Metabolites Article Branched fatty acid esters of hydroxy fatty acids (FAHFAs) are novel endogenous lipids with important physiological functions in mammals. We previously identified a new type of FAHFAs, named short-chain fatty acid esterified hydroxy fatty acids (SFAHFAs), with acetyl or propyl esters of hydroxy fatty acids of carbon chains, C ≥ 20. However, sensitive determination of SFAHFAs is still a challenge, due to their high structural similarity and low abundance in biological samples. This study employs one-step chemical derivatization following total lipid extraction using 2-dimethylaminoethylamine (DMED) for enhanced detection of SFAHFAs. The labeled extracts were subjected to ultrahigh performance liquid chromatography coupled to linear ion trap quadrupole-Orbitrap mass spectrometry (UHPLC/LTQ-Orbitrap MS). Our results demonstrated that the detection sensitivities of SFAHFAs increased after DMED labeling, and is highly helpful in discovering six additional novel SFAHFAs in the cecum and colon contents of WKAH/HKmSlc rats fed with normal and high-fat diet (HFD). The identified DMED labeled SFAHFAs were characterized by their detailed MS/MS analysis, and their plausible fragmentation patterns were proposed. The concentrations of SFAHFAs were significantly reduced in the cecum of HFD group compared to the control. Hence, the proposed method could be a promising tool to apply for the enhanced detection of SFAHFAs in various biological matrices, which in turn facilitate the understanding of their sources, and physiological functions of these novel lipids. MDPI 2020-10-08 /pmc/articles/PMC7600112/ /pubmed/33050007 http://dx.doi.org/10.3390/metabo10100398 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
B. Gowda, Siddabasave Gowda
Gowda, Divyavani
Liang, Chongsheng
Li, Yonghan
Kawakami, Kentaro
Fukiya, Satoru
Yokota, Atsushi
Chiba, Hitoshi
Hui, Shu-Ping
Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents
title Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents
title_full Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents
title_fullStr Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents
title_full_unstemmed Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents
title_short Chemical Labeling Assisted Detection and Identification of Short Chain Fatty Acid Esters of Hydroxy Fatty Acid in Rat Colon and Cecum Contents
title_sort chemical labeling assisted detection and identification of short chain fatty acid esters of hydroxy fatty acid in rat colon and cecum contents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600112/
https://www.ncbi.nlm.nih.gov/pubmed/33050007
http://dx.doi.org/10.3390/metabo10100398
work_keys_str_mv AT bgowdasiddabasavegowda chemicallabelingassisteddetectionandidentificationofshortchainfattyacidestersofhydroxyfattyacidinratcolonandcecumcontents
AT gowdadivyavani chemicallabelingassisteddetectionandidentificationofshortchainfattyacidestersofhydroxyfattyacidinratcolonandcecumcontents
AT liangchongsheng chemicallabelingassisteddetectionandidentificationofshortchainfattyacidestersofhydroxyfattyacidinratcolonandcecumcontents
AT liyonghan chemicallabelingassisteddetectionandidentificationofshortchainfattyacidestersofhydroxyfattyacidinratcolonandcecumcontents
AT kawakamikentaro chemicallabelingassisteddetectionandidentificationofshortchainfattyacidestersofhydroxyfattyacidinratcolonandcecumcontents
AT fukiyasatoru chemicallabelingassisteddetectionandidentificationofshortchainfattyacidestersofhydroxyfattyacidinratcolonandcecumcontents
AT yokotaatsushi chemicallabelingassisteddetectionandidentificationofshortchainfattyacidestersofhydroxyfattyacidinratcolonandcecumcontents
AT chibahitoshi chemicallabelingassisteddetectionandidentificationofshortchainfattyacidestersofhydroxyfattyacidinratcolonandcecumcontents
AT huishuping chemicallabelingassisteddetectionandidentificationofshortchainfattyacidestersofhydroxyfattyacidinratcolonandcecumcontents