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House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection
Adenovirus (AdV) infections in the respiratory tract may cause asthma exacerbation and allergic predisposition, and the house dust mite (HDM) may aggravate virus-induced asthma exacerbations. However, the underlying mechanisms of whether and how AdV affects asthmatic patients remains unclear. To add...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600414/ https://www.ncbi.nlm.nih.gov/pubmed/33050625 http://dx.doi.org/10.3390/v12101151 |
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author | Aydin, Malik Naumova, Ella A. Paulsen, Friedrich Zhang, Wenli Gopon, Felix Theis, Christian Lutz, Sören Ehrke-Schulz, Eric Arnold, Wolfgang H. Wirth, Stefan Ehrhardt, Anja |
author_facet | Aydin, Malik Naumova, Ella A. Paulsen, Friedrich Zhang, Wenli Gopon, Felix Theis, Christian Lutz, Sören Ehrke-Schulz, Eric Arnold, Wolfgang H. Wirth, Stefan Ehrhardt, Anja |
author_sort | Aydin, Malik |
collection | PubMed |
description | Adenovirus (AdV) infections in the respiratory tract may cause asthma exacerbation and allergic predisposition, and the house dust mite (HDM) may aggravate virus-induced asthma exacerbations. However, the underlying mechanisms of whether and how AdV affects asthmatic patients remains unclear. To address this question, we investigated nasal epithelial cells (NAEPCs) derived from a pediatric exacerbation study cohort for experimental analyses. We analyzed twenty-one different green-fluorescent protein- and luciferase-tagged AdV types in submerged 2D and organotypic 3D cell culture models. Transduction experiments revealed robust transduction of AdV type 5 (AdV5) in NAEPCs, which was associated with an increased uptake of AdV5 in the presence of HDM. In healthy and asthmatic NAEPCs exposed to HDM before infection, we observed a time- and dose-dependent increase of AdV5 uptake associated with upregulation of entry receptors for AdV5. Furthermore, electron microscopic and histologic analyses of 3D cell cultures revealed an impairment of the respiratory cilia after HDM exposition. This ex vivo pilot study shows the impact of AdV infection and HDM exposition in a primary cell culture model for asthma. |
format | Online Article Text |
id | pubmed-7600414 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76004142020-11-01 House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection Aydin, Malik Naumova, Ella A. Paulsen, Friedrich Zhang, Wenli Gopon, Felix Theis, Christian Lutz, Sören Ehrke-Schulz, Eric Arnold, Wolfgang H. Wirth, Stefan Ehrhardt, Anja Viruses Article Adenovirus (AdV) infections in the respiratory tract may cause asthma exacerbation and allergic predisposition, and the house dust mite (HDM) may aggravate virus-induced asthma exacerbations. However, the underlying mechanisms of whether and how AdV affects asthmatic patients remains unclear. To address this question, we investigated nasal epithelial cells (NAEPCs) derived from a pediatric exacerbation study cohort for experimental analyses. We analyzed twenty-one different green-fluorescent protein- and luciferase-tagged AdV types in submerged 2D and organotypic 3D cell culture models. Transduction experiments revealed robust transduction of AdV type 5 (AdV5) in NAEPCs, which was associated with an increased uptake of AdV5 in the presence of HDM. In healthy and asthmatic NAEPCs exposed to HDM before infection, we observed a time- and dose-dependent increase of AdV5 uptake associated with upregulation of entry receptors for AdV5. Furthermore, electron microscopic and histologic analyses of 3D cell cultures revealed an impairment of the respiratory cilia after HDM exposition. This ex vivo pilot study shows the impact of AdV infection and HDM exposition in a primary cell culture model for asthma. MDPI 2020-10-11 /pmc/articles/PMC7600414/ /pubmed/33050625 http://dx.doi.org/10.3390/v12101151 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Aydin, Malik Naumova, Ella A. Paulsen, Friedrich Zhang, Wenli Gopon, Felix Theis, Christian Lutz, Sören Ehrke-Schulz, Eric Arnold, Wolfgang H. Wirth, Stefan Ehrhardt, Anja House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection |
title | House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection |
title_full | House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection |
title_fullStr | House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection |
title_full_unstemmed | House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection |
title_short | House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection |
title_sort | house dust mite exposure causes increased susceptibility of nasal epithelial cells to adenovirus infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600414/ https://www.ncbi.nlm.nih.gov/pubmed/33050625 http://dx.doi.org/10.3390/v12101151 |
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