Cargando…

House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection

Adenovirus (AdV) infections in the respiratory tract may cause asthma exacerbation and allergic predisposition, and the house dust mite (HDM) may aggravate virus-induced asthma exacerbations. However, the underlying mechanisms of whether and how AdV affects asthmatic patients remains unclear. To add...

Descripción completa

Detalles Bibliográficos
Autores principales: Aydin, Malik, Naumova, Ella A., Paulsen, Friedrich, Zhang, Wenli, Gopon, Felix, Theis, Christian, Lutz, Sören, Ehrke-Schulz, Eric, Arnold, Wolfgang H., Wirth, Stefan, Ehrhardt, Anja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600414/
https://www.ncbi.nlm.nih.gov/pubmed/33050625
http://dx.doi.org/10.3390/v12101151
_version_ 1783603138253553664
author Aydin, Malik
Naumova, Ella A.
Paulsen, Friedrich
Zhang, Wenli
Gopon, Felix
Theis, Christian
Lutz, Sören
Ehrke-Schulz, Eric
Arnold, Wolfgang H.
Wirth, Stefan
Ehrhardt, Anja
author_facet Aydin, Malik
Naumova, Ella A.
Paulsen, Friedrich
Zhang, Wenli
Gopon, Felix
Theis, Christian
Lutz, Sören
Ehrke-Schulz, Eric
Arnold, Wolfgang H.
Wirth, Stefan
Ehrhardt, Anja
author_sort Aydin, Malik
collection PubMed
description Adenovirus (AdV) infections in the respiratory tract may cause asthma exacerbation and allergic predisposition, and the house dust mite (HDM) may aggravate virus-induced asthma exacerbations. However, the underlying mechanisms of whether and how AdV affects asthmatic patients remains unclear. To address this question, we investigated nasal epithelial cells (NAEPCs) derived from a pediatric exacerbation study cohort for experimental analyses. We analyzed twenty-one different green-fluorescent protein- and luciferase-tagged AdV types in submerged 2D and organotypic 3D cell culture models. Transduction experiments revealed robust transduction of AdV type 5 (AdV5) in NAEPCs, which was associated with an increased uptake of AdV5 in the presence of HDM. In healthy and asthmatic NAEPCs exposed to HDM before infection, we observed a time- and dose-dependent increase of AdV5 uptake associated with upregulation of entry receptors for AdV5. Furthermore, electron microscopic and histologic analyses of 3D cell cultures revealed an impairment of the respiratory cilia after HDM exposition. This ex vivo pilot study shows the impact of AdV infection and HDM exposition in a primary cell culture model for asthma.
format Online
Article
Text
id pubmed-7600414
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76004142020-11-01 House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection Aydin, Malik Naumova, Ella A. Paulsen, Friedrich Zhang, Wenli Gopon, Felix Theis, Christian Lutz, Sören Ehrke-Schulz, Eric Arnold, Wolfgang H. Wirth, Stefan Ehrhardt, Anja Viruses Article Adenovirus (AdV) infections in the respiratory tract may cause asthma exacerbation and allergic predisposition, and the house dust mite (HDM) may aggravate virus-induced asthma exacerbations. However, the underlying mechanisms of whether and how AdV affects asthmatic patients remains unclear. To address this question, we investigated nasal epithelial cells (NAEPCs) derived from a pediatric exacerbation study cohort for experimental analyses. We analyzed twenty-one different green-fluorescent protein- and luciferase-tagged AdV types in submerged 2D and organotypic 3D cell culture models. Transduction experiments revealed robust transduction of AdV type 5 (AdV5) in NAEPCs, which was associated with an increased uptake of AdV5 in the presence of HDM. In healthy and asthmatic NAEPCs exposed to HDM before infection, we observed a time- and dose-dependent increase of AdV5 uptake associated with upregulation of entry receptors for AdV5. Furthermore, electron microscopic and histologic analyses of 3D cell cultures revealed an impairment of the respiratory cilia after HDM exposition. This ex vivo pilot study shows the impact of AdV infection and HDM exposition in a primary cell culture model for asthma. MDPI 2020-10-11 /pmc/articles/PMC7600414/ /pubmed/33050625 http://dx.doi.org/10.3390/v12101151 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aydin, Malik
Naumova, Ella A.
Paulsen, Friedrich
Zhang, Wenli
Gopon, Felix
Theis, Christian
Lutz, Sören
Ehrke-Schulz, Eric
Arnold, Wolfgang H.
Wirth, Stefan
Ehrhardt, Anja
House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection
title House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection
title_full House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection
title_fullStr House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection
title_full_unstemmed House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection
title_short House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection
title_sort house dust mite exposure causes increased susceptibility of nasal epithelial cells to adenovirus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600414/
https://www.ncbi.nlm.nih.gov/pubmed/33050625
http://dx.doi.org/10.3390/v12101151
work_keys_str_mv AT aydinmalik housedustmiteexposurecausesincreasedsusceptibilityofnasalepithelialcellstoadenovirusinfection
AT naumovaellaa housedustmiteexposurecausesincreasedsusceptibilityofnasalepithelialcellstoadenovirusinfection
AT paulsenfriedrich housedustmiteexposurecausesincreasedsusceptibilityofnasalepithelialcellstoadenovirusinfection
AT zhangwenli housedustmiteexposurecausesincreasedsusceptibilityofnasalepithelialcellstoadenovirusinfection
AT goponfelix housedustmiteexposurecausesincreasedsusceptibilityofnasalepithelialcellstoadenovirusinfection
AT theischristian housedustmiteexposurecausesincreasedsusceptibilityofnasalepithelialcellstoadenovirusinfection
AT lutzsoren housedustmiteexposurecausesincreasedsusceptibilityofnasalepithelialcellstoadenovirusinfection
AT ehrkeschulzeric housedustmiteexposurecausesincreasedsusceptibilityofnasalepithelialcellstoadenovirusinfection
AT arnoldwolfgangh housedustmiteexposurecausesincreasedsusceptibilityofnasalepithelialcellstoadenovirusinfection
AT wirthstefan housedustmiteexposurecausesincreasedsusceptibilityofnasalepithelialcellstoadenovirusinfection
AT ehrhardtanja housedustmiteexposurecausesincreasedsusceptibilityofnasalepithelialcellstoadenovirusinfection