Metarhizium Anisopliae Challenges Immunity and Demography of Plutella xylostella

SIMPLE SUMMARY: The diamondback moth, Plutella xylostella, is a destructive pest of cruciferous crops worldwide. Integrated pest management (IPM) strategies, largely involve the use chemical pesticides which are harmful for the environment and human health. In this study, the virulence of three species of entomopathogenic fungi were tested. Metarhizium anisopliae proved to be the most effective by killing more than 90% of the population. Based on which the fungus was selected to study the host-pathogen immune interactions. More precisely, after infection, superoxide dismutase (SOD) and phenoloxidase (PO), two major enzymes involved in immune response, were studied at different time points. The fungus gradually weakened the enzyme activities as the time progressed, indicating that physiological attributes of host were adversely affected. The expression of immune-related genes (Defensin, Spaetzle, Cecropin, Lysozyme, and Hemolin) varied on different time points. Moreover, the fungus negatively impacted the development of the host by reducing the life span and egg laying ability. Thus, M. anisopliae can become a potent prospect for the control of this pest. This information will also reinforce the development of policies for biocontrol-based pest management. ABSTRACT: Entomopathogenic fungi are naturally existing microbes, that can serve as a key regulator of insect pests in integrated pest management strategies. Besides having no hazardous effects on the environment, these entomopathogens are alternatives to synthetic insecticides that can control notorious insect-like Plutella xylostella, a destructive pest of cruciferous crops. Three different species of entomopathogenic fungi were evaluated before the selection (high larval mortality and least LC(50)) of Metarhizum anisopliae. The study was designed to investigate the mortality, development, and immune responses of P. xylostella when challenged with M. anisopliae, a naturally existing soil-borne entomopathogenic fungus. M. anisopliae resulted in high pest mortality by killing 93% of larvae. However, no statistically significant effect on hemocyte concentration was observed. The activity of enzymes (Phenoloxidase and Superoxide dismutase) and immune genes (Defensin, Spaetzle, Cecropin, Lysozyme, and Hemolin) did vary at different time points (24, 48, 72 and 96 h) after exposure to M. anisopliae. Disturbance in the biological cycles of P. xylostella was also detected, significantly shorter adult life span (8.11:6.87, M:F) and reduced fecundity (101 eggs/female) were observed along with disturbed larval and pupal duration. Results suggest that M. anisopliae can efficiently hinder the P. xylostella defense and developmental system, resulting in mortality and disturbed demography.