Cargando…

Running on Empty: A Metabolomics Approach to Investigating Changing Energy Metabolism during Fasted Exercise and Rest

Understanding the metabolic processes in energy metabolism, particularly during fasted exercise, is a growing area of research. Previous work has focused on measuring metabolites pre and post exercise. This can provide information about the final state of energy metabolism in the participants, but i...

Descripción completa

Detalles Bibliográficos
Autores principales: Blackburn, Gavin, Hay, Joshua, Skagen, Christine, Paul, Elizabeth, Achcar, Fiona, Wilson, John, Best, Cameron, Manson, Erin, Burgess, Karl, Barrett, Michael P., Gill, Jason M. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600507/
https://www.ncbi.nlm.nih.gov/pubmed/33050077
http://dx.doi.org/10.3390/metabo10100399
_version_ 1783603161104121856
author Blackburn, Gavin
Hay, Joshua
Skagen, Christine
Paul, Elizabeth
Achcar, Fiona
Wilson, John
Best, Cameron
Manson, Erin
Burgess, Karl
Barrett, Michael P.
Gill, Jason M. R.
author_facet Blackburn, Gavin
Hay, Joshua
Skagen, Christine
Paul, Elizabeth
Achcar, Fiona
Wilson, John
Best, Cameron
Manson, Erin
Burgess, Karl
Barrett, Michael P.
Gill, Jason M. R.
author_sort Blackburn, Gavin
collection PubMed
description Understanding the metabolic processes in energy metabolism, particularly during fasted exercise, is a growing area of research. Previous work has focused on measuring metabolites pre and post exercise. This can provide information about the final state of energy metabolism in the participants, but it does not show how these processes vary during the exercise and any subsequent post-exercise period. To address this, the work described here took fasted participants and subjected them to an exercise and rest protocol under laboratory settings, which allowed for breath and blood sampling both pre, during and post exercise. Analysis of the data produced from both the physiological measurements and the untargeted metabolomics measurements showed clear switching between glycolytic and ketolytic metabolism, with the liquid chromatography-mass spectrometry (LC-MS) data showing the separate stages of ketolytic metabolism, notably the transport, release and breakdown of long chain fatty acids. Several signals, putatively identified as short peptides, were observed to change in a pattern similar to that of the ketolytic metabolites. This work highlights the power of untargeted metabolomic methods as an investigative tool for exercise science, both to follow known processes in a more complete way and discover possible novel biomarkers.
format Online
Article
Text
id pubmed-7600507
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-76005072020-11-01 Running on Empty: A Metabolomics Approach to Investigating Changing Energy Metabolism during Fasted Exercise and Rest Blackburn, Gavin Hay, Joshua Skagen, Christine Paul, Elizabeth Achcar, Fiona Wilson, John Best, Cameron Manson, Erin Burgess, Karl Barrett, Michael P. Gill, Jason M. R. Metabolites Article Understanding the metabolic processes in energy metabolism, particularly during fasted exercise, is a growing area of research. Previous work has focused on measuring metabolites pre and post exercise. This can provide information about the final state of energy metabolism in the participants, but it does not show how these processes vary during the exercise and any subsequent post-exercise period. To address this, the work described here took fasted participants and subjected them to an exercise and rest protocol under laboratory settings, which allowed for breath and blood sampling both pre, during and post exercise. Analysis of the data produced from both the physiological measurements and the untargeted metabolomics measurements showed clear switching between glycolytic and ketolytic metabolism, with the liquid chromatography-mass spectrometry (LC-MS) data showing the separate stages of ketolytic metabolism, notably the transport, release and breakdown of long chain fatty acids. Several signals, putatively identified as short peptides, were observed to change in a pattern similar to that of the ketolytic metabolites. This work highlights the power of untargeted metabolomic methods as an investigative tool for exercise science, both to follow known processes in a more complete way and discover possible novel biomarkers. MDPI 2020-10-08 /pmc/articles/PMC7600507/ /pubmed/33050077 http://dx.doi.org/10.3390/metabo10100399 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Blackburn, Gavin
Hay, Joshua
Skagen, Christine
Paul, Elizabeth
Achcar, Fiona
Wilson, John
Best, Cameron
Manson, Erin
Burgess, Karl
Barrett, Michael P.
Gill, Jason M. R.
Running on Empty: A Metabolomics Approach to Investigating Changing Energy Metabolism during Fasted Exercise and Rest
title Running on Empty: A Metabolomics Approach to Investigating Changing Energy Metabolism during Fasted Exercise and Rest
title_full Running on Empty: A Metabolomics Approach to Investigating Changing Energy Metabolism during Fasted Exercise and Rest
title_fullStr Running on Empty: A Metabolomics Approach to Investigating Changing Energy Metabolism during Fasted Exercise and Rest
title_full_unstemmed Running on Empty: A Metabolomics Approach to Investigating Changing Energy Metabolism during Fasted Exercise and Rest
title_short Running on Empty: A Metabolomics Approach to Investigating Changing Energy Metabolism during Fasted Exercise and Rest
title_sort running on empty: a metabolomics approach to investigating changing energy metabolism during fasted exercise and rest
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600507/
https://www.ncbi.nlm.nih.gov/pubmed/33050077
http://dx.doi.org/10.3390/metabo10100399
work_keys_str_mv AT blackburngavin runningonemptyametabolomicsapproachtoinvestigatingchangingenergymetabolismduringfastedexerciseandrest
AT hayjoshua runningonemptyametabolomicsapproachtoinvestigatingchangingenergymetabolismduringfastedexerciseandrest
AT skagenchristine runningonemptyametabolomicsapproachtoinvestigatingchangingenergymetabolismduringfastedexerciseandrest
AT paulelizabeth runningonemptyametabolomicsapproachtoinvestigatingchangingenergymetabolismduringfastedexerciseandrest
AT achcarfiona runningonemptyametabolomicsapproachtoinvestigatingchangingenergymetabolismduringfastedexerciseandrest
AT wilsonjohn runningonemptyametabolomicsapproachtoinvestigatingchangingenergymetabolismduringfastedexerciseandrest
AT bestcameron runningonemptyametabolomicsapproachtoinvestigatingchangingenergymetabolismduringfastedexerciseandrest
AT mansonerin runningonemptyametabolomicsapproachtoinvestigatingchangingenergymetabolismduringfastedexerciseandrest
AT burgesskarl runningonemptyametabolomicsapproachtoinvestigatingchangingenergymetabolismduringfastedexerciseandrest
AT barrettmichaelp runningonemptyametabolomicsapproachtoinvestigatingchangingenergymetabolismduringfastedexerciseandrest
AT gilljasonmr runningonemptyametabolomicsapproachtoinvestigatingchangingenergymetabolismduringfastedexerciseandrest