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Procyanidin-Rich Extract from Grape Seeds as a Putative Tool against Helicobacter pylori
Strains of Helicobacter pylori (H. pylori) resistant to various antibiotics have increased in recent years. In this context, the search for new therapeutic approaches is crucial. The aim of the present study was to demonstrate the antibacterial activity of a procyanidin-rich extract obtained from fo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600706/ https://www.ncbi.nlm.nih.gov/pubmed/32993186 http://dx.doi.org/10.3390/foods9101370 |
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author | Silvan, Jose Manuel Gutiérrez-Docio, Alba Moreno-Fernandez, Silvia Alarcón-Cavero, Teresa Prodanov, Marin Martinez-Rodriguez, Adolfo J. |
author_facet | Silvan, Jose Manuel Gutiérrez-Docio, Alba Moreno-Fernandez, Silvia Alarcón-Cavero, Teresa Prodanov, Marin Martinez-Rodriguez, Adolfo J. |
author_sort | Silvan, Jose Manuel |
collection | PubMed |
description | Strains of Helicobacter pylori (H. pylori) resistant to various antibiotics have increased in recent years. In this context, the search for new therapeutic approaches is crucial. The aim of the present study was to demonstrate the antibacterial activity of a procyanidin-rich extract obtained from food-grade winery grape seeds against 14 H. pylori strains and elucidate its phenolic composition. Ten strains (71.4%) showed resistance to at least some of the tested antibiotics, while four isolates (28.6%) were susceptible to all antibiotics. Resistance to more than one class of antibiotics was observed in six strains (42.9%). The extract was able to inhibit the growth of all H. pylori strains in a range of a minimum inhibitory concentration (MIC) from 0.015 mg/mL to 0.125 mg/mL, confirming also the existence of a strain-dependent effect. The phenolic composition determined by reverse phase high pressure liquid chromatography, photodiode array, and mass spectrometry detection (RP-HPLC-PAD-MS) analysis revealed the presence of 43 individual compounds and allowed the quantification of 41 of them, including seven procyanidin tetramers, seven procyanidin pentamers, and six galloylated procyanidin dimers, trimers, and tetramers. The extract was composed mainly by catechin and procyanidin oligomers with a total amount of 5801 mg/100 g, which represent 92% of the total individual phenolic content. Among them, the most abundant were catechins (2047 mg/100 g), followed by procyanidin dimers (1550 mg/100 g), trimers (1176 mg/100 g), tetramers (436 mg/100 g), and pentamers (296 mg/100 g) that represent 35, 27, 20, 8, and 5%, respectively of the total flavanol constituents. The composition profile information may help to improve the production process of useful antibacterial extracts against H. pylori. |
format | Online Article Text |
id | pubmed-7600706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76007062020-11-01 Procyanidin-Rich Extract from Grape Seeds as a Putative Tool against Helicobacter pylori Silvan, Jose Manuel Gutiérrez-Docio, Alba Moreno-Fernandez, Silvia Alarcón-Cavero, Teresa Prodanov, Marin Martinez-Rodriguez, Adolfo J. Foods Article Strains of Helicobacter pylori (H. pylori) resistant to various antibiotics have increased in recent years. In this context, the search for new therapeutic approaches is crucial. The aim of the present study was to demonstrate the antibacterial activity of a procyanidin-rich extract obtained from food-grade winery grape seeds against 14 H. pylori strains and elucidate its phenolic composition. Ten strains (71.4%) showed resistance to at least some of the tested antibiotics, while four isolates (28.6%) were susceptible to all antibiotics. Resistance to more than one class of antibiotics was observed in six strains (42.9%). The extract was able to inhibit the growth of all H. pylori strains in a range of a minimum inhibitory concentration (MIC) from 0.015 mg/mL to 0.125 mg/mL, confirming also the existence of a strain-dependent effect. The phenolic composition determined by reverse phase high pressure liquid chromatography, photodiode array, and mass spectrometry detection (RP-HPLC-PAD-MS) analysis revealed the presence of 43 individual compounds and allowed the quantification of 41 of them, including seven procyanidin tetramers, seven procyanidin pentamers, and six galloylated procyanidin dimers, trimers, and tetramers. The extract was composed mainly by catechin and procyanidin oligomers with a total amount of 5801 mg/100 g, which represent 92% of the total individual phenolic content. Among them, the most abundant were catechins (2047 mg/100 g), followed by procyanidin dimers (1550 mg/100 g), trimers (1176 mg/100 g), tetramers (436 mg/100 g), and pentamers (296 mg/100 g) that represent 35, 27, 20, 8, and 5%, respectively of the total flavanol constituents. The composition profile information may help to improve the production process of useful antibacterial extracts against H. pylori. MDPI 2020-09-26 /pmc/articles/PMC7600706/ /pubmed/32993186 http://dx.doi.org/10.3390/foods9101370 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Silvan, Jose Manuel Gutiérrez-Docio, Alba Moreno-Fernandez, Silvia Alarcón-Cavero, Teresa Prodanov, Marin Martinez-Rodriguez, Adolfo J. Procyanidin-Rich Extract from Grape Seeds as a Putative Tool against Helicobacter pylori |
title | Procyanidin-Rich Extract from Grape Seeds as a Putative Tool against Helicobacter pylori |
title_full | Procyanidin-Rich Extract from Grape Seeds as a Putative Tool against Helicobacter pylori |
title_fullStr | Procyanidin-Rich Extract from Grape Seeds as a Putative Tool against Helicobacter pylori |
title_full_unstemmed | Procyanidin-Rich Extract from Grape Seeds as a Putative Tool against Helicobacter pylori |
title_short | Procyanidin-Rich Extract from Grape Seeds as a Putative Tool against Helicobacter pylori |
title_sort | procyanidin-rich extract from grape seeds as a putative tool against helicobacter pylori |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600706/ https://www.ncbi.nlm.nih.gov/pubmed/32993186 http://dx.doi.org/10.3390/foods9101370 |
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