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Morphological Description of the Early Events during the Invasion of Acanthamoeba castellanii Trophozoites in a Murine Model of Skin Irradiated under UV-B Light

Skin infections have been associated with Acanthamoeba, nevertheless the events during skin invasion and UV-B light effects on it are unknown. The early morphological events of Acanthamoeba castellanii skin invasion are shown in SKH-1 mice that were chronically UV-B light irradiated. Mice that devel...

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Autores principales: Hernández-Jasso, Mariana, Hernández-Martínez, Dolores, Avila-Acevedo, José Guillermo, Benítez-Flores, José del Carmen, Gallegos-Hernández, Isis Amara, García-Bores, Ana María, Espinosa-González, Adriana Montserrat, Villamar-Duque, Tomás Ernesto, Castelan-Ramírez, Ismael, González-Valle, María del Rosario, Omaña-Molina, Maritza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600863/
https://www.ncbi.nlm.nih.gov/pubmed/32992452
http://dx.doi.org/10.3390/pathogens9100794
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author Hernández-Jasso, Mariana
Hernández-Martínez, Dolores
Avila-Acevedo, José Guillermo
Benítez-Flores, José del Carmen
Gallegos-Hernández, Isis Amara
García-Bores, Ana María
Espinosa-González, Adriana Montserrat
Villamar-Duque, Tomás Ernesto
Castelan-Ramírez, Ismael
González-Valle, María del Rosario
Omaña-Molina, Maritza
author_facet Hernández-Jasso, Mariana
Hernández-Martínez, Dolores
Avila-Acevedo, José Guillermo
Benítez-Flores, José del Carmen
Gallegos-Hernández, Isis Amara
García-Bores, Ana María
Espinosa-González, Adriana Montserrat
Villamar-Duque, Tomás Ernesto
Castelan-Ramírez, Ismael
González-Valle, María del Rosario
Omaña-Molina, Maritza
author_sort Hernández-Jasso, Mariana
collection PubMed
description Skin infections have been associated with Acanthamoeba, nevertheless the events during skin invasion and UV-B light effects on it are unknown. The early morphological events of Acanthamoeba castellanii skin invasion are shown in SKH-1 mice that were chronically UV-B light irradiated. Mice that developed skin lesions (group 1) were topical and intradermally inoculated with A. castellanii trophozoites and sacrificed 48 h or 18 days later. Mice that showed no skin lesions (group 2) were intradermally inoculated and sacrificed 24, 48 or 72 h later. Mice ventral areas were considered controls with and without trophozoites intradermally inoculated. Skin samples were processed by histological and immunohistochemistry techniques. In group 1, trophozoites were immunolocalized in dermal areas, hair cysts, sebaceous glands, and blood vessels, and collagen degradation was observed. One of these mice shown trophozoites in the spleen, liver, and brain. In group 2, few trophozoites nearby collagenolytic activity zones were observed. In control samples, nor histological damage and no trophozoites were observed. Adherence and collagenolytic activity by A. castellanii were corroborated in vitro. We can infer that UV-B light irradiated skin could favor A. castellanii invasiveness causing damage in sites as far away as the brain, confirming the invasive capacity and pathogenic potential of these amphizoic amoebae.
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spelling pubmed-76008632020-11-01 Morphological Description of the Early Events during the Invasion of Acanthamoeba castellanii Trophozoites in a Murine Model of Skin Irradiated under UV-B Light Hernández-Jasso, Mariana Hernández-Martínez, Dolores Avila-Acevedo, José Guillermo Benítez-Flores, José del Carmen Gallegos-Hernández, Isis Amara García-Bores, Ana María Espinosa-González, Adriana Montserrat Villamar-Duque, Tomás Ernesto Castelan-Ramírez, Ismael González-Valle, María del Rosario Omaña-Molina, Maritza Pathogens Article Skin infections have been associated with Acanthamoeba, nevertheless the events during skin invasion and UV-B light effects on it are unknown. The early morphological events of Acanthamoeba castellanii skin invasion are shown in SKH-1 mice that were chronically UV-B light irradiated. Mice that developed skin lesions (group 1) were topical and intradermally inoculated with A. castellanii trophozoites and sacrificed 48 h or 18 days later. Mice that showed no skin lesions (group 2) were intradermally inoculated and sacrificed 24, 48 or 72 h later. Mice ventral areas were considered controls with and without trophozoites intradermally inoculated. Skin samples were processed by histological and immunohistochemistry techniques. In group 1, trophozoites were immunolocalized in dermal areas, hair cysts, sebaceous glands, and blood vessels, and collagen degradation was observed. One of these mice shown trophozoites in the spleen, liver, and brain. In group 2, few trophozoites nearby collagenolytic activity zones were observed. In control samples, nor histological damage and no trophozoites were observed. Adherence and collagenolytic activity by A. castellanii were corroborated in vitro. We can infer that UV-B light irradiated skin could favor A. castellanii invasiveness causing damage in sites as far away as the brain, confirming the invasive capacity and pathogenic potential of these amphizoic amoebae. MDPI 2020-09-27 /pmc/articles/PMC7600863/ /pubmed/32992452 http://dx.doi.org/10.3390/pathogens9100794 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hernández-Jasso, Mariana
Hernández-Martínez, Dolores
Avila-Acevedo, José Guillermo
Benítez-Flores, José del Carmen
Gallegos-Hernández, Isis Amara
García-Bores, Ana María
Espinosa-González, Adriana Montserrat
Villamar-Duque, Tomás Ernesto
Castelan-Ramírez, Ismael
González-Valle, María del Rosario
Omaña-Molina, Maritza
Morphological Description of the Early Events during the Invasion of Acanthamoeba castellanii Trophozoites in a Murine Model of Skin Irradiated under UV-B Light
title Morphological Description of the Early Events during the Invasion of Acanthamoeba castellanii Trophozoites in a Murine Model of Skin Irradiated under UV-B Light
title_full Morphological Description of the Early Events during the Invasion of Acanthamoeba castellanii Trophozoites in a Murine Model of Skin Irradiated under UV-B Light
title_fullStr Morphological Description of the Early Events during the Invasion of Acanthamoeba castellanii Trophozoites in a Murine Model of Skin Irradiated under UV-B Light
title_full_unstemmed Morphological Description of the Early Events during the Invasion of Acanthamoeba castellanii Trophozoites in a Murine Model of Skin Irradiated under UV-B Light
title_short Morphological Description of the Early Events during the Invasion of Acanthamoeba castellanii Trophozoites in a Murine Model of Skin Irradiated under UV-B Light
title_sort morphological description of the early events during the invasion of acanthamoeba castellanii trophozoites in a murine model of skin irradiated under uv-b light
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600863/
https://www.ncbi.nlm.nih.gov/pubmed/32992452
http://dx.doi.org/10.3390/pathogens9100794
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