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Characteristics of the IVF Cycle that Contribute to the Incidence of Mosaicism

Highly sensitive next-generation sequencing (NGS) platforms applied to preimplantation genetic testing for aneuploidy (PGT-A) allow the classification of mosaicism in trophectoderm biopsies. However, the incidence of mosaicism reported by these tests can be affected by a wide number of analytical, b...

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Autores principales: Rodrigo, Lorena, Clemente-Císcar, Mónica, Campos-Galindo, Inmaculada, Peinado, Vanessa, Simón, Carlos, Rubio, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600993/
https://www.ncbi.nlm.nih.gov/pubmed/33007801
http://dx.doi.org/10.3390/genes11101151
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author Rodrigo, Lorena
Clemente-Císcar, Mónica
Campos-Galindo, Inmaculada
Peinado, Vanessa
Simón, Carlos
Rubio, Carmen
author_facet Rodrigo, Lorena
Clemente-Císcar, Mónica
Campos-Galindo, Inmaculada
Peinado, Vanessa
Simón, Carlos
Rubio, Carmen
author_sort Rodrigo, Lorena
collection PubMed
description Highly sensitive next-generation sequencing (NGS) platforms applied to preimplantation genetic testing for aneuploidy (PGT-A) allow the classification of mosaicism in trophectoderm biopsies. However, the incidence of mosaicism reported by these tests can be affected by a wide number of analytical, biological, and clinical factors. With the use of a proprietary algorithm for automated diagnosis of aneuploidy and mosaicism, we retrospectively analyzed a large series of 115,368 trophectoderm biopsies from 27,436 PGT-A cycles to determine whether certain biological factors and in vitro fertilization (IVF) practices influence the incidence of overall aneuploidy, whole uniform aneuploidy, mosaicism, and TE biopsies with only segmental aneuploidy. Older female and male patients showed higher rates of high-mosaic degree and whole uniform aneuploidies and severe oligozoospermic patients had higher rates of mosaicism and only segmental aneuploidies. Logistic regression analysis identified a positive effect of female age but a negative effect of embryo vitrification on the incidence of overall aneuploid embryos. Female age increased whole uniform aneuploidy rates but decreased only segmental aneuploidy and mosaicism, mainly low-mosaics. Conversely, higher ovarian response decreased whole uniform aneuploidy rates but increased only segmental aneuploidies. Finally, embryo vitrification decreased whole uniform aneuploidy rates but increased mosaicism, mainly low-mosaics, compared to PGT-A cycles with fresh oocytes. These results could be useful for clinician’s management of the IVF cycles.
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spelling pubmed-76009932020-11-01 Characteristics of the IVF Cycle that Contribute to the Incidence of Mosaicism Rodrigo, Lorena Clemente-Císcar, Mónica Campos-Galindo, Inmaculada Peinado, Vanessa Simón, Carlos Rubio, Carmen Genes (Basel) Article Highly sensitive next-generation sequencing (NGS) platforms applied to preimplantation genetic testing for aneuploidy (PGT-A) allow the classification of mosaicism in trophectoderm biopsies. However, the incidence of mosaicism reported by these tests can be affected by a wide number of analytical, biological, and clinical factors. With the use of a proprietary algorithm for automated diagnosis of aneuploidy and mosaicism, we retrospectively analyzed a large series of 115,368 trophectoderm biopsies from 27,436 PGT-A cycles to determine whether certain biological factors and in vitro fertilization (IVF) practices influence the incidence of overall aneuploidy, whole uniform aneuploidy, mosaicism, and TE biopsies with only segmental aneuploidy. Older female and male patients showed higher rates of high-mosaic degree and whole uniform aneuploidies and severe oligozoospermic patients had higher rates of mosaicism and only segmental aneuploidies. Logistic regression analysis identified a positive effect of female age but a negative effect of embryo vitrification on the incidence of overall aneuploid embryos. Female age increased whole uniform aneuploidy rates but decreased only segmental aneuploidy and mosaicism, mainly low-mosaics. Conversely, higher ovarian response decreased whole uniform aneuploidy rates but increased only segmental aneuploidies. Finally, embryo vitrification decreased whole uniform aneuploidy rates but increased mosaicism, mainly low-mosaics, compared to PGT-A cycles with fresh oocytes. These results could be useful for clinician’s management of the IVF cycles. MDPI 2020-09-30 /pmc/articles/PMC7600993/ /pubmed/33007801 http://dx.doi.org/10.3390/genes11101151 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rodrigo, Lorena
Clemente-Císcar, Mónica
Campos-Galindo, Inmaculada
Peinado, Vanessa
Simón, Carlos
Rubio, Carmen
Characteristics of the IVF Cycle that Contribute to the Incidence of Mosaicism
title Characteristics of the IVF Cycle that Contribute to the Incidence of Mosaicism
title_full Characteristics of the IVF Cycle that Contribute to the Incidence of Mosaicism
title_fullStr Characteristics of the IVF Cycle that Contribute to the Incidence of Mosaicism
title_full_unstemmed Characteristics of the IVF Cycle that Contribute to the Incidence of Mosaicism
title_short Characteristics of the IVF Cycle that Contribute to the Incidence of Mosaicism
title_sort characteristics of the ivf cycle that contribute to the incidence of mosaicism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7600993/
https://www.ncbi.nlm.nih.gov/pubmed/33007801
http://dx.doi.org/10.3390/genes11101151
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