Kineococcus vitellinus sp. nov., Kineococcus indalonis sp. nov. and Kineococcus siccus sp. nov., Isolated Nearby the Tabernas Desert (Almería, Spain)

Three novel Gram-positive, aerobic, chemoheterotrophic, motile, non-endospore-forming, orange-pigmented bacteria designated strains T13(T), T90(T) and R8(T) were isolated from the Tabernas Desert biocrust (Almería, Spain). Cells of the three strains were coccus-shaped and occurred singly, in pairs or clusters. The three strains were oxidase-negative and catalase-positive, and showed a mesophilic, neutrophilic and non-halophilic metabolism. Based on the 16S rRNA gene sequences, the closest neighbours of strains T13(T), T90(T) and R8(T) were Kineococcus aurantiacus IFO 15268(T), Kineococcus gypseus YIM 121300(T) and Kineococcus radiotolerans SRS 30216(T) (98.5%, 97.1% and 97.9% gene sequence similarity, respectively). The genomes were sequenced, and have been deposited in the GenBank/EMBL/DDBJ databases under the accession numbers JAAALL000000000, JAAALM000000000 and JAAALN000000000, respectively, for strains T13(T), T90(T) and R8(T). The average nucleotide identity (ANIb) and digital DNA-DNA hybridization (dDDH) values confirmed their adscription to three new species within the genus Kineococcus. The genomic G + C content of strains T13(T), T90(T) and R8(T) ranged from 75.1% to 76.3%. The predominant fatty acid of all three strains was anteiso-C(15:0). According to a polyphasic study, strains T13(T), T90(T) and R8(T) are representatives of three new species in the genus Kineococcus, for which names Kineococcus vitellinus sp. nov. (type strain T13(T) = CECT 9936(T) = DSM 110024(T)), Kineococcus indalonis sp. nov. (type strain T90(T) = CECT 9938(T) = DSM 110026(T)) and Kineococcus siccus sp. nov. (type strain R8(T) = CECT 9937(T) = DSM 110025(T)) are proposed.