Cargando…
Repositioning N-Acetylcysteine (NAC): NAC-Loaded Electrospun Drug Delivery Scaffolding for Potential Neural Tissue Engineering Application
Traumatic brain injury (TBI) presents a serious challenge for modern medicine due to the poor regenerative capabilities of the brain, complex pathophysiology, and lack of effective treatment for TBI to date. Tissue-engineered scaffolds have shown some experimental success in vivo; unfortunately, non...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601117/ https://www.ncbi.nlm.nih.gov/pubmed/33007830 http://dx.doi.org/10.3390/pharmaceutics12100934 |
_version_ | 1783603325910908928 |
---|---|
author | Mahumane, Gillian D. Kumar, Pradeep Pillay, Viness Choonara, Yahya E. |
author_facet | Mahumane, Gillian D. Kumar, Pradeep Pillay, Viness Choonara, Yahya E. |
author_sort | Mahumane, Gillian D. |
collection | PubMed |
description | Traumatic brain injury (TBI) presents a serious challenge for modern medicine due to the poor regenerative capabilities of the brain, complex pathophysiology, and lack of effective treatment for TBI to date. Tissue-engineered scaffolds have shown some experimental success in vivo; unfortunately, none have yielded consummate results of clinical efficacy. N-acetylcysteine has shown neuroprotective potential. To this end, we developed a N-acetylcysteine (NAC)-loaded poly(lactic-co-glycolic acid) (PLGA) electrospun system for potential neural tissue application for TBI. Scanning electron microscopy showed nanofiber diameters ranging 72–542 nm and 124–592 nm for NAC-free and NAC-loaded PLGA nanofibers, respectively. NAC loading was obtained at 28%, and drug entrapment efficacy was obtained at 84%. A biphasic NAC release pattern that featured an initial burst release (13.9%) stage and a later sustained release stage was noted, thus enabling the prolonged replenishing of NAC and drastically improving cell viability and proliferation. This was evidenced by a significantly higher cell viability and proliferation on NAC-loaded nanofibers for rat pheochromocytoma (PC12) and human glioblastoma multiform (A172) cell lines in comparison to PLGA-only nanofibers. The increased cell viability and cell proliferation on NAC-loaded nanofiber substantiates for the repositioning of NAC as a pharmacological agent in neural tissue regeneration applications. |
format | Online Article Text |
id | pubmed-7601117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76011172020-11-01 Repositioning N-Acetylcysteine (NAC): NAC-Loaded Electrospun Drug Delivery Scaffolding for Potential Neural Tissue Engineering Application Mahumane, Gillian D. Kumar, Pradeep Pillay, Viness Choonara, Yahya E. Pharmaceutics Article Traumatic brain injury (TBI) presents a serious challenge for modern medicine due to the poor regenerative capabilities of the brain, complex pathophysiology, and lack of effective treatment for TBI to date. Tissue-engineered scaffolds have shown some experimental success in vivo; unfortunately, none have yielded consummate results of clinical efficacy. N-acetylcysteine has shown neuroprotective potential. To this end, we developed a N-acetylcysteine (NAC)-loaded poly(lactic-co-glycolic acid) (PLGA) electrospun system for potential neural tissue application for TBI. Scanning electron microscopy showed nanofiber diameters ranging 72–542 nm and 124–592 nm for NAC-free and NAC-loaded PLGA nanofibers, respectively. NAC loading was obtained at 28%, and drug entrapment efficacy was obtained at 84%. A biphasic NAC release pattern that featured an initial burst release (13.9%) stage and a later sustained release stage was noted, thus enabling the prolonged replenishing of NAC and drastically improving cell viability and proliferation. This was evidenced by a significantly higher cell viability and proliferation on NAC-loaded nanofibers for rat pheochromocytoma (PC12) and human glioblastoma multiform (A172) cell lines in comparison to PLGA-only nanofibers. The increased cell viability and cell proliferation on NAC-loaded nanofiber substantiates for the repositioning of NAC as a pharmacological agent in neural tissue regeneration applications. MDPI 2020-09-30 /pmc/articles/PMC7601117/ /pubmed/33007830 http://dx.doi.org/10.3390/pharmaceutics12100934 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mahumane, Gillian D. Kumar, Pradeep Pillay, Viness Choonara, Yahya E. Repositioning N-Acetylcysteine (NAC): NAC-Loaded Electrospun Drug Delivery Scaffolding for Potential Neural Tissue Engineering Application |
title | Repositioning N-Acetylcysteine (NAC): NAC-Loaded Electrospun Drug Delivery Scaffolding for Potential Neural Tissue Engineering Application |
title_full | Repositioning N-Acetylcysteine (NAC): NAC-Loaded Electrospun Drug Delivery Scaffolding for Potential Neural Tissue Engineering Application |
title_fullStr | Repositioning N-Acetylcysteine (NAC): NAC-Loaded Electrospun Drug Delivery Scaffolding for Potential Neural Tissue Engineering Application |
title_full_unstemmed | Repositioning N-Acetylcysteine (NAC): NAC-Loaded Electrospun Drug Delivery Scaffolding for Potential Neural Tissue Engineering Application |
title_short | Repositioning N-Acetylcysteine (NAC): NAC-Loaded Electrospun Drug Delivery Scaffolding for Potential Neural Tissue Engineering Application |
title_sort | repositioning n-acetylcysteine (nac): nac-loaded electrospun drug delivery scaffolding for potential neural tissue engineering application |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601117/ https://www.ncbi.nlm.nih.gov/pubmed/33007830 http://dx.doi.org/10.3390/pharmaceutics12100934 |
work_keys_str_mv | AT mahumanegilliand repositioningnacetylcysteinenacnacloadedelectrospundrugdeliveryscaffoldingforpotentialneuraltissueengineeringapplication AT kumarpradeep repositioningnacetylcysteinenacnacloadedelectrospundrugdeliveryscaffoldingforpotentialneuraltissueengineeringapplication AT pillayviness repositioningnacetylcysteinenacnacloadedelectrospundrugdeliveryscaffoldingforpotentialneuraltissueengineeringapplication AT choonarayahyae repositioningnacetylcysteinenacnacloadedelectrospundrugdeliveryscaffoldingforpotentialneuraltissueengineeringapplication |