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The Dynamic Proteome of Oligodendrocyte Lineage Differentiation Features Planar Cell Polarity and Macroautophagy Pathways

BACKGROUND: Generation of oligodendrocytes is a sophisticated multistep process, the mechanistic underpinnings of which are not fully understood and demand further investigation. To systematically profile proteome dynamics during human embryonic stem cell differentiation into oligodendrocytes, we ap...

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Autores principales: Pooyan, Paria, Karamzadeh, Razieh, Mirzaei, Mehdi, Meyfour, Anna, Amirkhan, Ardeshir, Wu, Yunqi, Gupta, Vivek, Baharvand, Hossein, Javan, Mohammad, Salekdeh, Ghasem Hosseini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601170/
https://www.ncbi.nlm.nih.gov/pubmed/33128372
http://dx.doi.org/10.1093/gigascience/giaa116
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author Pooyan, Paria
Karamzadeh, Razieh
Mirzaei, Mehdi
Meyfour, Anna
Amirkhan, Ardeshir
Wu, Yunqi
Gupta, Vivek
Baharvand, Hossein
Javan, Mohammad
Salekdeh, Ghasem Hosseini
author_facet Pooyan, Paria
Karamzadeh, Razieh
Mirzaei, Mehdi
Meyfour, Anna
Amirkhan, Ardeshir
Wu, Yunqi
Gupta, Vivek
Baharvand, Hossein
Javan, Mohammad
Salekdeh, Ghasem Hosseini
author_sort Pooyan, Paria
collection PubMed
description BACKGROUND: Generation of oligodendrocytes is a sophisticated multistep process, the mechanistic underpinnings of which are not fully understood and demand further investigation. To systematically profile proteome dynamics during human embryonic stem cell differentiation into oligodendrocytes, we applied in-depth quantitative proteomics at different developmental stages and monitored changes in protein abundance using a multiplexed tandem mass tag-based proteomics approach. FINDINGS: Our proteome data provided a comprehensive protein expression profile that highlighted specific expression clusters based on the protein abundances over the course of human oligodendrocyte lineage differentiation. We identified the eminence of the planar cell polarity signalling and autophagy (particularly macroautophagy) in the progression of oligodendrocyte lineage differentiation—the cooperation of which is assisted by 106 and 77 proteins, respectively, that showed significant expression changes in this differentiation process. Furthermore, differentially expressed protein analysis of the proteome profile of oligodendrocyte lineage cells revealed 378 proteins that were specifically upregulated only in 1 differentiation stage. In addition, comparative pairwise analysis of differentiation stages demonstrated that abundances of 352 proteins differentially changed between consecutive differentiation time points. CONCLUSIONS: Our study provides a comprehensive systematic proteomics profile of oligodendrocyte lineage cells that can serve as a resource for identifying novel biomarkers from these cells and for indicating numerous proteins that may contribute to regulating the development of myelinating oligodendrocytes and other cells of oligodendrocyte lineage. We showed the importance of planar cell polarity signalling in oligodendrocyte lineage differentiation and revealed the autophagy-related proteins that participate in oligodendrocyte lineage differentiation.
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spelling pubmed-76011702020-11-04 The Dynamic Proteome of Oligodendrocyte Lineage Differentiation Features Planar Cell Polarity and Macroautophagy Pathways Pooyan, Paria Karamzadeh, Razieh Mirzaei, Mehdi Meyfour, Anna Amirkhan, Ardeshir Wu, Yunqi Gupta, Vivek Baharvand, Hossein Javan, Mohammad Salekdeh, Ghasem Hosseini Gigascience Data Note BACKGROUND: Generation of oligodendrocytes is a sophisticated multistep process, the mechanistic underpinnings of which are not fully understood and demand further investigation. To systematically profile proteome dynamics during human embryonic stem cell differentiation into oligodendrocytes, we applied in-depth quantitative proteomics at different developmental stages and monitored changes in protein abundance using a multiplexed tandem mass tag-based proteomics approach. FINDINGS: Our proteome data provided a comprehensive protein expression profile that highlighted specific expression clusters based on the protein abundances over the course of human oligodendrocyte lineage differentiation. We identified the eminence of the planar cell polarity signalling and autophagy (particularly macroautophagy) in the progression of oligodendrocyte lineage differentiation—the cooperation of which is assisted by 106 and 77 proteins, respectively, that showed significant expression changes in this differentiation process. Furthermore, differentially expressed protein analysis of the proteome profile of oligodendrocyte lineage cells revealed 378 proteins that were specifically upregulated only in 1 differentiation stage. In addition, comparative pairwise analysis of differentiation stages demonstrated that abundances of 352 proteins differentially changed between consecutive differentiation time points. CONCLUSIONS: Our study provides a comprehensive systematic proteomics profile of oligodendrocyte lineage cells that can serve as a resource for identifying novel biomarkers from these cells and for indicating numerous proteins that may contribute to regulating the development of myelinating oligodendrocytes and other cells of oligodendrocyte lineage. We showed the importance of planar cell polarity signalling in oligodendrocyte lineage differentiation and revealed the autophagy-related proteins that participate in oligodendrocyte lineage differentiation. Oxford University Press 2020-10-31 /pmc/articles/PMC7601170/ /pubmed/33128372 http://dx.doi.org/10.1093/gigascience/giaa116 Text en © The Author(s) 2020. Published by Oxford University Press GigaScience. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Data Note
Pooyan, Paria
Karamzadeh, Razieh
Mirzaei, Mehdi
Meyfour, Anna
Amirkhan, Ardeshir
Wu, Yunqi
Gupta, Vivek
Baharvand, Hossein
Javan, Mohammad
Salekdeh, Ghasem Hosseini
The Dynamic Proteome of Oligodendrocyte Lineage Differentiation Features Planar Cell Polarity and Macroautophagy Pathways
title The Dynamic Proteome of Oligodendrocyte Lineage Differentiation Features Planar Cell Polarity and Macroautophagy Pathways
title_full The Dynamic Proteome of Oligodendrocyte Lineage Differentiation Features Planar Cell Polarity and Macroautophagy Pathways
title_fullStr The Dynamic Proteome of Oligodendrocyte Lineage Differentiation Features Planar Cell Polarity and Macroautophagy Pathways
title_full_unstemmed The Dynamic Proteome of Oligodendrocyte Lineage Differentiation Features Planar Cell Polarity and Macroautophagy Pathways
title_short The Dynamic Proteome of Oligodendrocyte Lineage Differentiation Features Planar Cell Polarity and Macroautophagy Pathways
title_sort dynamic proteome of oligodendrocyte lineage differentiation features planar cell polarity and macroautophagy pathways
topic Data Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601170/
https://www.ncbi.nlm.nih.gov/pubmed/33128372
http://dx.doi.org/10.1093/gigascience/giaa116
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