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(64)Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis

Macrophages play a key role in the inflammatory response in Lyme arthritis (LA) and could be a target for diagnosing and monitoring active Borrelia burgdorferi sensu lato (Bb) infection. Therefore, we evaluated the potential of macrophage imaging using (64)Cu-DOTATATE PET/CT for detection of Bb acti...

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Autores principales: Skovsbo Clausen, Anne, Ørbæk, Mathilde, Renee Pedersen, Regitze, Oestrup Jensen, Peter, Lebech, Anne-Mette, Kjaer, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601205/
https://www.ncbi.nlm.nih.gov/pubmed/33036200
http://dx.doi.org/10.3390/diagnostics10100790
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author Skovsbo Clausen, Anne
Ørbæk, Mathilde
Renee Pedersen, Regitze
Oestrup Jensen, Peter
Lebech, Anne-Mette
Kjaer, Andreas
author_facet Skovsbo Clausen, Anne
Ørbæk, Mathilde
Renee Pedersen, Regitze
Oestrup Jensen, Peter
Lebech, Anne-Mette
Kjaer, Andreas
author_sort Skovsbo Clausen, Anne
collection PubMed
description Macrophages play a key role in the inflammatory response in Lyme arthritis (LA) and could be a target for diagnosing and monitoring active Borrelia burgdorferi sensu lato (Bb) infection. Therefore, we evaluated the potential of macrophage imaging using (64)Cu-DOTATATE PET/CT for detection of Bb activity in a murine model of LA. LA was established in C3H/HeNRj mice infected with Bb B31 strain ML23 pBBE22luc. Bioluminescence imaging was performed to detect migration of spirochetes and inflammatory phagocytes to the joints. Three weeks post-infection (64)Cu-DOTATATE PET/CT imaging was performed at an early (3 h) and late (48 h) time point. Plasma levels of a systemic macrophage marker in plasma CD163 were measured. (64)Cu-DOTATATE uptake in infected joints was increased at the early (p < 0.0001) and late time points (p = 0.0005) compared with uptake in non-infected controls. No significant difference in plasma levels of CD163 was measured. (64)Cu-DOTATATE PET allows for in vivo detection and quantification of LA locally in the joints through non-invasive visualization of macrophages. In contrast, measurement of a systemic macrophage marker in plasma, CD163, did not allow to detect disease. We suggest that (64)Cu-DOTATATE PET could become a valuable diagnostic tool for in situ detection of Bb infection-related inflammation.
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spelling pubmed-76012052020-11-01 (64)Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis Skovsbo Clausen, Anne Ørbæk, Mathilde Renee Pedersen, Regitze Oestrup Jensen, Peter Lebech, Anne-Mette Kjaer, Andreas Diagnostics (Basel) Article Macrophages play a key role in the inflammatory response in Lyme arthritis (LA) and could be a target for diagnosing and monitoring active Borrelia burgdorferi sensu lato (Bb) infection. Therefore, we evaluated the potential of macrophage imaging using (64)Cu-DOTATATE PET/CT for detection of Bb activity in a murine model of LA. LA was established in C3H/HeNRj mice infected with Bb B31 strain ML23 pBBE22luc. Bioluminescence imaging was performed to detect migration of spirochetes and inflammatory phagocytes to the joints. Three weeks post-infection (64)Cu-DOTATATE PET/CT imaging was performed at an early (3 h) and late (48 h) time point. Plasma levels of a systemic macrophage marker in plasma CD163 were measured. (64)Cu-DOTATATE uptake in infected joints was increased at the early (p < 0.0001) and late time points (p = 0.0005) compared with uptake in non-infected controls. No significant difference in plasma levels of CD163 was measured. (64)Cu-DOTATATE PET allows for in vivo detection and quantification of LA locally in the joints through non-invasive visualization of macrophages. In contrast, measurement of a systemic macrophage marker in plasma, CD163, did not allow to detect disease. We suggest that (64)Cu-DOTATATE PET could become a valuable diagnostic tool for in situ detection of Bb infection-related inflammation. MDPI 2020-10-06 /pmc/articles/PMC7601205/ /pubmed/33036200 http://dx.doi.org/10.3390/diagnostics10100790 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Skovsbo Clausen, Anne
Ørbæk, Mathilde
Renee Pedersen, Regitze
Oestrup Jensen, Peter
Lebech, Anne-Mette
Kjaer, Andreas
(64)Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis
title (64)Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis
title_full (64)Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis
title_fullStr (64)Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis
title_full_unstemmed (64)Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis
title_short (64)Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis
title_sort (64)cu-dotatate positron emission tomography (pet) of borrelia burgdorferi infection: in vivo imaging of macrophages in experimental model of lyme arthritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601205/
https://www.ncbi.nlm.nih.gov/pubmed/33036200
http://dx.doi.org/10.3390/diagnostics10100790
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