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Plasma Long Noncoding RNA LeXis is a Potential Diagnostic Marker for Non-Alcoholic Steatohepatitis
Non-invasive diagnostic markers are needed to ease the diagnosis of non-alcoholic steatohepatitis (NASH) among patients with non-alcoholic fatty liver disease (NAFLD). The long noncoding RNA (lncRNA) LeXis is related to cholesterol metabolism and hepatic steatosis in mice, and its batch genome conve...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601228/ https://www.ncbi.nlm.nih.gov/pubmed/33022942 http://dx.doi.org/10.3390/life10100230 |
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author | Park, Jung Gil Kim, Gyeonghwa Jang, Se Young Lee, Yu Rim Lee, Eunhye Lee, Hye Won Han, Man-Hoon Chun, Jae Min Han, Young Seok Yoon, Jun Sik Kang, Min Kyu Kweon, Young Oh Tak, Won Young Park, Soo Young Hur, Keun |
author_facet | Park, Jung Gil Kim, Gyeonghwa Jang, Se Young Lee, Yu Rim Lee, Eunhye Lee, Hye Won Han, Man-Hoon Chun, Jae Min Han, Young Seok Yoon, Jun Sik Kang, Min Kyu Kweon, Young Oh Tak, Won Young Park, Soo Young Hur, Keun |
author_sort | Park, Jung Gil |
collection | PubMed |
description | Non-invasive diagnostic markers are needed to ease the diagnosis of non-alcoholic steatohepatitis (NASH) among patients with non-alcoholic fatty liver disease (NAFLD). The long noncoding RNA (lncRNA) LeXis is related to cholesterol metabolism and hepatic steatosis in mice, and its batch genome conversion in humans is TCONS_00016452. Here, we aimed to evaluate the potential of lncRNA LeXis as a non-invasive diagnostic marker for NASH. We analyzed a total of 44 NAFLD patients whose diagnosis was confirmed by a pathologist through analysis of a percutaneous liver biopsy. The expression of LeXis in the plasma of NAFLD patients with and without NASH was compared using quantitative real-time polymerase chain reaction. The expression of plasma LeXis was significantly higher in patients with NASH than in those with NAFL (8.2 (5.0–14.9); 4.6 (4.0–6.6), p = 0.025). The area under the receiver operating characteristic curve was 0.743 (95% CI 0.590–0.895, p < 0.001), and a sensitivity of 54.3% and specificity of 100% could be achieved for NASH diagnosis. Low LeXis was independently associated with NASH diagnosis in patients with NAFLD (p = 0.0349, odds ratio = 22.19 (5% CI, 1.25–395.22)). Therefore, circulating lncRNA LeXis could be a potential non-invasive diagnostic biomarker for NASH. |
format | Online Article Text |
id | pubmed-7601228 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-76012282020-11-01 Plasma Long Noncoding RNA LeXis is a Potential Diagnostic Marker for Non-Alcoholic Steatohepatitis Park, Jung Gil Kim, Gyeonghwa Jang, Se Young Lee, Yu Rim Lee, Eunhye Lee, Hye Won Han, Man-Hoon Chun, Jae Min Han, Young Seok Yoon, Jun Sik Kang, Min Kyu Kweon, Young Oh Tak, Won Young Park, Soo Young Hur, Keun Life (Basel) Article Non-invasive diagnostic markers are needed to ease the diagnosis of non-alcoholic steatohepatitis (NASH) among patients with non-alcoholic fatty liver disease (NAFLD). The long noncoding RNA (lncRNA) LeXis is related to cholesterol metabolism and hepatic steatosis in mice, and its batch genome conversion in humans is TCONS_00016452. Here, we aimed to evaluate the potential of lncRNA LeXis as a non-invasive diagnostic marker for NASH. We analyzed a total of 44 NAFLD patients whose diagnosis was confirmed by a pathologist through analysis of a percutaneous liver biopsy. The expression of LeXis in the plasma of NAFLD patients with and without NASH was compared using quantitative real-time polymerase chain reaction. The expression of plasma LeXis was significantly higher in patients with NASH than in those with NAFL (8.2 (5.0–14.9); 4.6 (4.0–6.6), p = 0.025). The area under the receiver operating characteristic curve was 0.743 (95% CI 0.590–0.895, p < 0.001), and a sensitivity of 54.3% and specificity of 100% could be achieved for NASH diagnosis. Low LeXis was independently associated with NASH diagnosis in patients with NAFLD (p = 0.0349, odds ratio = 22.19 (5% CI, 1.25–395.22)). Therefore, circulating lncRNA LeXis could be a potential non-invasive diagnostic biomarker for NASH. MDPI 2020-10-03 /pmc/articles/PMC7601228/ /pubmed/33022942 http://dx.doi.org/10.3390/life10100230 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Park, Jung Gil Kim, Gyeonghwa Jang, Se Young Lee, Yu Rim Lee, Eunhye Lee, Hye Won Han, Man-Hoon Chun, Jae Min Han, Young Seok Yoon, Jun Sik Kang, Min Kyu Kweon, Young Oh Tak, Won Young Park, Soo Young Hur, Keun Plasma Long Noncoding RNA LeXis is a Potential Diagnostic Marker for Non-Alcoholic Steatohepatitis |
title | Plasma Long Noncoding RNA LeXis is a Potential Diagnostic Marker for Non-Alcoholic Steatohepatitis |
title_full | Plasma Long Noncoding RNA LeXis is a Potential Diagnostic Marker for Non-Alcoholic Steatohepatitis |
title_fullStr | Plasma Long Noncoding RNA LeXis is a Potential Diagnostic Marker for Non-Alcoholic Steatohepatitis |
title_full_unstemmed | Plasma Long Noncoding RNA LeXis is a Potential Diagnostic Marker for Non-Alcoholic Steatohepatitis |
title_short | Plasma Long Noncoding RNA LeXis is a Potential Diagnostic Marker for Non-Alcoholic Steatohepatitis |
title_sort | plasma long noncoding rna lexis is a potential diagnostic marker for non-alcoholic steatohepatitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601228/ https://www.ncbi.nlm.nih.gov/pubmed/33022942 http://dx.doi.org/10.3390/life10100230 |
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