Performance of Targeted Library Preparation Solutions for SARS-CoV-2 Whole Genome Analysis

Single next-generation sequencing (NGS) proved to be an important tool for monitoring the SARS-CoV-2 outbreak at the global level Until today, thousands of SARS-CoV-2 genome sequences have been published at GISAID (Global Initiative on Sharing All Influenza Data) but only a portion are suitable for...

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Autores principales: Klempt, Petr, Brož, Petr, Kašný, Martin, Novotný, Adam, Kvapilová, Kateřina, Kvapil, Petr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601271/
https://www.ncbi.nlm.nih.gov/pubmed/33003465
http://dx.doi.org/10.3390/diagnostics10100769
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author Klempt, Petr
Brož, Petr
Kašný, Martin
Novotný, Adam
Kvapilová, Kateřina
Kvapil, Petr
author_facet Klempt, Petr
Brož, Petr
Kašný, Martin
Novotný, Adam
Kvapilová, Kateřina
Kvapil, Petr
author_sort Klempt, Petr
collection PubMed
description Single next-generation sequencing (NGS) proved to be an important tool for monitoring the SARS-CoV-2 outbreak at the global level Until today, thousands of SARS-CoV-2 genome sequences have been published at GISAID (Global Initiative on Sharing All Influenza Data) but only a portion are suitable for reliable variant analysis. Here we report on the comparison of three commercially available NGS library preparation kits. We discuss advantages and limitations from the perspective of required input sample quality and data quality for advanced SARS-CoV-2 genome analysis.
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spelling pubmed-76012712020-11-01 Performance of Targeted Library Preparation Solutions for SARS-CoV-2 Whole Genome Analysis Klempt, Petr Brož, Petr Kašný, Martin Novotný, Adam Kvapilová, Kateřina Kvapil, Petr Diagnostics (Basel) Communication Single next-generation sequencing (NGS) proved to be an important tool for monitoring the SARS-CoV-2 outbreak at the global level Until today, thousands of SARS-CoV-2 genome sequences have been published at GISAID (Global Initiative on Sharing All Influenza Data) but only a portion are suitable for reliable variant analysis. Here we report on the comparison of three commercially available NGS library preparation kits. We discuss advantages and limitations from the perspective of required input sample quality and data quality for advanced SARS-CoV-2 genome analysis. MDPI 2020-09-29 /pmc/articles/PMC7601271/ /pubmed/33003465 http://dx.doi.org/10.3390/diagnostics10100769 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Klempt, Petr
Brož, Petr
Kašný, Martin
Novotný, Adam
Kvapilová, Kateřina
Kvapil, Petr
Performance of Targeted Library Preparation Solutions for SARS-CoV-2 Whole Genome Analysis
title Performance of Targeted Library Preparation Solutions for SARS-CoV-2 Whole Genome Analysis
title_full Performance of Targeted Library Preparation Solutions for SARS-CoV-2 Whole Genome Analysis
title_fullStr Performance of Targeted Library Preparation Solutions for SARS-CoV-2 Whole Genome Analysis
title_full_unstemmed Performance of Targeted Library Preparation Solutions for SARS-CoV-2 Whole Genome Analysis
title_short Performance of Targeted Library Preparation Solutions for SARS-CoV-2 Whole Genome Analysis
title_sort performance of targeted library preparation solutions for sars-cov-2 whole genome analysis
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601271/
https://www.ncbi.nlm.nih.gov/pubmed/33003465
http://dx.doi.org/10.3390/diagnostics10100769
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