LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium

Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then qu...

Descripción completa

Detalles Bibliográficos
Autores principales: Wellkamp, Marvin, García-Camacho, Francisco, Durán-Riveroll, Lorena M., Tebben, Jan, Tillmann, Urban, Krock, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601433/
https://www.ncbi.nlm.nih.gov/pubmed/33003497
http://dx.doi.org/10.3390/md18100497
Descripción
Sumario:Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM.

Ejemplares similares