LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium

Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then qu...

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Autores principales: Wellkamp, Marvin, García-Camacho, Francisco, Durán-Riveroll, Lorena M., Tebben, Jan, Tillmann, Urban, Krock, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601433/
https://www.ncbi.nlm.nih.gov/pubmed/33003497
http://dx.doi.org/10.3390/md18100497
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author Wellkamp, Marvin
García-Camacho, Francisco
Durán-Riveroll, Lorena M.
Tebben, Jan
Tillmann, Urban
Krock, Bernd
author_facet Wellkamp, Marvin
García-Camacho, Francisco
Durán-Riveroll, Lorena M.
Tebben, Jan
Tillmann, Urban
Krock, Bernd
author_sort Wellkamp, Marvin
collection PubMed
description Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM.
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spelling pubmed-76014332020-11-01 LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium Wellkamp, Marvin García-Camacho, Francisco Durán-Riveroll, Lorena M. Tebben, Jan Tillmann, Urban Krock, Bernd Mar Drugs Article Amphidinols are polyketides produced by dinoflagellates suspected of causing fish kills. Here, we demonstrate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the identification and quantification of amphidinols (AM). Novel AM were detected by neutral loss (NL) scan and then quantified together with known AM by selection reaction monitoring (SRM). With the new method, AM were detected in four of eight analyzed strains with a maximum of 3680 fg toxin content per cell. In total, sixteen novel AM were detected by NL scan and characterized via their fragmentation patterns. Of these, two substances are glycosylated forms. This is the first detection of glycosylated AM. MDPI 2020-09-29 /pmc/articles/PMC7601433/ /pubmed/33003497 http://dx.doi.org/10.3390/md18100497 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wellkamp, Marvin
García-Camacho, Francisco
Durán-Riveroll, Lorena M.
Tebben, Jan
Tillmann, Urban
Krock, Bernd
LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium
title LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium
title_full LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium
title_fullStr LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium
title_full_unstemmed LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium
title_short LC-MS/MS Method Development for the Discovery and Identification of Amphidinols Produced by Amphidinium
title_sort lc-ms/ms method development for the discovery and identification of amphidinols produced by amphidinium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7601433/
https://www.ncbi.nlm.nih.gov/pubmed/33003497
http://dx.doi.org/10.3390/md18100497
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